Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon

Bower, Neil I.; Johnston, Ian A.

doi:10.1152/physiolgenomics.00065.2010

Cited by 58 publications

(66 citation statements)

References 92 publications

(101 reference statements)

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“…myf5, a member of the MRF family involved in muscle differentiation and proliferation (Chen and Tsai, 2008), showed a clear response to fasting-refeeding with downregulation during fasting and gradual upregulation with feeding (supplementary material Fig.S3). This pattern of myf5 expression was independent of the zebrafish lineage and has been previously described in Atlantic salmon (Bower and Johnston, 2010). Likewise, a transient increase MyoD1b expression with re-feeding has previously been reported in Atlantic salmon (Bower and Johnston, 2010).…”

Section: Discussionsupporting

confidence: 83%

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Experimental selection for body size at age modifies early-life history traits and muscle gene expression in adult zebrafish

Amaral

Johnston

2012

Journal of Experimental Biology

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SUMMARYThe short generation time of the zebrafish (Danio rerio) was exploited to investigate the effects of selection for body size at age on early life-history traits and on the transcriptional response to a growth stimulus in skeletal muscle of adult fish. Replicate populations were either unselected (U-lineage) or subjected to four generations of experimental selection for small (S-lineage) or large (L-lineage) body size at 90days post-fertilization. Body mass was on average 16.3% and 41.0% higher in the L-than in the U-and S-lineages, respectively. Egg diameter was 6.4% lower with 13% less yolk in the S-lineage compared with the other lineages. Maternal transcripts for igf2r, bmpr1aa, igf1ar, igf2a, igfbp5a, ghra and igfbp3 in 2-4 cell stage embryos were higher in the L-than in the S-lineage. Larvae from the L-lineage were significantly larger, but survivorship at the end of the first month was similar between lineages. Gene expression was measured in the fast muscle of adult fish fasted for 7days and then re-fed to satiation for 48h. The expression of 11 insulin-like growth factor pathway genes and 12 other nutritionally responsive genes was similar for the S-and L-lineages as was gut fullness with feeding. Transcript abundance for four genes (igf1a, igf2r, igfbp1a and igfbp1b) showed either regulated or constitutive differences between the S-and L-lineages. For example, igf2 receptor transcript abundance was higher and igbp1a/b transcript abundance was lower in the L-than in the S-lineage, consistent with an effect of selection on insulin-like growth factor signalling. Supplementary material available online at

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Section: Discussionsupporting

confidence: 83%

“…This pattern of myf5 expression was independent of the zebrafish lineage and has been previously described in Atlantic salmon (Bower and Johnston, 2010). Likewise, a transient increase MyoD1b expression with re-feeding has previously been reported in Atlantic salmon (Bower and Johnston, 2010).…”

Section: Discussionsupporting

confidence: 83%

Experimental selection for body size at age modifies early-life history traits and muscle gene expression in adult zebrafish

Amaral

Johnston

2012

Journal of Experimental Biology

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“…The first aim of this study was to test the hypothesis that retained paralogues of IGF-system genes were differentially regulated following transition from a catabolic to an anabolic state. Fasting has been shown to result in an upregulation of muscle IGF receptors in a number of teleost species and is probably a response to a decrease in circulating IGF hormone levels (Chauvigne et al, 2003;Gabillard et al, 2006;Bower et al, 2008;Bower and Johnston, 2010b). In rainbow trout, prolonged fasting resulted in an upregulation of IGFR-Ia, but not IGFR-Ib (Chauvigne et al, 2003) whereas in zebrafish both IGFR paralogues were upregulated (Fig.4A,B).…”

Section: The Journal Of Experimental Biologymentioning

confidence: 89%

“…The screening criteria used to build lists of differentially regulated genes were apparently robust because all 14 genes tested were validated by qPCR and were well correlated (R0.79; supplementary material Fig.S1). Fasting in zebrafish (Fig.6A,B) and Atlantic salmon (Bower et al, 2008;Bower and Johnston, 2010b) is associated with a large increase in the abundance of E3 ubiquitin ligases MURF1 and fbxo32 transcripts. In mammals, the ubiquitin substrate recognition system has been implicated in specific degradation of myoD (Tintignac et al, 2005;Finn and Dice, 2006) and other promyogenic transcription factors (Finn and Dice, 2006).…”

Section: Genome-wide Transcriptional Regulation With Catabolic To Anamentioning

confidence: 91%

“…In zebrafish, the chaperones Hsp90a and Unc45 are coregulated and involved in the folding of the globular head of myosin during myofibrillargenesis, associating with the Z-line once myofibrillar assembly is completed (Etard et al, 2008). The sequencing of subtractive cDNA libraries from fast skeletal muscle of Atlantic salmon fed either maintenance or satiating rations also revealed that expression of chaperone proteins indicative of unfolded protein response (UPR) pathways such as DNAJ4, HSPA1B, HSP90A and CHAC1 was an early response to increased food intake and growth (Bower and Johnston, 2010b). Accumulation of unfolded proteins can occur when the amounts of newly synthesized proteins exceeds that of the protein folding capacity of the endoplasmic reticulum (Okada et al, 2002).…”

Section: Genome-wide Transcriptional Regulation With Catabolic To Anamentioning

confidence: 99%

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Insulin-like growth factor (IGF) signalling and genome-wide transcriptional regulation in fast muscle of zebrafish following a single-satiating meal

Amaral

Johnston

2011

Journal of Experimental Biology

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SUMMARY Male zebrafish (Danio rerio) were fasted for 7 days and fed to satiation over 3 h to investigate the transcriptional responses to a single meal. The intestinal content at satiety (6.3% body mass) decreased by 50% at 3 h and 95% at 9 h following food withdrawal. Phosphorylation of the insulin-like growth factor (IGF) signalling protein Akt peaked within 3 h of feeding and was highly correlated with gut fullness. Retained paralogues of IGF hormones genes were regulated with feeding, with igf1a showing a pronounced peak in expression after 3 h and igf2b after 6 h. Igf-I receptor transcripts were markedly elevated with fasting, and decreased to their lowest levels 45 min after feeding. igf1rb transcripts increased more quickly than igf1ra transcripts as the gut emptied. Paralogues of the insulin-like growth factor binding proteins (IGFBPs) were constitutively expressed, except for igfbp1a and igfbp1b transcripts, which were significantly elevated with fasting. Genome-wide transcriptional responses were analysed using the Agilent 44K oligonucleotide microarray and selected genes validated by qPCR. Fasting was associated with the upregulation of genes for the ubiquitin-proteasome degradation pathway, anti-proliferative and pro-apoptotic genes. Protein chaperones (unc45b, hspd1, hspa5, hsp90a.1, hsp90a.2) and chaperone interacting proteins (ahsa1 and stip1) were upregulated 3 h after feeding along with genes for the initiation of protein synthesis and mRNA processing. Transcripts for the enzyme ornithine decarboxylase 1 showed the largest increase with feeding (11.5-fold) and were positively correlated with gut fullness. This study demonstrates the fast nature of the transcriptional responses to a meal and provides evidence for differential regulation of retained paralogues of IGF signalling pathway genes.

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Effect of sexual maturation on muscle gene expression of rainbow trout: RNA-Seq approach

et al. 2013

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Muscle degradation occurs as a response to various physiological states that are regulated by specific molecular mechanisms. Previously, we characterized the metabolic changes of muscle deterioration of the female rainbow trout at full sexual maturity and spawning (Salem et al., Physiol. Genomics 2006;28:33–45; J. Proteomics 2010;73:778–789). Muscle deterioration in this model represents nutrient mobilization as a response to the energetic overdemands of the egg/ovarian growth phase. Our recent studies showed that most of the changes in muscle growth and quality start 2–3 months before spawning. Gravid fish exhibited reduced intramuscular fat that is lower in saturated and monounsaturated fatty acids and higher in polyunsaturated fatty acids compared to sterile fish. In this study, RNA-Seq was used to explain the mechanisms underlying changes during this phase of sexual maturity. Furthermore, to minimize changes due to nutrient deficits, fish were fed on a high-plane of nutrition. The RNA-Seq technique identified a gene expression signature that is consistent with metabolic changes of gravid fish. Gravid fish exhibited increased abundance of transcripts in metabolic pathways of fatty acid degradation and up-regulated expression of genes involved in biosynthesis of unsaturated fatty acids. In addition, increased expression of genes involved in the citric acid cycle and oxidative phosphorylation was observed for gravid fish. This muscle transcriptomic signature of fish fed on a high nutritional plane is quite distinct from that previously described for fish at terminal stages of maturity and suggest that female rainbow trout approaching spawning, on high nutritional planes, likely mobilize intramuscular fat rather than protein to support gonadal maturation.

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Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon

Abstract: Bower NI, Johnston IA. Discovery and characterization of nutritionally regulated genes associated with muscle growth in Atlantic salmon.

Cited by 58 publications

References 92 publications

Experimental selection for body size at age modifies early-life history traits and muscle gene expression in adult zebrafish

Experimental selection for body size at age modifies early-life history traits and muscle gene expression in adult zebrafish

Insulin-like growth factor (IGF) signalling and genome-wide transcriptional regulation in fast muscle of zebrafish following a single-satiating meal

Effect of sexual maturation on muscle gene expression of rainbow trout: RNA-Seq approach

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