Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis

Yu, Guotai; Champouret, Nicolas; Steuernagel, Burkhard; Olivera, Pablo D.; Simmons, Jamie; Williams, Cole; Johnson, Ryan C.; Moscou, Matthew J.; Hernández-Pinzón, Inmaculada; Green, Phon; Sela, Hanan; Millet, E.; Jones, Jonathan D. G.; Ward, Eric; Steffenson, Brian J.; Wulff, Brande B. H.

doi:10.1007/s00122-017-2882-8

Cited by 31 publications

(39 citation statements)

References 70 publications

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“…In the Triticeae, important donors of biotic stress resistance loci such as Ae. sharonensis [46] or Ae. longissima [47] should be amenable to TRITEX assembly.…”

Section: Discussionmentioning

confidence: 99%

TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools

Monat

Padmarasu

Lux

et al. 2019

Preprint

160

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Chromosome-scale genome sequence assemblies underpin pan-genomic studies. Recent genome assembly efforts in the large-genome Triticeae crops wheat and barley have relied on the commercial closed-source assembly algorithm DeNovoMagic. We have developed TRITEX, an open-source computational workflow that combines paired-end, mate-pair, 10X Genomics linked-read with chromosome conformation capture sequencing data to construct sequence scaffolds with megabase-scale contiguity ordered into chromosomal pseudomolecules. We evaluated the performance of TRITEX on publicly available sequence data of tetraploid wild emmer and hexaploid bread wheat, and constructed an improved annotated reference genome sequence assembly of the barley cultivar Morex as a community resource.

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“…In the Triticeae, important donors of biotic stress resistance loci such as Ae. sharonensis [46] or Ae. longissima [47] should be amenable to TRITEX assembly.…”

Section: Discussionmentioning

confidence: 99%

TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools

Monat

Padmarasu

Lux

et al. 2019

Preprint

160

View full text Add to dashboard Cite

show abstract

“…tritici), such as race TTKSK (Scott et al 2014;Huang et al 2018. More recently, Yu et al (2017) have identified stem rust resistance genes from another member of the sitopsis group, Ae. sharonensis, by generating the first genetic map of Ae.…”

Section: Genomics Of Crop Wild Relativesmentioning

confidence: 99%

Domestication and crop evolution of wheat and barley: Genes, genomics, and future directions

Haas

Schreiber

Mascher

2019

JIPB

112

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Wheat and barley are two of the founder crops of the agricultural revolution that took place 10,000 years ago in the Fertile Crescent and both crops remain among the world's most important crops. Domestication of these crops from their wild ancestors required the evolution of traits useful to humans, rather than survival in their natural environment. Of these traits, grain retention and threshability, yield improvement, changes to photoperiod sensitivity and nutritional value are most pronounced between wild and domesticated forms. Knowledge about the geographical origins of these crops and the genes responsible for domestication traits largely pre‐dates the era of next‐generation sequencing, although sequencing will lead to new insights. Molecular markers were initially used to calculate distance (relatedness), genetic diversity and to generate genetic maps which were useful in cloning major domestication genes. Both crops are characterized by large, complex genomes which were long thought to be beyond the scope of whole‐genome sequencing. However, advances in sequencing technologies have improved the state of genomic resources for both wheat and barley. The availability of reference genomes for wheat and some of its progenitors, as well as for barley, sets the stage for answering unresolved questions in domestication genomics of wheat and barley.

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“…Golden Promise were infected with Pgt race MCCFC or/and TKTTF 10 days after planting. The inoculated plants were rated for disease response 12-14 days after inoculation as previously described in Yu et al, (2017). For Sr33, T 2 plants alongside with the susceptible control cv.…”

Section: Wheat Rust Inoculations and Phenotypic Evaluationsmentioning

confidence: 99%

The wheatSr22,Sr33,Sr35andSr45genes confer resistance against stem rust in barley

Hatta

Johnson

Matny

et al. 2018

Preprint

Self Cite

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SummaryIn the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley cultivation in Africa and Europe. In contrast to wheat with 82 designated stem rust (Sr) resistance genes, barley’s genetic variation for stem rust resistance is very narrow with only seven resistance genes genetically identified. Of these, only one locus consisting of two genes is effective against Ug99, a strain of Pgt which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against Ug99. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium-mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley.

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Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis

Cited by 31 publications

References 70 publications

TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools

TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools

Domestication and crop evolution of wheat and barley: Genes, genomics, and future directions

The wheatSr22,Sr33,Sr35andSr45genes confer resistance against stem rust in barley

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