Discovery and introgression of the wild sunflower-derived novel downy mildew resistance gene Pl 19 in confection sunflower (Helianthus annuus L.)

Zhang, Z. W.; J., G.; Jun, Zhao; Markell, Samuel G.; Qi, Lili

doi:10.1007/s00122-016-2786-z

Cited by 33 publications

(29 citation statements)

References 54 publications

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“…The SNP SFW04052 and SSR ORS963 markers delimited Pl 17 in an interval of 3.0 cM. Zhang et al (2016b) reported the transfer of a new DM gene, Pl 19 from CWR H. annuus from Texas, into cultivated sunflower and mapped this gene downstream from Pl 17 in LG4. Line RHA 464, released by Hulke et al (2010b) for rust resistance based on a source derived from a CWR H. annuus population from California, was also resistant to DM with the Pl arg gene derived from H. argophyllus .…”

Section: Biotic Traitsmentioning

confidence: 99%

Utilization of Sunflower Crop Wild Relatives for Cultivated Sunflower Improvement

2017

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Sunflower (Helianthus annuus L.) is one of the few crops native to the United States. The current USDA–ARS National Plant Germplasm System (NPGS) crop wild relatives sunflower collection is the largest extant collection in the world, containing 2519 accessions comprising 53 species—39 perennial and 14 annual. To fully utilize gene bank collections, however, researchers need more detailed information about the amount and distribution of genetic diversity present within the collection. The wild species are adapted to a wide range of habitats and possess considerable variability for most biotic and abiotic traits. This represents a substantial amount of genetic diversity available for many agronomic traits for cultivated sunflower, which has a very narrow genetic base. Sunflower ranked fifth highest among 13 crops of major importance to global food security surveyed from the mid‐1980s to 2005 in the use of traits from crop wild relatives. The estimated annual economic contribution of the wild species for cultivated sunflower is between US$267 to 384 million. Most of the value is derived from the PET1 cytoplasm from wild H. petiolaris, disease resistance genes, abiotic salt tolerance, and resistance to imidazolinone and sulfonylurea herbicides. Crop wild relatives provide a wide range of valuable attributes for traditional and molecular breeding, as well as for ecological experimentation, and have enabled rapid advances in ecological and evolutionary genetics. The wild species of Helianthus continue to contribute specific traits to combat emerging pests and environmental challenges and, at the same time, are preserved for future generations.

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Section: Biotic Traitsmentioning

confidence: 99%

Utilization of Sunflower Crop Wild Relatives for Cultivated Sunflower Improvement

2017

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“…Unlike BSR, DM resistance is largely governed by pathotype‐specific major genes with complete resistance. More than 20 DM resistance ( R ) genes, denoted by Pl , have been identified in sunflower conferring resistance to one or more pathotypes of P. halstedii, (Mouzeyar et al, 1995; Dußle et al, 2004; Mulpuri et al, 2009; Bachlava et al, 2011; Liu et al, 2012; Vincourt et al, 2012; Qi et al, 2015, 2016; Zhang et al, 2017; Ma et al, 2017). These R genes have been used routinely in commercial sunflower breeding programs for developing DM‐resistant hybrids.…”

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confidence: 99%

Registration of Oilseed Sunflower Germplasms HA‐BSR6, HA‐BSR7, and HA‐BSR8 Highly Resistant to Sclerotinia Basal Stalk Rot and Downy Mildew

Talukder

Long

Seiler

et al. 2019

J of Plant Registrations

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Sclerotinia basal stalk rot (BSR) and downy mildew (DM) are agronomically important diseases of sunflower (Helianthus annuus L.) worldwide. Three sunflower germplasm lines, HA-BSR6 (Reg. no. GP-353, PI 685019), HA-BSR7 (Reg. no. GP-354, PI 685020), and HA-BSR8 (Reg. no. GP-355, PI 685021), were developed with dual resistance to both BSR and DM. Their resistance to BSR was transferred from the wild annual sunflower H. praecox subsp. runyonii Engelmann & A. Gray (Heiser) (PI 468853), and DM resistance was introgressed from inbred line HA 458 possessing the Pl 17 gene. The germplasms were tested for BSR resistance in inoculated field trials in multiple locations over 4 yr. All three germplasm lines showed significantly higher BSR resistance (p < 0.05) than the recurrent parent and the checks across environments, with a 4-yr mean disease incidence (DI) ranging from 1.2 to 4.8%, while 'Cargill 270' (susceptible hybrid check) and HA 89 (recurrent parent) had DI of 36.1 and 31.0%, respectively, and HA 441 (resistant inbred check) and 'Croplan 305' (resistant hybrid check) had DI of 19.5 and 11.6%, respectively. Whole genome scan using genotyping-by-sequencing revealed the presence of H. praecox subsp. runyonii chromosome segments in HA-BSR6, HA-BSR7, and HA-BSR8, which are potentially associated with BSR resistance. Phenotypic evaluation identified DM resistance in all three germplasm lines, with flanking DNA markers confirming the presence of the Pl 17 gene in the lines. These germplasm lines can be used in sunflower breeding programs for pyramiding both BSR and DM resistance genes.

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“…The wild annual H. annuus accession (PI 435414) originally collected from Paris, TX, in 1978 was used as the source of resistance to new virulent races of P. halstedii (Gulya, 2005). The DM resistance gene derived from PI 435414 was named Pl 19 and mapped to sunflower chromosome 4 corresponding to linkage group 4 (Zhang, Ma, Zhao, Markell, & Qi, 2017). The cross between CMS CONFSCLB1 and PI 435414 was made in 2013 and the selected resistant F 1 plants were backcrossed twice to CONFSCLB1.…”

Section: Methodsmentioning

confidence: 99%

“…Four single nucleotide polymorphism (SNP) markers, SFW04052, NSA_006089, C4_6675662, and S4_7964876, for Pl 19 and two SNP markers, NSA_005624 and NSA_009758, diagnostic for Pl 35 were used to confirm the presence of the R genes in HA-DM5 and HA-DM6 (Ma et al, 2019b;Qi et al, 2019;Zhang et al, 2017). DNA isolation and polymerase chain reaction analysis followed the methods described by Qi et al (2016).…”

Section: Dna Marker Testmentioning

confidence: 99%

Registration of two confection sunflower germplasms, HA‐DM5 and HA‐DM6, resistant to sunflower downy mildew

Seiler

2020

J of Plant Registrations

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Downy mildew (DM) is a primary biotic threat to sunflower (Helianthus annuus L.) production worldwide, negatively impacting yield. Germplasms HA‐DM5 (Reg. no. GP‐367, PI 687025) and HA‐DM6 (Reg. no. GP‐368, PI 691507) were developed and released through the collaboration of the USDA‐ARS and the North Dakota State University as new sources of resistance to downy mildew for confection sunflower. The backcross breeding method and DNA marker‐assisted selection were used to develop HA‐DM5 and HA‐DM6. HA‐DM5 is a BC2F3‐derived BC2F4 maintainer selection from the cross of CONFSCLB1*2//CMS CONFSCLB1/ PI 435414 with the DM resistance transferred from a wild H. annuus PI 435414. Phenotypic/molecular characterizations confirmed the presence of the DM resistance gene Pl19 located on sunflower chromosome 4 derived from PI 435414 in HA‐DM5. HA‐DM6 is a composite of seed from individual BC2F4 plants from the cross of CONFSCLB1*2//CMS CONFSCLB1/ PI 494576. The DM resistance in HA‐DM6 is controlled by a single dominant gene Pl35 located on chromosome 1 derived from wild H. argophyllus PI 494576. Both HA‐DM5 and HA‐DM6 confer resistance to several modern P. halstedii races currently identified in the United States and Europe. They provide new genetic diversity in addition to the few currently used DM‐resistant inbred lines in confection sunflower ensuring protection from yield loss.

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Discovery and introgression of the wild sunflower-derived novel downy mildew resistance gene Pl 19 in confection sunflower (Helianthus annuus L.)

Cited by 33 publications

References 54 publications

Utilization of Sunflower Crop Wild Relatives for Cultivated Sunflower Improvement

Utilization of Sunflower Crop Wild Relatives for Cultivated Sunflower Improvement

Registration of Oilseed Sunflower Germplasms HA‐BSR6, HA‐BSR7, and HA‐BSR8 Highly Resistant to Sclerotinia Basal Stalk Rot and Downy Mildew

Registration of two confection sunflower germplasms, HA‐DM5 and HA‐DM6, resistant to sunflower downy mildew

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