Discovery and modification of cytochrome P450 for plant natural products biosynthesis

Liu, Xiaonan; Zhu, Xianfang; Wang, Hui; Liu, Tian; Cheng, Jian; Jiang, Huifeng

doi:10.1016/j.synbio.2020.06.008

Cited by 57 publications

(39 citation statements)

References 111 publications

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“…Over the last few years, there has been remarkable progress in the gene number and function characterization for plant P450s with the rapid development of sequencing technology, omics analysis, and synthetic biology [ 57 ]. Many databases, such as Cytochrome P450 Homepage ( https://drnelson.uthsc.edu/cytochromeP450.html ), Cytochrome P450 Engineering Database ( https://cyped.biocatnet.de ) and Plant GDB ( http://www.plantgdb.org/site/acknowledgments.php ), had been constructed to collect the sequence and function information of plant P450s.…”

Section: Discussionmentioning

confidence: 99%

PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking

Wang

Liu

et al. 2021

Synthetic and Systems Biotechnology

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Section: Discussionmentioning

confidence: 99%

PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking

Wang

Liu

et al. 2021

Synthetic and Systems Biotechnology

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“…Expression of enzymes in microbial systems often has issues of post-translational modification and requires multigene engineering. Genetic elements in plants, such as cytochrome P450 ( Liu et al., 2020 ), are inefficient when transferred to heterogeneous microorganisms. Therefore, it has become a powerful measure to identify the key enzymes and determine the reaction sequence using plant hosts.…”

Section: Elucidation Of Pnp Biosynthetic Pathwaysmentioning

confidence: 99%

“…Plant cytochrome P450s are the largest family of enzymes involved in PNP biosynthesis, and they mainly play the role of monooxygenases in plants ( Liu et al., 2020 ). A new green production plant has been built by coupling P450 enzyme with an in vitro light system or by transforming the P450-dependent pathway into chloroplasts using photosynthetic reducing power to drive the catalytic activity of P450s ( Jensen et al, 2011 ; Nielsen et al, 2013 ; Lassen et al, 2014 ; Mellor et al, 2016 ).…”

Section: Engineered Biosynthesis Of Pnps In Plant Cellsmentioning

confidence: 99%

Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells

Zhu

Liu

et al. 2021

Plant Communications

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Plant natural products (PNPs) are the main sources of drugs, food additives, and new biofuels and have become a hotspot in synthetic biology. In the past two decades, the engineered biosynthesis of many PNPs has been achieved through the construction of microbial cell factories. Alongside the rapid development of plant physiology, genetics, and plant genetic modification techniques, hosts have now expanded from single-celled microbes to complex plant systems. Plant synthetic biology is an emerging field that combines engineering principles with plant biology. In this review, we introduce recent advances in the biosynthetic pathway elucidation of PNPs and summarize the progress of engineered PNP biosynthesis in plant cells. Furthermore, a future vision of plant synthetic biology is proposed. Although we are still a long way from overcoming all the bottlenecks in plant synthetic biology, the ascent of this field is expected to provide a huge opportunity for future agriculture and industry.

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“…Heme-thiolate monooxygenases or CYPs are an interesting class of enzymes that functionalize terpenes through oxygenation reactions (hydroxylation, dealkylation, demethylation, decarboxylation, cyclization, C-C bond cleavage, among others) and present an important opportunity for generating highly decorated natural products. To date, over 300,000 CYPs have been discovered, with less than 1% actually characterized ( Li et al, 2020 ; Liu et al, 2020 ). There is particular interest to produce CYP-derived terpenoids in microbial chassis due to the high barriers of slow growth and costly deconstruction inherent to native plant extraction.…”

Section: Advances In Isoprenoid Functionalizationmentioning

confidence: 99%

Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms

Carruthers

Lee

2021

Front. Microbiol.

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Isoprenoid compounds are biologically ubiquitous, and their characteristic modularity has afforded products ranging from pharmaceuticals to biofuels. Isoprenoid production has been largely successful in Escherichia coli and Saccharomyces cerevisiae with metabolic engineering of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways coupled with the expression of heterologous terpene synthases. Yet conventional microbial chassis pose several major obstacles to successful commercialization including the affordability of sugar substrates at scale, precursor flux limitations, and intermediate feedback-inhibition. Now, recent studies have challenged typical isoprenoid paradigms by expanding the boundaries of terpene biosynthesis and using non-model organisms including those capable of metabolizing atypical C1 substrates. Conversely, investigations of non-model organisms have historically informed optimization in conventional microbes by tuning heterologous gene expression. Here, we review advances in isoprenoid biosynthesis with specific focus on the synergy between model and non-model organisms that may elevate the commercial viability of isoprenoid platforms by addressing the dichotomy between high titer production and inexpensive substrates.

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Discovery and modification of cytochrome P450 for plant natural products biosynthesis

Cited by 57 publications

References 111 publications

PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking

PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking

Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells

Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms

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