Discovery and Occurrence of the Fumonisins: A Historical Perspective

Marasas, Walter F. O.

doi:10.2307/3435014

Cited by 208 publications

(208 citation statements)

References 32 publications

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“…Fumonisin B 1 (FB 1 ) remains the most abundant mycotoxin in naturally contaminated corn-based foods [1,2] and has neurotoxic potential, pulmonary toxicity, liver toxicity, kidney toxicity, immunotoxicity, cytotoxicity, and carcinogenicity [3][4][5][6]. Furthermore, it has been classified as a group 2B human carcinogen by the International Agency for Research on Cancer (IARC) [7].…”

Section: Introductionmentioning

confidence: 99%

Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains

Ren

Huang

et al. 2015

Anal Bioanal Chem

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An immunochromatographic strip (ICS) using urchin-like gold nanoparticles (UGNs) for sensitive detection of fumonisin B1 (FB1) was developed to meet the requirement for rapidly monitoring FB1 in grain samples. The sensitivity of the ICS was 5.0 ng/mL, which represents a fourfold increase in sensitivity over conventional strip preparation using colloidal gold as the antibody-labeled probe. Analysis of FB1 in grain samples showed that data obtained from the strip tests were in a good agreement with those obtained from HPLC and enzyme-linked immunosorbent assays (ELISAs). This qualitative test did not require any specialized equipment, and the detection time was less than 5 min, which is suitable for on-site testing of FB1 in grain samples. Overall, to our knowledge, this is the first report of using a UGN as the antibody-labeled probe for sensitive detection of FB1 in grains using an ICS. Graphical Abstract Preparation of ICS using conventional colloidal gold and urchin-like gold nanoparticle, respectively.

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Section: Introductionmentioning

confidence: 99%

Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains

Ren

Huang

et al. 2015

Anal Bioanal Chem

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“…Currently South Africa has no legislation or monitoring system regarding allowable fumonisin concentrations and consumers may be at greater risk due to the higher consumption of maize in comparison to European countries (Marasas 2001). Marasas (2001) recorded large variations in probable daily intake (PDI) of maize ranging from 1.2 μg/kg bodyweight (bw)/day in urban South Africans consuming commercial maize, to 354.9 μg/kg bw/day in rural South Africans consuming moldy, home-grown maize.…”

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confidence: 98%

Fumonisin producing Fusarium spp. and fumonisin contamination in commercial South African maize

et al. 2014

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Fumonisins are secondary, carcinogenic metabolites produced primarily by Fusarium verticillioides and Fusarium proliferatum on maize worldwide. The natural occurrence of fumonisin-producing Fusarium spp. and fumonisin contamination of maize grain were quantified in selected maize cultivars from principal production areas of South Africa. Grain colonization by Fusarium spp. was determined using quantitative real-time PCR (qPCR) and contamination with fumonisins using HPLC analysis. Kernels from the 2007 samples were also plated onto Fusarium selective medium and subsequently, split plates containing PDA & CLA. The number of fumonisin producing Fusarium spp. were quantified and microscopically identified after 14 days. Simple linear regression analysis was used to determine the relationship between target DNA, fumonisins and the number of fumonisin producing Fusarium spp. using the plating out method. Results indicated high natural infection by fumonisinproducing Fusarium spp. and fumonisin concentrations in warmer production areas such as Northern Cape, North West and Free State Provinces. Spearman Ranking Correlations indicated that the responses of cultivars to colonization of grain by fumonisin producing Fusarium spp. varied over localities/seasons (r s =0.42 to 0.64) suggesting that cultivars reacted differently to different environmental/inoculum conditions (disease potentials). Cultivars CRN3505 and DKC80-12B showed a degree of resistance to fungal infection. As with fungal colonization, Spearman Rank Correlations indicated the response of cultivars to fumonisin contamination to vary over localities/ seasons (r s =0.29 to 0.70). Cultivars DKC80-12B and LS8521B showed a degree of resistance to fumonisin contamination. Regression analysis yielded a significant relationship between HPLC data and qPCR, but not with the plating out of grain data suggesting the former to be a better indicator of potential fumonisin contamination. Site-specific, daily maximum temperature and rainfall data were provided by the ARC-Institute for Soil Water and Climate's meteorology office. No significant relationship between these weather parameters and colonization of grain by fumonisin producing Fusarium spp. was recorded, although a tendency was observed between fumonisin contamination and mean maximum temperature.

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“…Fusarium verticillioides produces a broad variety of mycotoxins and other secondary metabolites (Nelson et al 1993;Musser and Plattner 1997;Bezuidenhout et al 1988;Marasas et al 2001;Desjardins 2006; Bartók et al 2006Bartók et al , 2008Bartók et al , 2010Voss et al 2007;Szécsi et al 2010), among which fumonisins are the most recently discovered cytotoxic and carcinogenic mycotoxins Marasas 2001;Proctor et al 2004). The contamination of maize by fumonisin-producing Fusarium strains is not always detected early, because fungal colonization may not cause visible disease symptoms in the field (Bacon and Hinton 1996).…”

Section: Introductionmentioning

confidence: 96%

Identification of airborne propagules of the Gibberella fujikuroi species complex during maize production

et al. 2011

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The airborne dispersal of the anamorphs of the Gibberella fujikuroi species complex was studied under pre-and postharvest maize (corn) production conditions using a 3-stage Andersen sampler. The aim of this study was to identify and analyse the size distribution of such species in air samples. Differences were observed between the concentration of large-and small-sized propagules (identified as aggregates and single microconidia, respectively), but the difference was only significant during a high concentration period (October 2007, P = 0.009). No correlation was found between the concentration of fusaria found at different sampling heights (10 and 150 cm above ground level). Fusarium isolates were collected and identified based on morphological characters and using species-specific PCR assays. The PCR analysis confirmed morphological identification of F. verticillioides, F. proliferatum and F. subglutinans. High concentrations were found during the maize harvest, loading and corn shelling. Our results showed that the monitoring of F. verticillioides should be performed at a single sampling height.

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Discovery and Occurrence of the Fumonisins: A Historical Perspective

Cited by 208 publications

References 32 publications

Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains

Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains

Fumonisin producing Fusarium spp. and fumonisin contamination in commercial South African maize

Identification of airborne propagules of the Gibberella fujikuroi species complex during maize production

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