Discovery of a biomarker for β-Thalassemia by HPLC-MS and improvement from Proton Transfer Reaction – Parallel Ion Parking

Ion parking in native mass spectrometry

“…It has been demonstrated that it is possible to selectively inhibit ion/ion reaction rates in electrodynamic ion traps to allow for ions distributed across a range of charge states to be largely concentrated into a narrower range of charge states in techniques referred to as ion parking, 41 parallel ion parking, 42 or valet ion parking, 43 depending upon the application. Ion parking techniques have been demonstrated, for example, to be useful for concentrating ion signals into a single charge state for subsequent MS/ MS, [44][45][46][47][48] for quantitation of biotherapeutic proteins, 49 and for inhibiting sequential reactions in electron transfer dissociation studies. 50,51 To date, ion parking has not been applied to ions of relatively high m/z values (e.g., >m/z 10 000).…”