Phytoseiidae (Acari: Mesostigmata) is a well-known mite family, and more than 2,700 species have been recorded worldwide. Prior this study, 64 phytoseiid species had been recorded in main island of Taiwan and its neighboring islands. Nevertheless, many areas are still unexplored and need further investigation, such as the Penghu Islands. The present study was based on phytoseiid mite materials collected from the Penghu Islands in 1989 and 2020. A list of identified phytoseiid mites is provided herein, 16 species, eight genera, and three subfamilies. Proprioseiopsis penghuensis sp. nov. and Neoseiulus xiaomenensis sp. nov. are new to science, and Amblyseius cinctus Corpuz-Raros & Rimando, A. fletcheri Schicha, Phytoseius rachelae Swirski & Shechter are new records for the country. The further comprehensive phytoseiid investigation in Penghu Islands is needed for exploring the relationships among environmental and agricultural changes, and phytoseiid mites.