D ubois et al. (2021) separated the genus Euphlyctis Fitzinger 1843 into Euphlyctis and Phrynoderma. In 2022, Dufresnes et al ( 2022) made a taxonomic reassessment and placed all the species in one genus, Euplyctis. The Kerala Skittering Frog (Euphlyctis kerala) was recently described from The Thattekad Bird Sanctuary, Kerala, and has a restricted distribution south of the Palghat Gap (Dinesh et al. 2021). This frog is associated with freshwater ecosystems, especially ponds close to river tributaries (e.g., the Periyar River) at lower elevations (Frost 2021). Sister species include E. aloysii and E. karaavali from western coastal regions and E. hexadactyla from eastern coastal regions of India (Dinesh et al. 2022). A medium-sized frog, E. kerala is closely related and morphologically very similar to E. aloysii, but the ranges differ (Dinesh et al. 2021). The cryptic nature of frogs in this group makes field identification relatively difficult. The study conducted by Dinesh et al. (2021) presented grouping keys, making it easier to understand the distribution of this newly identified frog. Euphlyctis kerala was listed on the IUCN Red List as Data Deficient due to poor knowledge of their geographical distribution. In the present study, we used molecular tools to identify the distribution patterns of E. kerala from different parts of Kerala, India, by genetically identifying them with the help of mitochondrial 16S rRNA gene sequences. We also encountered and noted the distributions of E. karaavali, E. hexadactyla, E. aloysii, and Euphlyctis cyanophlyctis during this study.As a part of amphibian surveys conducted in 2015-2019 in different parts of Kerala, we collected adult Kerala Skittering Frogs from four new locations (10.638 N, 76.567 E; 9.562 N, 76.351 E; 9.477 N, 76.582 E, and 10.742 N, 76.006 E), mostly from ponds during the early monsoon (June-July). We also collected specimens of E. aloysii and E. karaavali from Kerala and E. hexadactyla and E. cyanophlyctis from eastern coastal regions of Tamil Nadu for genetic comparisons (Fig. 1; Table 1). We took photographs of captured frogs and preserved toe clips in absolute alcohol for molecular work. After receiving antibiotic treatment, frogs were released at sites of capture. Morphological parameters followed Dinesh et al. ( 2021) and measurements were taken with Mitutoyo digital callipers to the nearest 0.1 mm.