Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2

Tokarz, Janina; Mindnich, Rebekka; Norton, William; Möller, Gabriele; Angelis, Martin Hrabé de; Adamski, Jerzy

doi:10.1016/j.mce.2011.10.022

Cited by 43 publications

(48 citation statements)

References 57 publications

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“…Few data are available to help interpret these observations since different stress axis endpoints are rarely measured simultaneously in the same individual but a lack of consistent proportionality between WBIC and CRTW in the same individuals has been reported for guppies (Poecilia reticulata) held under laboratory conditions (Fischer et al, 2014) and differences in the relationship between plasma cortisol and CRTW has been reported between the sexes for zebrafish (Danio rerio; Félix et al, 2013) and in the daffodil cichlid (Neolamprologus pulcher; Ligocki et al, 2015). It may also be pertinent that in 18 zebrafish gills cortisol is converted via cortisone to 20β-hydroxycortisone (Tokarz et al, 2012) and in stressed zebrafish the concentrations of sulphated and glucuronidated 20β -hydroxycortisone release across the gills were found to be an order of magnitude greater than the corresponding conjugates of cortisol (Tokarz et al, 2013). Similar metabolism and release patterns might reasonably be suggested to occur in sticklebacks in which case cortisol itself constitutes only a proportion of the total steroid being transferred from the gills into surrounding water.…”

Section: Discussionmentioning

confidence: 99%

A comparison of two methods for the assessment of stress axis activity in wild fish in relation to wastewater effluent exposure

Pottinger

Williams

Matthiessen

2016

General and Comparative Endocrinology

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Article (refereed) -postprintPottinger, Tom G.; Williams, Richard J.; Matthiessen, Peter. 2016. A comparison of two methods for the assessment of stress axis activity in wild fish in relation to wastewater effluent exposure.Contact CEH NORA team at noraceh@ceh.ac.ukThe NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner.

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Section: Discussionmentioning

confidence: 99%

A comparison of two methods for the assessment of stress axis activity in wild fish in relation to wastewater effluent exposure

Pottinger

Williams

Matthiessen

2016

General and Comparative Endocrinology

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“…The group demonstrated that the enzyme was able to convert cortisone to 20β-hydroxycortisone and accordingly named the gene 20β-HSD type2 (hsd20β2) (35). Subsequently, omhsd17β12l was classified in the hsd20β2 subcluster in a phylogenetic tree built by Tokarz et al (35).…”

Section: Characterization Of 17β-hsd Type 12-likementioning

confidence: 99%

“…has not yet been examined; nevertheless, the authors suggested that drhsd20β2 was not likely to play a role in reproduction-related steroid biosynthesis, owing to its ubiquitous expression pattern in all the tissues examined, both in adult fish and throughout embryogenesis (35).…”

Section: Characterization Of 17β-hsd Type 12-likementioning

confidence: 99%

17β-HSD type 12-like is responsible for maturation-inducing hormone synthesis during oocyte maturation in masu salmon¹²

et al. 2016

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Abbreviated title: 17β-HSD12-like responsible for DHP production Key terms: 17α,20β-dihydroxy-4-pregnen-3-one, 20β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase, Oocyte maturation, Granulosa, Masu salmon, Oncorhynchus masou AbstractThe maturation-inducing hormone 17α,20β-dihydroxy-4-pregnen-3-one (DHP) was first identified in the amago salmon. However, although carbonyl reductase-like 20β-hydroxysteroid dehydrogenase (CR/20β-HSD) was reported to convert 17α-hydroxyprogesterone (17OHP) to DHP in rainbow trout, we previously found that CR/20β-HSD mRNA was not up-regulated in stimulated granulosa cells from masu salmon, which suggested that DHP is synthesized by a different enzyme. Accordingly, the present study aimed to identify the specific 20β-hydroxysteroid dehydrogenase (20β-HSD) responsible for DHP production by granulosa cells during final oocyte maturation in masu salmon. RNA-sequencing was performed on granulosa layers that were isolated from ovarian follicles at one month before ovulation and incubated with or without forskolin, which was used to mimic luteinizing hormone, and ~12 million reads were obtained, which yielded 71,062 contigs of > 100 bp. tBlastx analysis identified one contig (#f103496) as similar to 17β-hydroxysteroid dehydrogenase type 12 (hsd17β12); however, because the fulllength #f103496 sequence was different from hsd17β12, it was termed to hsd17β12-like (hsd17β12l). We found that mammalian cells transfected with full-length hsd17β12l exhibited considerable 20β-HSD activity, as indicated by efficient conversion of exogenous 17OHP to DHP. In addition, we found that hsd17β12l mRNA levels were consistently low in follicles during vitellogenic growth; however, the levels increased significantly during final oocyte maturation. The levels of hsd17β12l mRNA were also considerably increased in granulosa layers in which 20β-HSD activity was induced by salmon pituitary extract. Therefore, we suggest that hsd17β12l, not CR/20β-HSD, is the 20β-HSD responsible for DHP production by granulosa cells in masu salmon during final oocyte maturation.

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“…Analyses of the glucocorticoids excreted from fish identified tetrahydro-derivatives of cortisol and cortisone, including 20β-cortolone, 5β-dihydroxycortisone, cortisol, and cortisone [33]–[35]. Another putatively excreted glucocorticoid, 4-pregnen-17α,20β,21-triol-3,11-dione (20β-hydroxycortisone), was recently identified by us as the product of a cortisone reduction catalyzed by the novel enzyme 20β-HSD type 2 in various fish species [36]. Following the analyses of its expression in zebrafish and the evaluation of its kinetic parameters, we hypothesized a role for 20β-HSD type 2 in cortisol catabolism and stress response in concert with 11β-HSD type 2 [36].…”

Section: Introductionmentioning

confidence: 99%

“…Another putatively excreted glucocorticoid, 4-pregnen-17α,20β,21-triol-3,11-dione (20β-hydroxycortisone), was recently identified by us as the product of a cortisone reduction catalyzed by the novel enzyme 20β-HSD type 2 in various fish species [36]. Following the analyses of its expression in zebrafish and the evaluation of its kinetic parameters, we hypothesized a role for 20β-HSD type 2 in cortisol catabolism and stress response in concert with 11β-HSD type 2 [36]. To confirm this hypothesis, we investigated the regulation of both 11β-HSD type 2 and 20β-HSD type 2 in zebrafish embryos upon cortisol treatment and after physical stress.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish 20β-Hydroxysteroid Dehydrogenase Type 2 Is Important for Glucocorticoid Catabolism in Stress Response

et al. 2013

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Stress, the physiological reaction to a stressor, is initiated in teleost fish by hormone cascades along the hypothalamus-pituitary-interrenal (HPI) axis. Cortisol is the major stress hormone and contributes to the appropriate stress response by regulating gene expression after binding to the glucocorticoid receptor. Cortisol is inactivated when 11β-hydroxysteroid dehydrogenase (HSD) type 2 catalyzes its oxidation to cortisone. In zebrafish, Danio rerio, cortisone can be further reduced to 20β-hydroxycortisone. This reaction is catalyzed by 20β-HSD type 2, recently discovered by us. Here, we substantiate the hypothesis that 20β-HSD type 2 is involved in cortisol catabolism and stress response. We found that hsd11b2 and hsd20b2 transcripts were up-regulated upon cortisol treatment. Moreover, a cortisol-independent, short-term physical stressor led to the up-regulation of hsd11b2 and hsd20b2 along with several HPI axis genes. The morpholino-induced knock down of hsd20b2 in zebrafish embryos revealed no developmental phenotype under normal culture conditions, but prominent effects were observed after a cortisol challenge. Reporter gene experiments demonstrated that 20β-hydroxycortisone was not a physiological ligand for the zebrafish glucocorticoid or mineralocorticoid receptor but was excreted into the fish holding water. Our experiments show that 20β-HSD type 2, together with 11β-HSD type 2, represents a short pathway in zebrafish to rapidly inactivate and excrete cortisol. Therefore, 20β-HSD type 2 is an important enzyme in stress response.

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Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2

Cited by 43 publications

References 57 publications

A comparison of two methods for the assessment of stress axis activity in wild fish in relation to wastewater effluent exposure

A comparison of two methods for the assessment of stress axis activity in wild fish in relation to wastewater effluent exposure

17β-HSD type 12-like is responsible for maturation-inducing hormone synthesis during oocyte maturation in masu salmon¹²

Zebrafish 20β-Hydroxysteroid Dehydrogenase Type 2 Is Important for Glucocorticoid Catabolism in Stress Response

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Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2

Cited by 43 publications

References 57 publications

A comparison of two methods for the assessment of stress axis activity in wild fish in relation to wastewater effluent exposure

A comparison of two methods for the assessment of stress axis activity in wild fish in relation to wastewater effluent exposure

17β-HSD type 12-like is responsible for maturation-inducing hormone synthesis during oocyte maturation in masu salmon12

Zebrafish 20β-Hydroxysteroid Dehydrogenase Type 2 Is Important for Glucocorticoid Catabolism in Stress Response

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17β-HSD type 12-like is responsible for maturation-inducing hormone synthesis during oocyte maturation in masu salmon¹²