2023
DOI: 10.1073/pnas.2217194120
|View full text |Cite
|
Sign up to set email alerts
|

Discovery of a rapidly evolving yeast defense factor, KTD1 , against the secreted killer toxin K28

Abstract: Secreted protein toxins are widely used weapons in conflicts between organisms. Elucidating how organisms genetically adapt to defend themselves against these toxins is fundamental to understanding the coevolutionary dynamics of competing organisms. Within yeast communities, “killer” toxins are secreted to kill nearby sensitive yeast, providing a fitness advantage in competitive growth environments. Natural yeast isolates vary in their sensitivity to these toxins, but to date, no polymorphic genetic factors co… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
3
1

Relationship

1
3

Authors

Journals

citations
Cited by 4 publications
(2 citation statements)
references
References 70 publications
0
2
0
Order By: Relevance
“…To gain dynamic information about trafficking in the endolysosomal system, we used the styryl dye FM4-64, which is useful for visualising membranes in yeast, as it binds phospholipids of the plasma membrane and robustly increases fluorescence (Vida and Emr, 1995). We have recently employed an FM4-64 pulse chase technique combined with Airyscan2 to survey the endosomal system of S. cerevisiae (Andreev et al, 2023). A similar strategy was used to initially label the plasma membrane of D. hansenii cells, then temporally label endocytic trafficking to the lysosome / vacuole ( Figure 6A ).…”
Section: Resultsmentioning
confidence: 99%
“…To gain dynamic information about trafficking in the endolysosomal system, we used the styryl dye FM4-64, which is useful for visualising membranes in yeast, as it binds phospholipids of the plasma membrane and robustly increases fluorescence (Vida and Emr, 1995). We have recently employed an FM4-64 pulse chase technique combined with Airyscan2 to survey the endosomal system of S. cerevisiae (Andreev et al, 2023). A similar strategy was used to initially label the plasma membrane of D. hansenii cells, then temporally label endocytic trafficking to the lysosome / vacuole ( Figure 6A ).…”
Section: Resultsmentioning
confidence: 99%
“…The genetic basis of resistance has been uncovered on few occasions. The most complete assessment of resistance found that the KTD1 gene, and a number of its alleles, provide different levels of protection against the S. cerevisae K28 toxin [ 95 ]. Resistance tends to be towards toxins of cooccurring killer strain, as opposed to formerly unencountered killer phenotypes, suggesting killer-mediated evolution in competitors [ 43 ].…”
Section: Fungal Killer Phenotypes In Populations and Communitiesmentioning
confidence: 99%