Discovery of a <scp><i>DFR</i></scp> gene that controls anthocyanin accumulation in the spiny <i>Solanum</i> group: roles of a natural promoter variant and alternative splicing

Wang, Xing; Chen, Xueping; Luo, Sushan; Ma, Wei; Li, Na; Zhang, Weiwei; Tikunov, Yury; Xuan, Shuxin; Zhao, Jianjun; Wang, Yanhua; Zheng, Gengdi; Yu, Ping; Bai, Yuling; Bovy, Arnaud; Shen, Shuxing

doi:10.1111/tpj.15877

Cited by 34 publications

(14 citation statements)

References 63 publications

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“…The results proved that overexpression of ZbDFR could significantly promote the accumulation of anthocyanins. Similar phenotype had been demonstrated in other plants in previous studies, such as Asparagus officinalis (Liang et al, 2022a, b) and Solanum (Wang et al, 2022a, b). After the overexpression of DFR, the calluses of A. officinalis showed a purplish red and the calluses of Solanum showed a deep purple, which were all the manifestation of a large accumulation of anthocyanins.…”

Section: Discussionsupporting

confidence: 88%

“…A study of Rubus occidentalis also proved that cyanidin‐3‐O‐rutinoside and cyanidin‐3‐O‐glucoside were important to fruit color (Dayeon & Eunmi, 2018). In Triticum aestivum , the accumulation of cyanidin‐3‐O‐rutinoside, cyanidin‐3‐O‐glucoside and other anthocyanins gave the seeds a deeper purplish red color and a higher nutritional value (Wang et al, 2022a, b). All these results made the conclusion that cyanidin‐3‐O‐rutinoside and cyanidin‐3‐O‐glucoside were the key pigment compound for Z. bungeanum peels more convincing.…”

Section: Discussionmentioning

confidence: 99%

“…The functions of the key genes which were selected from the anthocyanin synthesis pathway were also not verified. Wang et al (2022a, b) compared the different pigment compounds, intracellular metabolites and gene expression levels during the whole Z . bungeanum development, but they did not identify the key pigment compounds at each stage.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomics integrated with metabolomics to characterize key pigment compounds and genes related to anthocyanin biosynthesis in Zanthoxylum bungeanum peel

Han,

Sun,

Zhang

et al. 2023

Physiologia Plantarum

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Zanthoxylum bungeanum is an important condiment with high economic value and its peel color is one of the main quality indexes. However, the key pigment compounds and related genes are still unclear affecting the quality control of the plants. In this study, the contents of four types of pigments were measured in Z. bungeanum and flavonoids were identified as the most important pigments. Based on the targeted flavonoid metabolomics of Z. bungeanum peels, 14 key pigment compounds were screened out from 152 flavonoids, among which cyanidin‐3‐O‐rutinoside and cyanidin‐3‐O‐glucoside were the most critical compounds for peel color. They were further verified to be present in nine varieties of Z. bungeanum by HPLC fingerprints. The 14 compounds were all associated with flavonoid and anthocyanin biosynthesis pathways and the 39 differentially expressed genes related to these pathways were annotated and screened based on transcriptomics. The genes ZbDFR, ZbANS, and ZbUFGT were identified as three key genes for anthocyanin synthesis in Z. bungeanum peels. Further qRT‐PCR results confirmed the reliability of transcriptomics and the accuracy of gene screening. Subsequent protein induced expression demonstrated that ZbANS and ZbUFGT were expressed after 12 h induced by IPTG while ZbDFR was expressed after 15 h. Further transient and stable transformation analysis confirmed that both anthocyanin content and the expression of ZbDFR were significantly increased in overexpression Z. bungeanum leaves and Nicotiana benthamiana. The functional effect of stable transformation of ZbDFR was more significant than that of transient transformation with a 7.67‐fold/1.49‐fold difference in total anthocyanin content and a 42.37‐fold/12.32‐fold difference in the expression of ZbDFR. This study provides new insights into the chemical composition and the molecular mechanisms of Z. bungeanum peel color and lays an effective foundation for the color quality control, multi‐purpose utilization of Z. bungeanum and the creation of new germplasm.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Transcriptomics integrated with metabolomics to characterize key pigment compounds and genes related to anthocyanin biosynthesis in Zanthoxylum bungeanum peel

Han,

Sun,

Zhang

et al. 2023

Physiologia Plantarum

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“…Second, the expression of anthocyanin and proanthocyanidin biosynthesis genes DFR, ANS, UGT, LAR, ANR showed a downward trend during petal development (Fig 9). FLS and DFR are key structural genes by which flavonoids enter different synthetic branches (Kubra et al ., 2021; Liu et al ., 2021; Wang et al ., 2022), and overexpression of FLS increases the biosynthesis of flavonols (Park et al ., 2020; Yu et al ., 2021). Heterologous expression of Rosa rugosa RrDFR1 and Petunia hybrida PhDFR in tobacco has been reported to inhibit the expression of the endogenous NtFLS and promote the accumulation of anthocyanins (Luo et al ., 2016).…”

Section: Discussionmentioning

confidence: 99%

Multi-omics analysis reveals the regulatory mechanism of flavonol biosynthesis during the formation of petal color inCamellia nitidissima

Yue

Chu

et al. 2023

Preprint

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Camellia nitidissima is a prized woody flower with golden-yellow flowers, and has high ornamental, medicinal and economic value. Previous works indicated that the content of flavonol accumulated greatly during golden petal formation. However, the molecular mechanism of golden flower formation in C. nitidissima remains largely unknown. In this study, we performed an integrative anlysis of transcriptome, proteome and metablome of petals at five developmental stages to construct the regulatory network during golden flower formation in C. nitidissima. Metablome anlysis showed that two flavonols, the quercetin and kaempferol glycosides, were highly accumulated in the golden petals. Furthermore, transcriptome and proteome sequencing suggested that the expression of flavonol biosynthesis genes or proteins was increased in golden petal stage, whereas expression of anthocyanin and proanthocyanidin genes or proteins were decreased. Six MYB and 20 bHLH genes were identified as potentially involved in flavonoid biosynthesis, and the brassinosteroid (BR) and jasmonate (JA) pathways were positively and negatively corretated with flavonol biosynthesis, respectively. Network correlation analysis suggested close relationships among BR and JA, MYB and bHLH, and the flavonoid pathway and flavonoid metabolites. Overall, this study shows a potential regulatory mechanism of flavonol biosynthesis duing golden petal formation in C. nitidissima.

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“…Flavonoid biosynthetic genes are regulated by special ternary transcriptional factor complexes of MYB-Basic, helix-loop-helix, and WD40 (MBW) ( Xu et al., 2015 ). It has been revealed that mutation in the promotor region of dihydroflavonol-4-reductase (DFR) and alternative splicing of DFR leads to alteration in the accumulation of anthocyanin pigment in the solanum group ( Wang et al., 2022 ). In the chrysanthemum transcriptional factor, cmbHLH2 generates two transcripts: fully transcribed cmbHLH2 full and truncated short-length cmbHLH2 short .…”

Section: Applications Of As In Transcriptional Repressionmentioning

confidence: 99%

Alternative splicing: transcriptional regulatory network in agroforestry

Hussain

Abbas

et al. 2023

Front. Plant Sci.

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Alternative splicing (AS) in plants plays a key role in regulating the expression of numerous transcripts from a single gene in a regulatory pathway. Variable concentrations of growth regulatory hormones and external stimuli trigger alternative splicing to switch among different growth stages and adapt to environmental stresses. In the AS phenomenon, a spliceosome causes differential transcriptional modifications in messenger RNA (mRNAs), resulting in partial or complete retention of one or more introns as compared to fully spliced mRNA. Differentially expressed proteins translated from intron-retaining messenger RNA (mRNAir) perform vital functions in the feedback mechanism. At the post-transcriptional level, AS causes the remodeling of transcription factors (TFs) by the addition or deletion of binding domains to activate and/or repress transcription. In this study, we have summarized the specific role of AS in the regulation of gene expression through repression and activation of the transcriptional regulatory network under external stimuli and switch among developmental stages.

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Discovery of a DFR gene that controls anthocyanin accumulation in the spiny Solanum group: roles of a natural promoter variant and alternative splicing

Cited by 34 publications

References 63 publications

Transcriptomics integrated with metabolomics to characterize key pigment compounds and genes related to anthocyanin biosynthesis in Zanthoxylum bungeanum peel

Transcriptomics integrated with metabolomics to characterize key pigment compounds and genes related to anthocyanin biosynthesis in Zanthoxylum bungeanum peel

Multi-omics analysis reveals the regulatory mechanism of flavonol biosynthesis during the formation of petal color inCamellia nitidissima

Alternative splicing: transcriptional regulatory network in agroforestry

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