Discovery of Cytospora species associated with canker disease of tree hosts from Mount Dongling of China

Abstract

Members of Cytospora encompass important plant pathogens, saprobes and endophytes on a wide range of woody hosts with a worldwide distribution. In the current study, we obtained seven representative isolates from six tree hosts of Betulaceae, Juglandaceae, Rosaceae, Tiliaceae and Ulmaceae in Mount Dongling of China. Based on morphological comparison and phylogenetic analyses using partial ITS, LSU, act, rpb2, tef1-α and tub2 gene sequences, we identified two known species (Cytospora leucostoma and C. pruinopsis) and two novel species (C. coryli and C. spiraeicola). These results represent the first study on Cytospora species associated with canker disease from Mount Dongling of China.

Assessment of Cytospora Isolates From Conifer Cankers in China, With the Descriptions of Four New Cytospora Species

“…Notes : This species is identified by having conidiomata with a column lenticular tissue in the center, and having multiple locules surrounded by a black conceptacle. It can be distinguished from its closest relative C. coryli by the discoid pycnidial stromata with a conspicuous central column, and smaller conidia (4.5–5.5 × 1–1.5 vs. 5–7 × 1–2 μm) (Zhu et al, 2020 ).…”

“…Nevertheless, the species occurrence may be related to geographical and environmental factors rather than host specificity (Fan et al, 2014a , b ; Fan et al, 2015a , b ). To more accurately identify Cytospora species, several species have been described based on morphological observations and multilocus phylogeny in recent studies (Yang et al, 2015 ; Lawrence et al, 2017 , 2018 ; Zhu et al, 2018 , 2020 ; Fan et al, 2020 ; Jiang et al, 2020 ; Shang et al, 2020 ). Norphanphoun et al ( 2017 ) used four loci to describe 14 new species isolated from Rosa, Salix , and Sorbus .…”

The Hidden Diversity of Diatrypaceous Fungi in China

“…Due to overlapping phenotypic characters in Diatrypaceae, polyphasic approaches to solve the taxonomy of fungi were very common in recent studies (Wijayawardene et al, 2016;Fan et al, 2018Fan et al, , 2020Lawrence et al, 2018;Zhu et al, 2020). The first molecular phylogenetic analysis of Diatrypaceae based on ITS showed that Cryptosphaeria, Diatrype, Diatrypella, Eutypa, and Eutypella were polyphyletic (Acero et al, 2004).…”

High Diversity of Cytospora Associated With Canker and Dieback of Rosaceae in China, With 10 New Species Described

“…A total of 144 strains from Iran represented 20 species of Cytospora based on ITS phylogeny ( Fotouhifar et al, 2010 ). Later, many species were described based on multilocus phylogeny in recent studies ( Fan et al, 2014a , b , 2015a , b ; Yang et al, 2015 ; Lawrence et al, 2017 , 2018 ; Zhu et al, 2018 , 2020 ; Jiang et al, 2020 ; Shang et al, 2020 ). Fan et al (2020) provided an assessment of 52 species of Cytospora in China using a six-locus phylogeny [internal transcribed spacer (ITS), large nuclear ribosomal RNA subunit (LSU), actin ( act ), RNA polymerase II subunit ( rpb2 ), translation elongation factor 1-α ( tef1- α), and beta-tubulin ( tub2 )].…”