2007
DOI: 10.1099/vir.0.83045-0
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Discovery of herpesviruses in bats

Abstract: Seven novel gammaherpesviruses (GHV) and one novel betaherpesvirus were discovered in seven different European bat species (order Chiroptera, family Vespertilionidae) with a pan-herpesvirus PCR assay, targeting the DNA polymerase (DPOL) gene. The sequences of six bat GHV were similarly related to members of the gammaherpesvirus genera Percavirus and Rhadinovirus. The seventh GHV was related to the porcine lymphotropic herpesvirus 1 (genus Macavirus). The betaherpesvirus appeared to be a distant relative of hum… Show more

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Cited by 94 publications
(95 citation statements)
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“…The utility of partial sequence analyses has been established previously in resolving the evolutionary relationships among HVs of mammals (Ehlers et al 1999, Ehlers & Lowden 2004, Wibbelt et al 2007) and reptiles , and is extended in the present study to fish and amphibian HVs. Phylogenetic analyses of partial sequences from the DNA polymerase and terminase genes, and of more extensive sequences for a subset of viruses, strongly support the monophyly of fish and amphibian HVs within a larger clade containing all HVs.…”
Section: Discussionmentioning
confidence: 88%
“…The utility of partial sequence analyses has been established previously in resolving the evolutionary relationships among HVs of mammals (Ehlers et al 1999, Ehlers & Lowden 2004, Wibbelt et al 2007) and reptiles , and is extended in the present study to fish and amphibian HVs. Phylogenetic analyses of partial sequences from the DNA polymerase and terminase genes, and of more extensive sequences for a subset of viruses, strongly support the monophyly of fish and amphibian HVs within a larger clade containing all HVs.…”
Section: Discussionmentioning
confidence: 88%
“…To search for herpesvirus DNA sequences present in felids, we performed degenerate nested PCR on felid blood cell DNA samples using primers which target the glycoprotein B (gB) gene of gammaherpesviruses (GHVs) (2,34,35). In our initial analysis, 4 of 60 domestic cat samples were PCR positive, and 453-bp gB sequences amplified from these samples were identical ( Table 2).…”
Section: Resultsmentioning
confidence: 99%
“…1): (i) amplify short segments of DNApol with degenerate panherpesviral PCR to obtain an approximately 170-bp sequence, and (ii) use short gB and DNApol sequences to design virus-specific primers for long-distance PCR. This strategy resulted in the generation of a bigenic sequence of approximately 3.4 kb (34,35,38) and was used to ensure that gB and DNApol sequences were derived from the same virus genome. A common panherpesviral PCR used to target DNApol for virus discovery (26,39) amplified LruGHV1 and bovine herpesvirus 6 GHV DNA, but not FcaGHV1 or PcoGHV1.…”
Section: Resultsmentioning
confidence: 99%
“…The advantage was that these genes are adjacent in GHV genomes, and long-distance PCR (LD-PCR) could be used to close the gap between the generated gB and DPOL sequence information, resulting in a final sequence of approximately 3.4 kbp. This bigenic approach had been used already for the amplification of novel betaherpesviruses and GHVs of primates, artiodactyls, bats, and rodents (3,11,25,33). Here, we used it for a broad analysis of mammalian Gammaherpesvirinae and discovered novel GHVs in eight different host species from six mammalian orders.…”
mentioning
confidence: 99%