Discovery of novel anelloviruses in small mammals expands the host range and diversity of the Anelloviridae

Souza, William Marciel de; Fumagalli, Marcílio Jorge; Araújo, Jansen de; Sabino‐Santos, Gilberto; Maia, Felipe Gonçalves Motta; Romeiro, Marília Farignoli; Modha, Sejal; Nardi, Marcello Schiavo; Queiroz, Luzia Helena; Durigon, Edison Luiz; Nunes, Márcio Roberto Teixeira; Murcia, Pablo R.; Figueiredo, Luíz Tadeu Moraes

doi:10.1016/j.virol.2017.11.001

Cited by 53 publications

(41 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sequencing was performed at the Life Sciences Core Facility from University of Campinas, Brazil. The resulting sequencing reads were assembled de novo using the metaViC pipeline ( https://github.com/sejmodha/MetaViC ) 28 . This pipeline is divided into two major components.…”

Section: Methodsmentioning

confidence: 99%

Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil

Souza

Fumagalli

Carrasco

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

Ticks are ectoparasites spread worldwide and are well known as vectors of many viruses of great importance to human and animal health. However, the viral diversity in ticks is still poorly understood, particularly in South America. Here we characterized the viral diversity present in Rhipicephalus microplus parasitizing cattle in the southern region of Brazil using metagenomics. Our study revealed the presence of viruses that had not been previously described in the region, including lihan tick virus (Phenuiviridae family) and wuhan tick virus 2 (Chuviridae family), as well as expands the biogeography of jingmen tick virus (Flaviviridae family) in Brazil. Also, we described three novel tymoviruses (Tymovirales order), named guarapuava tymovirus-like 1 to 3. We described the genomic and phylogenetic characterization of these viruses. Our study sheds light on the viral diversity of Rhipicephalus microplus in South America, and also expands the biogeography of tick viruses that were previously described only in Asia.

show abstract

Section: Methodsmentioning

confidence: 99%

Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil

Souza

Fumagalli

Carrasco

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tissue samples were individually homogenized with Hank’s balanced salt solution using the TissueLyser system (Qiagen, Germantown, MD, USA). Then, the homogenized tissue, sera and cloacal swabs were centrifuged for 5 min at 10,000 g and the pools were prepared as previously described [ 12 ]. The viral genomes were extracted with a QIAamp viral RNA mini kit (Qiagen, USA) and stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

“…A total of 7,059,398 to 94,508,748 paired-end reads per pool were generated with 64.85% to 91.45% of bases ≥ Q30 with a base call accuracy of 99.9% ( Table S1 ). The sequencing reads were assembled using the de novo approach in the metaViC pipeline ( ) [ 12 ]. The parvovirus contigs longer than length of 200 nucleotides and supercontigs were merged and classified using DIAMOND against NCBI RefSeq protein database [ 13 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

Novel Parvoviruses from Wild and Domestic Animals in Brazil Provide New Insights into Parvovirus Distribution and Diversity

De Souza,

Dennis,

Fumagalli

et al. 2018

Viruses

Self Cite

View full text Add to dashboard Cite

Parvoviruses (family Parvoviridae) are small, single-stranded DNA viruses. Many parvoviral pathogens of medical, veterinary and ecological importance have been identified. In this study, we used high-throughput sequencing (HTS) to investigate the diversity of parvoviruses infecting wild and domestic animals in Brazil. We identified 21 parvovirus sequences (including twelve nearly complete genomes and nine partial genomes) in samples derived from rodents, bats, opossums, birds and cattle in Pernambuco, São Paulo, Paraná and Rio Grande do Sul states. These sequences were investigated using phylogenetic and distance-based approaches and were thereby classified into eight parvovirus species (six of which have not been described previously), representing six distinct genera in the subfamily Parvovirinae. Our findings extend the known biogeographic range of previously characterized parvovirus species and the known host range of three parvovirus genera (Dependovirus, Aveparvovirus and Tetraparvovirus). Moreover, our investigation provides a window into the ecological dynamics of parvovirus infections in vertebrates, revealing that many parvovirus genera contain well-defined sub-lineages that circulate widely throughout the world within particular taxonomic groups of hosts.

show abstract

“…Tissues samples were individually homogenized with Hank’s balanced salt solution using the TissueLyser system (Qiagen, USA). Then, the homogenized tissue, sera, and cloacal swabs were centrifuged by 5 min at 10,000g, and the pools were prepared as previously described [12]. The viral genomes were extracted with a QIAamp viral RNA mini kit (Qiagen, USA) and stored at −80°C.…”

Section: Methodsmentioning

confidence: 99%

“…A total of 7,059,398 to 94,508,748 paired-end reads per pool were generated with 64.85% to 91.45% of bases ≥ Q30 with a base call accuracy of 99.9% (Supplementary Table 1). The sequencing reads were assembled using the de novo approach in the metaViC pipeline (github.com/sejmodha/MetaViC) [12].…”

Section: Methodsmentioning

confidence: 99%

Whole-genome sequencing of parvoviruses from wild and domestic animals in Brazil provides new insights into parvovirus distribution and diversity

Souza

Dennis

Fumagalli

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

Parvoviruses (family Parvoviridae) are small, single-stranded DNA viruses. Many 30 parvoviral pathogens of medical, veterinary and ecological importance have been identified. In 31 this study, we used high-throughput sequencing (HTS) to investigate the diversity of parvoviruses 32 infecting wild and domestic animals in Brazil. We identified 21 parvovirus sequences (including 33 twelve nearly complete genomes and nine partial genomes) in samples derived from rodents, bats, 34 opossums, birds and cattle in Pernambuco, São Paulo, Paraná and Rio Grande do Sul states. These 35 sequences were investigated using phylogenetic and distance-based approaches, and were thereby 36 classified into eight parvovirus species (six of which have not been described previously), 37representing six distinct genera in the subfamily Parvovirinae. Our findings extend the known 38 biogeographic range of previously characterized parvovirus species, and the known host range of 39 three parvovirus genera (Dependovirus, Aveparvovirus, and Tetraparvovirus). Moreover, our 40 investigation provides a window into the ecological dynamics of parvovirus infections in 41 vertebrates, revealing that many parvovirus genera contain well-defined sub-lineages that 42 circulate widely throughout the world within particular taxonomic groups of hosts. 43

show abstract

Discovery of novel anelloviruses in small mammals expands the host range and diversity of the Anelloviridae

Cited by 53 publications

References 47 publications

Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil

Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil

Novel Parvoviruses from Wild and Domestic Animals in Brazil Provide New Insights into Parvovirus Distribution and Diversity

Whole-genome sequencing of parvoviruses from wild and domestic animals in Brazil provides new insights into parvovirus distribution and diversity

Contact Info

Product

Resources

About