Discovery of Potential Biomarkers for Postmenopausal Osteoporosis Based on Untargeted GC/LC-MS

Abstract

PurposeAs an important public health problem, osteoporosis (OP) in China is also in an upward trend year by year. As a standard method for diagnosing OP, dual-energy X-ray absorptiometry (DXA) cannot analyze the pathological process but only see the results. It is difficult to evaluate the early diagnosis of OP. Our study was carried out through a serum metabolomic study of OP in Chinese postmenopausal women on untargeted gas chromatography (GC)/liquid chromatography (LC)–mass spectrometry (MS) to find possible diagnostic markers.Materials and Methods50 Chinese postmenopausal women with osteoporosis and 50 age-matched women were selected as normal controls. We first used untargeted GC/LC-MS to analyze the serum of these participants and then combined it with a large number of multivariate statistical analyses to analyze the data. Finally, based on a multidimensional analysis of the metabolites, the most critical metabolites were considered to be biomarkers of OP in postmenopausal women. Further, biomarkers identified relevant metabolic pathways, followed by a map of metabolic pathways found in the database.ResultsWe found that there may be metabolic pathway disorders like glucose metabolism, lipid metabolism, and amino acid metabolism in postmenopausal women with OP. 18 differential metabolites are considered to be potential biomarkers of OP in postmenopausal women which are a major factor in metabolism and bone physiological function.ConclusionThese findings can be applied to clinical work through further validation studies. It also shows that metabonomic analysis has great potential in the application of early diagnosis and recurrence monitoring in postmenopausal OP women.

[Retracted] The Hub Genes Related to Osteoporosis Were Identified by Bioinformatics Analysis

“…Studies have now shown the presence of biomarkers in the OP, such as some potential biomarkers of OP that have been found in metabolomics studies. These metabolites are mainly concentrated in fats and amino acids [ 9 ]. Studies have shown that circulating miR-181c-5p and miR-497-5p may serve as potential biomarkers to monitor the effect of anti-OP therapy or diagnostic methods [ 10 ].…”

A Multi-Omics Analysis Reveals Anti-Osteoporosis Mechanism of Four Components from Crude and Salt-Processed Achyranthes bidentata Blume in Ovariectomized Rats

“…Compared with the model group, the levels of lactate, citrate, and succinate in the sAB-, ABP-, and sABP-treated groups were decreased. This phenomenon can be explained by the bone resorption of osteoclasts consuming a large amount of energy, which causes the speed up of glycolysis and oxidative phosphorylation [ 24 ]. With the enhancement of energy metabolism, the tricarboxylic acid cycle (TCA) is also enhanced.…”

Exploring serum metabolic profiles in postmenopausal osteoporosis women by using NMR metabolomics and network pharmacology

“…Third-generation alendronate tablets can act on the competitive target of OC to inhibit OC activity and play a role in maintaining bone structure, improving bone mineralisation and bone density [7]. Nonetheless, it is imperative to consider the potential risks of osteonecrosis of the jaw [8] and gastrointestinal discomfort [9] linked to the administration of alendronate tablets in the management of patients with PMOP. Regular assessments of both e cacy and safety are crucial.…”