Discovery of Tropolone Stipitaldehyde as a Potential Agent for Controlling Phytophthora Blight and Its Action Mechanism Research

Wang, Biao; He, Bo; Chen, Tianyu; Li, Hao; Chen, Liyifan; Chen, Yiliang; Tian, Kailin; Yang, Kun; Shen, Danyu; Yan, Wei; Ye, Yonghao

doi:10.1021/acs.jafc.2c03163

Cited by 15 publications

(12 citation statements)

References 51 publications

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“…The effects of EFL 3 on the mycelial morphology and ultrastructure of P. capsici were observed using OM and TEM observations [ 39 ]. Fresh mycelial plugs of LT263 (standard strain of P. capsici ) and TA54 were cut and incubated on V8 juice agar plates supplemented with 0, 5, or 10 μg/mL of EFL 3 .…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Inhibitory Effect and Mechanism of Euphorbia Factor L3 against Phytophthora capsici

Zhang

Yang

et al. 2023

Molecules

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Phytophthora capsici is a highly destructive phytopathogenic oomycete with a broad host range and is responsible for tremendous losses. Euphorbia factor L3 (EFL3) is a natural plant-derived compound that has been widely studied in medicine and cosmetic applications. In this study, the sensitivity of 105 P. capsici isolates to EFL3 was determined, and the biological activity and physiological effects of EFL3 against P. capsici were investigated. The median effective concentration (EC50) values for EFL3 inhibition mycelial growth and spore germination ranged from 0.66 to 8.94 μg/mL (mean, 2.96 ± 0.91 μg/mL) and 1.63 to 13.16 μg/mL (mean, 5.30 ± 1.64 μg/mL), respectively. EFL3 treatment resulted in cell wall and cell membrane damage of P. capsici, which was revealed by morphological and ultrastructural observations, propidium iodide (PI) and calcofluor white (CFW) staining, and measurements of relative conductivity as well as malondialdehyde (MDA) and glycerol contents. In addition, the contents of phospholipid and cellulose, which are the major components of cell membrane and cell wall, were significantly reduced following EFL3 treatment. Furthermore, EFL3 provided protective as well as curative efficacies against P. capsici on detached tomato leaves and pepper seedlings in vivo. These data show that EFL3 exhibits strong inhibitory activity against P. capsici, thereby suggesting that it could be an effective alternative for controlling P. capsici-induced diseases.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Inhibitory Effect and Mechanism of Euphorbia Factor L3 against Phytophthora capsici

Zhang

Yang

et al. 2023

Molecules

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“…The relative conductivity of mycelia was calculated as below: relative conductivity (%) = [(conductivity at different times − initial conductivity)/the final conductivity] × 100%. 33 The experiment was conducted in triplicate, and per treatment had three replicates. Malondialdehyde Content Assays.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…After being incubated for 12 h, the mycelia were boiled for 10 min and were considered as the final conductivity. The relative conductivity of mycelia was calculated as below: relative conductivity (%) = [(conductivity at different times – initial conductivity)/the final conductivity] × 100% . The experiment was conducted in triplicate, and per treatment had three replicates.…”

Section: Methodsmentioning

confidence: 99%

“…The membrane permeability of F. graminearum PH-1 cells as affected by VentA was evaluated by a conductivity method. , Briefly, 1 mL of fresh conidial suspensions (1 × 10 5 mL –1 ) was added into YEPD medium (100 mL) and rotated at 28 °C, 180 rpm for 24 h. Then, the resulting mycelial samples were harvested, rinsed four times with sterile water, and filtered with a filtering device to eliminate residual water. Next, 1 g of the fresh mycelia was resuspended in 25 mL of sterile water containing the EC 50 , 20, and 40 μg/mL of VentA.…”

Section: Methodsmentioning

confidence: 99%

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Venturicidin A Is a Potential Fungicide for Controlling Fusarium Head Blight by Affecting Deoxynivalenol Biosynthesis, Toxisome Formation, and Mitochondrial Structure

Hu,

Guo,

Chen

et al. 2023

J. Agric. Food Chem.

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Fusarium graminearum, which causes Fusarium head blight (FHB) in cereals, is one of the most devastating fungal diseases by causing great yield losses and mycotoxin contamination. A major bioactive ingredient, venturicidin A (VentA), was isolated from Streptomyces pratensis S10 mycelial extract with an activity-guided approach. No report is available on antifungal activity of VentA against F. graminearum and effects on deoxynivalenol (DON) biosynthesis. Here, VentA showed a high antagonistic activity toward F. graminearum with an EC 50 value of 3.69 μg/mL. As observed by scanning electron microscopy, after exposure to VentA, F. graminearum conidia and mycelia appeared abnormal. Different dyes staining revealed that VentA increased cell membrane permeability. In growth chamber and field trials, VentA effectively reduced disease severity of FHB. Moreover, VentA inhibited DON biosynthesis by reducing pyruvic acid, acetyl-CoA production, and accumulation of reactive oxygen species (ROS) and then inhibiting trichothecene (TRI) genes expression and toxisome formation. These results suggest that VentA is a potential fungicide for controlling FHB.

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“…The total RNA of P. capsici was extracted using the TRIzol Reagent (Invitrogen, CA, USA). 31 The library preparation and sequencing were entrusted to Novogene (Beijing, China). A reference genome of P. capsici was downloaded (https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_030324255.…”

Section: Transcriptome Analysismentioning

confidence: 99%

Antifungal activity of the botanical compound rhein against Phytophthora capsici and the underlying mechanisms

Wang,

Yang,

Zhao

et al. 2023

Pest Management Science

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BackgroundPhytophthora capsici is an extremely destructive phytopathogenic oomycete that causes huge economic losses. However, due to the drug resistance risk and environmental threat of chemical fungicides, it is necessary to develop environmentally friendly biocontrol alternatives. Rhein is a major medicinal ingredient of traditional Chinese herbs, and it is widely used in medical field. However, its inhibitory effect against phytopathogens is unknown. Herein, the antifungal spectrum of rhein and its possible action mechanism against P. capsici were investigated.ResultsRhein possessed broad‐spectrum antifungal activity against phytopathogens, particularly P. capsici, Phytophthora infestans, Helminthosporium maydis, and Rhizoctonia solani. Rhein inhibited the mycelial growth as well as the spore germination of P. capsici with mean 50% effective concentration (EC50) values of 4.68 μg mL‐1 and 6.57 μg mL‐1 against 117 P. capsici isolates, respectively. Rhein effectively suppressed the occurrence and spread of Phytophthora blight and significantly destroyed the cell membrane permeability and integrity of P. capsici, corroded its cell wall integrity, and damaged its morphology and ultrastructure. Moreover, rhein caused a considerable reduction in the phospholipid and cellulose contents. Genome‐wide transcriptional profiling of P. capsici in response to rhein indicated significant reduction in the expression levels of genes participating in glycerolipid metabolism and starch and sucrose metabolism. Additionally, rhein strengthened the disease defense system of pepper by enhancing related enzyme activities.ConclusionThis study demonstrated that rhein could effectively inhibit P. capsici using multiple mechanisms of action. Rhein has the potential to be an efficient alternative to control diseases caused by P. capsici.This article is protected by copyright. All rights reserved.

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Discovery of Tropolone Stipitaldehyde as a Potential Agent for Controlling Phytophthora Blight and Its Action Mechanism Research

Cited by 15 publications

References 51 publications

Evaluation of Inhibitory Effect and Mechanism of Euphorbia Factor L3 against Phytophthora capsici

Evaluation of Inhibitory Effect and Mechanism of Euphorbia Factor L3 against Phytophthora capsici

Venturicidin A Is a Potential Fungicide for Controlling Fusarium Head Blight by Affecting Deoxynivalenol Biosynthesis, Toxisome Formation, and Mitochondrial Structure

Antifungal activity of the botanical compound rhein against Phytophthora capsici and the underlying mechanisms

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