Discovery of Two New <i>Talaromyces</i> Species from Crop Field Soil in Korea

Yadav, Dil Raj; Kim, Sangwoo; Um, Yong Hyun; Kim, Hyung Seung; Lee, Hyang Burm; Lee, Youn Su

doi:10.5941/myco.2015.43.4.402

Cited by 8 publications

(6 citation statements)

References 12 publications

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“…All six acidophilic fungi were clustered in the branch of the genus Talaromyces. We summarized all the references on isolation and identification of Talaromyces since 2015 and found that no acidic culture conditions were involved in the isolation and culture process (including 34 new species of Talaromyces) (Adhikari et al, 2015;Visagie et al, 2015;Chen et al, 2016;Luo et al, 2016;Yilmaz et al, 2016a,b;Barbosa et al, 2018;Jiang et al, 2018;Rajeshkumar et al, 2019). However, in our experiments, all six acidophilic fungi isolates belonged to the genus Talaromyces.…”

Section: Isolation and Phylogenetic Diversity Of Aciduric Fungi Associated With Thai Mangrove Sedimentmentioning

confidence: 54%

Bioactive Metabolites From Acid-Tolerant Fungi in a Thai Mangrove Sediment

et al. 2021

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Despite being potentially useful extremophile resources, there have been few reports on acid-tolerant fungi and their bioactive metabolites. Acidophilic/aciduric fungi (n = 237) were isolated from Thai mangrove sediments in an acidic medium. Using fungal identification technology (including morphologic observation, chemical screening, and sequence comparisons) all the isolates were identified and 41 representative isolates were selected for analysis of the phylogenetic relationships (ITS rDNA, β-tubulin, calmodulin, and actin gene sequences). There were seven genera identified – Penicillium; Aspergillus; Talaromyces; Cladosporium; Allophoma; Alternaria; and Trichoderma – in four taxonomic orders of the phylum Ascomycota, and Penicillium, Aspergillus, and Talaromyces were the dominant genera. Acidity tolerance was evaluated and 95% of the isolates could grow under extremely acidic conditions (pH 2). Six strains were classed as acidophilic fungi that cannot survive under pH 7, all of which had an extraordinarily close genetic relationship and belonged to the genus Talaromyces. This is the first report on the acidophilic characteristics of this genus. The antimicrobial, anti-tumor, and antiviral activities of the fermentation extracts were evaluated. Nearly three-quarters of the extracts showed cytotoxic activity, while less than a quarter showed antimicrobial or anti-H1N1 activity. The typical aciduric fungus Penicillium oxalicum OUCMDZ-5207 showed similar growth but completely different chemical diversity at pH 3 and 7. The metabolites of OUCMDZ-5207 that were obtained only at pH 3 were identified as tetrahydroauroglaucin (1), flavoglaucin (2), and auroglaucin (3), among which auroglaucin showed strong selective inhibition of A549 cells with an IC50 value of 5.67 μM. These results suggest that acid stress can activate silent gene clusters to expand the diversity of secondary metabolites, and the bioprospecting of aciduric/acidophilic microorganism resources in Thai mangrove sediments may lead to the discovery of compounds with potential medicinal applications.

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Section: Isolation and Phylogenetic Diversity Of Aciduric Fungi Associated With Thai Mangrove Sedimentmentioning

confidence: 54%

Bioactive Metabolites From Acid-Tolerant Fungi in a Thai Mangrove Sediment

et al. 2021

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“…Of the lineages selected, it is known that A. parasiticus is a toxigenic fungus with the potential for aflatoxin production, but it must be remembered that mycotoxin production is influenced by complex interactions between abiotic and biotic factors (water activity, temperature, pH, moisture, and storage time) (Copetti et al 2011a;Garcia et al 2011). Researchers have evaluated the occurrence and distribution of aflatoxigenic species as well as the production of aflatoxins during cocoa processing in Bahia (BR), and verified that, despite the high occurrence of aflatoxigenic fungi (A. flavus and A. parasiticus), the levels of aflatoxins found in the samples are low, mainly in the fermentation stage, suggesting that compounds with antitoxigenic properties may be present in cocoa (Adhikari et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Filamentous fungi diversity in the natural fermentation of Amazonian cocoa beans and the microbial enzyme activities

et al. 2019

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Purpose The purpose of this study was to investigate the diversity of filamentous fungi and the hydrolytic potential of their enzymes for a future understanding of the influence of these factors on the sensory characteristics of the cocoa beans used to obtain chocolate. Methods Filamentous fungi were isolated from the natural cocoa fermentation boxes in the municipality of Tucuman, Pará, Brazil, and evaluated for the potential production of amylases, cellulases, pectinases, and xylanases. The fermentation was monitored by analyzing the pH and temperature. The strains were identified by sequencing the ITS1/ITS4 section of the 5.8S rDNA and partially sequencing the 18S and 28S regions, and the molecular identification was confirmed by phylogenetic reconstruction. Result The fungi isolated were comprised of three classes from the Ascomycota phylum and one class from the Basidiomycota phylum. There were found 19 different species, of this amount 16 had never been previously reported in cocoa fermentation. This fact characterizes the fermentation occurring in this municipality as having wide fungal diversity. Most of the strains isolated had the ability to secrete enzymes of interest. Cladosporium cladosporioides, Fomitopsis subtropical, Aspergillus versicolor, Penicillium pimiteouiense, Phanerochaete australis, Neonothopanus nambi, and Aspergillus parasiticus were the strains that excelled in the secretion of the following enzymes: amylase, pectinase, cellulase, and xylanase. Conclusion The presence of 16 species not yet reported in cocoa seed fermentations and their potential hydrolytic activities show a diversity of filamentous fungi in this microbial biome that needs to be better understood.

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“…To our knowledge, the genus Talaromyces has been frequently isolated from soil and root aquatic in Korea [ 50 51 ]. Figs trees are rarely found in Korea.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Paecilomyces variotii and Talaromyces amestolkiae in Korea Based on the Morphological Characteristics and Multigene Phylogenetic Analyses

2016

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During fungal diversity surveys of the order Eurotiales in Korea, two fungal strains, EML-DG33-1 and EML-NCP50, were isolated from samples of rat dung and fig tree leaf collected at a garden located in Gwangju in 2014. To complete the National Species List of Korea, it is a prerequisite to verify whether many questionable species, which were previously recorded but not confirmed, indeed present in Korea. Herein, the isolates were confirmed as undescribed species, Paecilomyces variotii and Talaromyces amestolkiae based on the combination of morphological and phylogenetic analyses of multigenes including the rDNA internal transcribed spacer, β-tubulin, and RNA polymerase II subunit 2.

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Discovery of Two New Talaromyces Species from Crop Field Soil in Korea

Cited by 8 publications

References 12 publications

Bioactive Metabolites From Acid-Tolerant Fungi in a Thai Mangrove Sediment

Bioactive Metabolites From Acid-Tolerant Fungi in a Thai Mangrove Sediment

Filamentous fungi diversity in the natural fermentation of Amazonian cocoa beans and the microbial enzyme activities

Characterization of Paecilomyces variotii and Talaromyces amestolkiae in Korea Based on the Morphological Characteristics and Multigene Phylogenetic Analyses

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