Discovery, verification, and validation of walnut protein marker peptides using LC-MS approaches

Kim, K.-W.; Kim, Y; Lee, Hana; Lee, Chaeyoon; Kim, Soo-Yeon; Oh, Sangsuk; Park, Zee‐Yong

doi:10.1016/j.foodchem.2023.136889

Cited by 2 publications

(1 citation statement)

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“…Due to significant variations in the peptides used for quantification across different studies, we prioritize peptides with a higher frequency of use during the selection process. Croote and Quake provided a comprehensive summary of characteristic peptides for major allergenic foods. , Building upon this information and the most recent literature, we carefully selected two target proteins and their corresponding 2–3 peptides for each allergenic food. − Subsequently, the candidate peptides underwent systematic evaluation for their MS signal intensity, specificity, recovery rate, and susceptibility to matrix effects. Ultimately, the two best-performing peptides for each food allergen were chosen for further method development.…”

Section: Resultsmentioning

confidence: 99%

Advancing High-Throughput MS-Based Protein Quantification: A Case Study on Quantifying 10 Major Food Allergens by LC-MS/MS Using a One-Sample Multipoint External Calibration Curve

Yang,

Lin,

Yang

et al. 2024

J. Agric. Food Chem.

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The LC-MS-based method has emerged as the preferred approach for quantifying food allergens. However, the preparation of a traditional calibration curve (MSCC) is labor-intensive and error-prone. Here, a sensitive and robust LC-MS/MS method for quantifying 10 major food allergens was developed and validated, where the one-sample multipoint external calibration curve (OSCC) was employed instead of MSCC. By employing the multiple isotopologue reaction monitoring (MIRM) technique with only one spiked level in the blank, OSCC can be effectively established. Results demonstrate that the proposed method exhibits excellent performance in selectivity, sensitivity, accuracy, and precision, comparable to that of the traditional MSCC. Additionally, this strategy allows for isotope sample dilution by monitoring the less abundant MIRM channel. Moreover, the developed method was successfully applied to investigate the contamination of 10 food allergens in commercial food products. With its high throughput and robustness, the MIRM-OSCC-LC-MS/MS methodology has many potential applications, especially in the MS-based protein quantification analysis.

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