DISCOVR strain screening pipeline – Part III: Strain evaluation in outdoor raceway ponds

Gao, Song; Edmundson, Scott; Huesemann, Michael H.; Gutknecht, Andrew; Laurens, Lieve M.L.; Wychen, Stefanie Van; Pittman, Kyle; Mattias, Greer,

doi:10.1016/j.algal.2023.102990

Cited by 7 publications

(1 citation statement)

References 63 publications

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“…Future investigations will delve into the effects of lutein under fluctuating environmental parameters. Picochlorum celeri has been studied in outdoor pond cultivation campaigns and found to be suitable mainly for warm seasons ( Huesemann et al, 2023a ; Huesemann et al, 2023b ; Gao et al, 2023 ; McGowen et al, 2023 ). While we believe the lutein-deficient (LCYe) strain generated in this study might be of value during the summer season and should be tested for outdoor cultivation, significant limitations might arise in fluctuating light and temperature conditions, and particularly in cold weather when NPQ processes might be hampered ( Manjre et al, 2022 ), making this strain not suitable for winter cultivation in cold climates.…”

Section: Discussionmentioning

confidence: 99%

Cas9 deletion of lutein biosynthesis in the marine alga Picochlorum celeri reduces photosynthetic pigments while sustaining high biomass productivity

Cano,

Krishnan,

Karns

et al. 2024

Front. Bioeng. Biotechnol.

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Domestication of algae for food and renewable biofuels remains limited by the low photosynthetic efficiencies of processes that have evolved to be competitive for optimal light capture, incentivizing the development of large antennas in light-limiting conditions, thus decreasing efficient light utilization in cultivated ponds or photobioreactors. Reducing the pigment content to improve biomass productivity has been a strategy discussed for several decades and the ability to reduce pigment significantly is now fully at hand thanks to the widespread use of genome editing tools. Picochlorum celeri is one of the fastest growing marine algae identified and holds particular promise for outdoor cultivation, especially in saline water and warm climates. We show that while chlorophyll b is essential to sustain high biomass productivities under dense cultivation, removing Picochlorum celeri’s main carotenoid, lutein, leads to a decreased total chlorophyll content, higher a/b ratio, reduced functional LHCII cross section and higher maximum quantum efficiencies at lower light intensities, resulting in an incremental increase in biomass productivity and increased PAR-to-biomass conversion efficiency. These findings further strengthen the existing strategies to improve photosynthetic efficiency and biomass production in algae.

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