Discrete Coil-Globule Transition of Large DNA Induced by Cationic Surfactant

Melnikov, Sergey M.; Sergeyev, Vladimir G.; Yoshikawa, Kenichi

doi:10.1021/ja00114a004

Cited by 386 publications

(435 citation statements)

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“…4 at 8 mM) is to be noted. We have found similar effect for the process of the compaction of DNA with polyethylene glycol, with polyarginine and also with cationic surfactant [19,29,30]. Considering that the persistence length of DNA is 500-1000 ]k, T4 DNA is regarded as the linear polymer composed with ca.…”

Section: Resultsmentioning

confidence: 56%

Diaminoalkanes with an odd number of carbon atoms induce compaction of a single double‐stranded DNA chain

Yoshikawa

1995

FEBS Letters

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~bstract We have performed direct observations on the conformational change of a single double-stranded T4 DNA molecule induced by diaminoalkanes, NH~(CH2).NH~ (n = 1 -6), by use of fluorescence microscopy. It was found that diamines with three and five methylene groups show a significant effect on the compaction of individual single DNA molecules. On the other hand, diamines with two, four and six methylene groups are not effective in the compaction of DNA. Such a drastic difference in the action of diamines suggests that not only the number of charge but also the length between the amino groups in the diamines plays an ~,,~sential role in their interactions with DNA.

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Section: Resultsmentioning

confidence: 56%

Diaminoalkanes with an odd number of carbon atoms induce compaction of a single double‐stranded DNA chain

Yoshikawa

1995

FEBS Letters

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“…The general aspects of DNA compaction by cationic surfactants have been monitored by Yoshikawa s group (6,15,16,(30)(31)(32) and others and by our group recently (11,33). Some illustrative fluorescence micrographs are presented in Figure 6.…”

Section: Dna Is Compacted By Cationic Surfactantsmentioning

confidence: 98%

DNA-lipid systems: A physical chemistry study

Dias

Antunes

Miguel

et al. 2002

Braz J Med Biol Res

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It is well known that the interaction of polyelectrolytes with oppositely charged surfactants leads to an associative phase separation; however, the phase behavior of DNA and oppositely charged surfactants is more strongly associative than observed in other systems. A precipitate is formed with very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. Techniques like phase diagram determinations, fluorescence microscopy, and ellipsometry were used to study these systems. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was also investigated. We observed that DNA compacts and adsorbs onto the surface of positively charged vesicles, and that the addition of an anionic surfactant can release DNA back into solution from a compact globular complex between DNA and the cationic surfactant. Finally, DNA interactions with polycations, chitosans with different chain lengths, were studied by fluorescence microscopy, in vivo transfection assays and cryogenic transmission electron microscopy. The general conclusion is that a chitosan effective in promoting compaction is also efficient in transfection.

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] There has been considerable interest in characterizing the nature of the fundamental interactions between cationic agents and DNA, and several general approaches have been designed to unravel such interactions. [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] One of the important driving forces in all of these systems is the long-range electrostatic interaction between molecules of opposite charge.…”

Section: Introductionmentioning

confidence: 99%

Effect of Headgroup on DNA−Cationic Surfactant Interactions

Dasgupta¹,

Das²,

Dias³

et al. 2007

J. Phys. Chem. B

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The interaction behavior of DNA with different types of hydroxylated cationic surfactants has been studied. Attention was directed to how the introduction of hydroxyl substituents at the headgroup of the cationic surfactants affects the compaction of DNA. The DNA-cationic surfactant interaction was investigated at different charge ratios by several methods like UV melting, ethidium bromide exclusion, and gel electrophoresis. Studies show that there is a discrete transition in the DNA chain from extended coils (free chain) to a compact form and that this transition does not depend substantially on the architecture of the headgroup. However, the accessibility of DNA to ethidium bromide is preserved to a significantly larger extent for the more hydrophilic surfactants. This was discussed in terms of surfactant packing. Observations are interpreted to reflect that the surfactants with more substituents have a larger headgroup and therefore form smaller micellar aggregates; these higher curvature aggregates lead to a less efficient, "patch-like" coverage of DNA. The more hydrophilic surfactants also presented a significantly lower cytotoxicity, which is important for biotechnological applications.

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Discrete Coil-Globule Transition of Large DNA Induced by Cationic Surfactant

Cited by 386 publications

References 0 publications

Diaminoalkanes with an odd number of carbon atoms induce compaction of a single double‐stranded DNA chain

Diaminoalkanes with an odd number of carbon atoms induce compaction of a single double‐stranded DNA chain

DNA-lipid systems: A physical chemistry study

Effect of Headgroup on DNA−Cationic Surfactant Interactions

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