Montenegro is a small country in Balkan Peninsula with very long tradition of grapevine growing and wine making that originate from the pre-Roman period. Dominant place in Montenegrin viticulture belongs to autochthonous grapevine varieties Vranac, Kratosija, and Krstac, while in minor part, the other varieties are presented. Among many literature sources, the oldest historical document that pointed out the importance of autochthonous varieties is The Medieval Statute of Budva from fifteenth century. In order to better present Montenegrin germplasm, this research provides an overview of literature, ampelographic, and genetic analysis on autochthonous and domesticated varieties. Achieved results showed an important breeding history of grapevine and a large number of unique DNA profiles. Montenegro has the richness of grapevine diversity that can significantly enrich the diversity of vines in Europe.