Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow

Riedel, René; Addo, Richard; Ferreira‐Gomes, Marta; Heinz, Gitta Anne; Heinrich, Frederik; Kummer, Jannis; Greiff, Victor; Schulz, Daniel; Klaeden, Cora; Cornelis, Rebecca; Menzel, Ulrike; Kröger, Stefan; Stervbo, Ulrik; Köhler, Ralf; Haftmann, Claudia; Kühnel, Silvia; Lehmann, Katrin; Maschmeyer, Patrick; McGrath, Mairi; Naundorf, Sandra; Hahne, Stefanie; Sercan, Özen; Siracusa, Francesco; Stefanowski, Jonathan; Weber, Melanie; Westendorf, Kerstin; Zimmermann, Jakob; Hauser, Anja E.; Chang, Hyun‐Dong; Mashreghi, Mir‐Farzin; Radbruch, Andreas

doi:10.1101/825224

Cited by 4 publications

(3 citation statements)

References 49 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Various organs, such as the spleen, lymph nodes, bone marrow, and blood, can be accessed, each displaying differences in antibody repertoire present in ASCs and MCs. [34][35][36] The quantity and quality of the induced repertoire in immunized animals highly depend on factors such as the chosen immunization protein (antigen), its quality, dose, administration, mouse strain, genetic model, harvest time, and immunization scheme, necessitating optimization for efficient and optimal screening 37,38 (unpublished data), but the frequency of antigen specificity in total cells is usually higher than in humans. Nevertheless, appropriately chosen conditions can lead to identifying high-affinity antibodies, as the affinity distribution tends to follow a normal distribution with outliers in the highaffinity range.…”

Section: Screening An Antibody Repertoire For Candidatessingle-cells ...mentioning

confidence: 99%

Antibodies, repertoires and microdevices in antibody discovery and characterization

Schlotheuber,

Lüchtefeld,

Eyer

2024

Lab Chip

View full text Add to dashboard Cite

show abstract

Section: Screening An Antibody Repertoire For Candidatessingle-cells ...mentioning

confidence: 99%

Antibodies, repertoires and microdevices in antibody discovery and characterization

Schlotheuber,

Lüchtefeld,

Eyer

2024

Lab Chip

View full text Add to dashboard Cite

show abstract

“…Im Knochenmark sitzen 80 Prozent der langlebigen Plasmazellen im direkten Kontakt zu VCAM1+-Stromalzellen (Abb. 1, [4] len in speziellen Nischen über längere Zeiträume persistieren [8,9]. Auch hier scheint der direkte Zellkontakt zu Stromalzellen wichtig zu sein, da sich sowohl die Gedächtnis-T-als auch die Gedächtnis-B-Zellen im direkten Kontakt mit den Stromalzellen befi nden.…”

Section: üBerlebensnische Der Plasmazellenunclassified

Plasmazellüberleben wird durch extrinsische Signale reguliert

2020

Self Cite

View full text Add to dashboard Cite

Secreted antibodies form the basis for protective vaccination, the most effective method of disease control to date. Plasma cells are the main producers of antibodies. A single plasma cell can secrete thousands of antibodies per second and thus forms the basis of humoral immunity of the body. While this function plays an important role to protect the body from recurring pathogens, autoreactive plasma cells secreting autoantibodies can lead to severe organ damage in diseases such as rheumatoid arthritis and systemic lupus erythematosus.

show abstract

“…It is made available under a preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in The copyright holder for this this version posted September 17, 2021. ; https://doi.org/10.1101/2021.09. 15.460420 doi: bioRxiv preprint Csepregi et al, The Physiological Landscape and Specificity of Antibody Repertoires However, similar to other studies across tissues in mice, they performed small-scale sequencing on specific B-cell subsets (11)(12)(13). Therefore, it remains an open question how the degree of clonal overlap across physiological compartments relates on repertoire-scale and whether each lymphoid organ represents an exclusive or redundant (class-switched IgG) antibody repertoire upon antigenic challenge.…”

mentioning

confidence: 99%

The Physiological Landscape and Specificity of Antibody Repertoires

Csepregi

Hoehn

Neumeier

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Diverse antibody repertoires spanning multiple lymphoid organs (e.g., bone marrow, spleen, lymph nodes) form the foundation of protective humoral immunity. Changes in their composition across lymphoid organs are a consequence of B-cell selection and migration events leading to a highly dynamic and unique physiological landscape of antibody repertoires upon antigenic challenge (e.g., vaccination). However, to what extent B cells encoding identical or similar antibody sequences (clones) are distributed across multiple lymphoid organs and how this is shaped by the strength of a humoral response, remains largely unexplored. Here, we performed an in-depth systems analysis of antibody repertoires across multiple distinct lymphoid organs of immunized mice, and discovered that organ-specific antibody repertoire features (e.g., germline V-gene usage and clonal expansion profiles) equilibrated upon a strong humoral response (multiple immunizations and high serum titers). This resulted in a surprisingly high degree of repertoire consolidation, characterized by highly connected and overlapping B-cell clones across multiple lymphoid organs. Finally, we revealed distinct physiological axes indicating clonal migrations and showed that antibody repertoire consolidation directly correlated with antigen-specificity. Our study uncovered how a strong humoral response resulted in a more uniform but redundant physiological landscape of antibody repertoires, indicating that increases in antibody serum titers were a result of synergistic contributions from antigen-specific B-cell clones distributed across multiple lymphoid organs. Our findings provide valuable insights for the assessment and design of vaccine strategies.

show abstract

Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow

Abstract: Here we describe tissue-resident memory B lymphocytes of spleen and bone marrow. Single cell transcriptomes and B cell receptor repertoires identify several exclusive populations of isotype-switched memory B cells (Bsm) in murine spleen and bone marrow, and one

Cited by 4 publications

References 49 publications

Antibodies, repertoires and microdevices in antibody discovery and characterization

Antibodies, repertoires and microdevices in antibody discovery and characterization

Plasmazellüberleben wird durch extrinsische Signale reguliert

The Physiological Landscape and Specificity of Antibody Repertoires

Contact Info

Product

Resources

About