Discriminating between serological responses to European-genotype live vaccine and European-genotype field strains of porcine reproductive and respiratory syndrome virus (PRRSV) by peptide ELISA

Oleksiewicz, Martin B.; Stadejek, Tomasz; Maćkiewicz, Zbigniew; Porowski, M.; Pejsak, Z.

doi:10.1016/j.jviromet.2005.05.017

Cited by 22 publications

(11 citation statements)

References 40 publications

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“…Based on PCR results alone it was not possible to determine whether the virus detected was the live vaccine or a field strain, in particular considering that studies elsewhere have detected vaccine virus in both vaccinated and non-vaccinated pigs [44]. This was observed mostly with vaccine virus related to the North American (NA) genotype [44-47]. On the other hand, only limited transmission of the European genotype vaccine virus could be demonstrated [44,48].…”

Section: Discussionmentioning

confidence: 99%

Risk factors for porcine reproductive and respiratory syndrome virus infection and resulting challenges for effective disease surveillance

et al. 2012

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BackgroundThis study aimed to identify risk factors for active porcine reproductive and respiratory syndrome virus (PRRSV) infection at farm level and to assess the probability of an infected farm being detected through passive disease surveillance in England. Data were obtained from a cross-sectional study on 147 farrow-to-finish farms conducted from April 2008 – April 2009. The risk factors for active PRRSV infection were identified using multivariable logistic regression analysis. The surveillance system was evaluated using a stochastic scenario tree model.ResultsEvidence of PRRSV circulation was confirmed on 35.1% (95%CI: 26.8-43.4) of farms in the cross sectional study, with a higher proportion of infected farms in areas with high pig density (more than 15000 pigs within 10 km radius from the farm). Farms were more likely to have active PRRSV infection if they used the live virus vaccine-Porcilis PRRS (OR=7.5, 95%CI: 2.5-22.8), were located in high pig density areas (OR=2.9, 95%CI: 1.0-8.3) or had dead pigs collected (OR=5.6, 95%CI: 1.7-18.3). Farms that weaned pigs at 28 days of age or later had lower odds of being PRRSV positive compared to those weaning at 21-27 days (OR=0.2, 95%CI: 0.1-0.7). The probability of detecting an infected farm through passive surveillance for disease was low (mode=0.074, 5th and 95th percentiles: 0.067; 0.083 respectively). In particular farms which used live virus vaccine had lower probabilities for detection compared to those which did not.ConclusionsRisk factors identified highlight the importance of biosecurity measures for the incursion of PRRSV infection. The results further indicate that a combined approach of surveillance for infection and disease diagnosis is needed to assist effective control and/or elimination of PRRSV from the pig population.

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Section: Discussionmentioning

confidence: 99%

Risk factors for porcine reproductive and respiratory syndrome virus infection and resulting challenges for effective disease surveillance

et al. 2012

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“…The test cannot differentiate between vaccination and reinfection. In addition, a large number of diagnostic serological assays have been developed over the years that utilize different viral antigens (22,34). However, serological diagnosis often is confounded by the failure to distinguish between antibody induced by infection and those arising from other means, such as maternal antibodies or vaccine-induced antibodies, although it might be possible to differentiate the serological response induced by vaccines from that caused by disease to a limited extent (7).…”

Section: Discussionmentioning

confidence: 99%

Detection of Asymptomatic Antigenemia in Pigs Infected by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by a Novel Capture Immunoassay with Monoclonal Antibodies against the Nucleocapsid Protein of PRRSV

Cai

Wang

Tse

et al. 2009

Clin Vaccine Immunol

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“…It was first described as the Lelystad (Wensvoort et al, 1991) virus in the Netherlands and VR-2332 in the U.S. The two prototype virus strains share approximately 60% nucleotide identity and have been divided into two main PRRSV genotypes: European (EU) type (genotype 1) and North American (NA) type (genotype 2) (Nelsen et al, 1999;Oleksiewicz et al, 2005).…”

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confidence: 99%

Development and validation of a recombinant nucleocapsid protein-based ELISA for detection of the antibody to porcine reproductive and respiratory syndrome virus

Chu

Kim

et al. 2009

J Microbiol.

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Three indirect enzyme-linked immunosorbent assays (iELISA) based on the North American like (NA-like), European like (EU-like) and co-expressed NA- and EU-like recombinant nucleocapsid proteins (N-protein) of porcine reproductive and respiratory syndrome virus (PRRSV) were validated for the detection of the antibodies in porcine sera. A total of 422 serum samples from unvaccinated pigs were tested. The cut-off value was optimized by a two-graph receiver operating characteristics analysis at a 95% confidence level. This assay was validated with Western blot analysis and IDEXX HerdChek ELISA. Cross-reactivity results showed that iELISA was PRRSV-specific. Repeatability tests revealed that the coefficients of variation of positive sera within and between runs were less than 10%. The results indicate that iELISA is simpler to produce and perform, time-saving and suitable for large scale surveys of PRRSV infection at low cost, and is potentially useful to evaluate the efficiency of various vaccines against PRRSV.

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Discriminating between serological responses to European-genotype live vaccine and European-genotype field strains of porcine reproductive and respiratory syndrome virus (PRRSV) by peptide ELISA

Cited by 22 publications

References 40 publications

Risk factors for porcine reproductive and respiratory syndrome virus infection and resulting challenges for effective disease surveillance

Risk factors for porcine reproductive and respiratory syndrome virus infection and resulting challenges for effective disease surveillance

Detection of Asymptomatic Antigenemia in Pigs Infected by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by a Novel Capture Immunoassay with Monoclonal Antibodies against the Nucleocapsid Protein of PRRSV

Development and validation of a recombinant nucleocapsid protein-based ELISA for detection of the antibody to porcine reproductive and respiratory syndrome virus

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