Discriminating Foot-and-Mouth Disease Virus-Infected and Vaccinated Animals by Use of β-Galactosidase Allosteric Biosensors

Sánchez-Aparicio, María Teresa; Rosas, María F.; Ferraz, Rosa María; Delgui, Laura Ruth; Veloso, J. J.; Blanco, Esther; Villaverde, Antonio; Sánchez‐Madrid, Francisco

doi:10.1128/cvi.00139-09

Cited by 7 publications

(6 citation statements)

References 47 publications

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“…Among the advantages of such biosensors as diagnostic tools is the homogeneous nature of the assay, the short time required for the enzymatic reaction and antibody detection, and the potential for handling large number of samples and for automatic processing, as shown for human immunodeficiency virus (Ferraz et al, 2006;Ferraz et al, 2008). More recently, allosteric biosensors for detection of the animal foot-and-mouth disease virus (FMDV) have been developed, allowing the differentiation between sera FMDV-infected animals and those of naïve and conventionally vaccinated ones (Sánchez-Aparicio et al, 2009).…”

Section: Biosensors and Fusion Proteinsmentioning

confidence: 99%

Recombinant microbial systems for improved β-galactosidase production and biotechnological applications

Oliveira

Guimarães

Domingues

2011

Biotechnology Advances

153

105

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β-Galactosidases (EC 3.2.1.23) constitute a large family of proteins that are known to catalyze both hydrolytic and transgalactosylation reactions. The hydrolytic activity has been applied in the food industry for decades for reducing the lactose content in milk, while the transgalactosylation activity has been used to synthesize galacto-oligosaccharides and galactose containing chemicals in recent years. The main focus of this review is on the expression and production of Aspergillus niger, Kluyveromyces lactis and bacterial β-galactosidases in different microbial hosts. Furthermore, emphasis is given on the reported applications of the recombinant enzymes. Current developments on novel β-galactosidases, derived from newly identified microbial sources or by protein engineering means, together with the use of efficient recombinant microbial production systems are converting this enzyme into a relevant synthetic tool. Thermostable β-galactosidases (cold-adapted or thermophilic) in addition to the growing market for functional foods will likely redouble its industrial interest.

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Section: Biosensors and Fusion Proteinsmentioning

confidence: 99%

Recombinant microbial systems for improved β-galactosidase production and biotechnological applications

Oliveira

Guimarães

Domingues

2011

Biotechnology Advances

153

105

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“…(4) Novel Diagnostic Assays. Additional novel diagnostic assays such as biosensors [ 85 ], microarrays [ 86 ], gold nanoparticle (GNP) improved immuno-PCR (GNP-IPCR ) [ 87 ], and nucleic acid sequence based amplification (NASBA) [ 88 ] have shown promising ability for rapid and reliable diagnosis, surveillance screening, and strain typing for FMDV for sub-Saharan Africa.Some of the limitations of these assays are that they are yet to beoptimised for the FMDV SAT serotypes with a high degree of sequence variability like Zambia. The costs involved per test will also determine how widely these assays will be used especially for developing countries like Zambia.…”

Section: Challenges Of Controlling Fmd In Zambiamentioning

confidence: 99%

Challenges and Economic Implications in the Control of Foot and Mouth Disease in Sub-Saharan Africa: Lessons from the Zambian Experience

Sinkala

Simuunza

Pfeiffer

et al. 2014

Veterinary Medicine International

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Foot and mouth disease is one of the world's most important livestock diseases for trade. FMD infections are complex in nature and there are many epidemiological factors needing clarification. Key questions relate to the control challenges and economic impact of the disease for resource-poor FMD endemic countries like Zambia. A review of the control challenges and economic impact of FMD outbreaks in Zambia was made. Information was collected from peer-reviewed journals articles, conference proceedings, unpublished scientific reports, and personal communication with scientists and personal field experiences. The challenges of controlling FMD using mainly vaccination and movement control are discussed. Impacts include losses in income of over US$ 1.6 billion from exports of beef and sable antelopes and an annual cost of over US$ 2.7 million on preventive measures. Further impacts included unquantified losses in production and low investment in agriculture resulting in slow economic growth. FMD persistence may be a result of inadequate epidemiological understanding of the disease and ineffectiveness of the control measures that are being applied. The identified gaps may be considered in the annual appraisal of the FMD national control strategy in order to advance on the progressive control pathway.

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“…Additional novel diagnostic assays such as biosensors, 202 microarrays, 203 gold nanoparticle improved immuno-PCR, 204 and nucleic acid sequence-based amplification 205 , 206 have been shown to enable rapid and reliable diagnosis, surveillance screening, and strain typing for FMDV. Although these assays have promising capabilities for sub-Saharan Africa and can improve many of the current problems faced, there are also many limiting factors that prevent the routine use of certain assays.…”

Section: Current and Novel Diagnostic Tests For Field Application In mentioning

confidence: 99%

“…These were consistent within serotypes, and were found to match some of the independently identified antigenic sites in other serotypes. Applying the linear mixed-effect model to SAT1 viruses, two surface-exposed regions of the capsid (ie, VP1 βG-βH (132-174) and VP3 βH-βI (191)(192)(193)(194)(195)(196)(197)(198)(199)(200)(201)(202) loops were identified as better predictors of cross-reactivity between the field virus and vaccine strain 66 than serologically calculated r 1 -values. Both regions were identified by studying MAbresistant mutant viruses.…”

mentioning

confidence: 99%

Challenges and prospects for the control of foot-and-mouth disease: an African perspective

Maree¹,

Kasanga²,

Scott³

et al. 2014

VMRR

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Abstract:The epidemiology of foot-and-mouth disease (FMD) in Africa is unique in the sense that six of the seven serotypes of FMD viruses (Southern African Territories [SAT] 1, SAT2, SAT3, A, O, and C), with the exception of Asia-1, have occurred in the last decade. Due to underreporting of FMD, the current strains circulating throughout sub-Saharan Africa are in many cases unknown. For SAT1, SAT2, and serotype A viruses, the genetic diversity is reflected in antigenic variation, and indications are that vaccine strains may be needed for each topotype. This has serious implications for control using vaccines and for choice of strains to include in regional antigen banks. The epidemiology is further complicated by the fact that SAT1, SAT2, and SAT3 viruses are maintained and spread by wildlife, persistently infecting African buffalo in particular. Although the precise mechanism of transmission of FMD from buffalo to cattle is not well understood, it is facilitated by direct contact between these two species.Once cattle are infected they may maintain SAT infections without the further involvement of buffalo. No single strategy for control of FMD in Africa is applicable. Decision on the most effective regional control strategy should focus on an ecosystem approach, identification of primary endemic areas, animal husbandry practices, climate, and animal movement. Within each ecosystem, human behavior could be integrated in disease control planning. Different regions in sub-Saharan Africa are at different developmental stages and are thus facing unique challenges and priorities in terms of veterinary disease control. Many science-based options targeting improved vaccinology, diagnostics, and other control measures have been described. This review therefore aims to emphasize, on one hand, the progress that has been achieved in the development of new technologies, including research towards improved tailored vaccines, appropriate vaccine strain selection, vaccine potency, and diagnostics, and how it relates to the conditions in Africa. On the other hand, we focus on the unique epidemiological, ecological, livestock farming and marketing, socioeconomic, and governance issues that constrain effective FMD control. Any such new technologies should have the availability of safe livestock products for trade as the ultimate goal.

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Discriminating Foot-and-Mouth Disease Virus-Infected and Vaccinated Animals by Use of β-Galactosidase Allosteric Biosensors

Cited by 7 publications

References 47 publications

Recombinant microbial systems for improved β-galactosidase production and biotechnological applications

Recombinant microbial systems for improved β-galactosidase production and biotechnological applications

Challenges and Economic Implications in the Control of Foot and Mouth Disease in Sub-Saharan Africa: Lessons from the Zambian Experience

Challenges and prospects for the control of foot-and-mouth disease: an African perspective

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