Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers

Mailer, Rodney J.; Scarth, R.; Fristensky, Brian

doi:10.1007/bf00222895

Cited by 108 publications

(67 citation statements)

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“…However, phenotype is influenced by the environment and its interaction with a particular genotype. Molecular DNA techniques such as random amplified polymorphic DNA (RAPD) analysis have been applied to detect genetic variation in various crops (Mailer et al 1994;Demeke et al 1996). RAPD analysis has the advantage of identifying polymorphisms independent of both prior DNA sequence knowledge and environmental influence.…”

Section: Mots Clésmentioning

confidence: 99%

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Utilization of doubled haploid technique in Brassica rapa population improvement

Friesen¹,

Scarth²

2000

Can. J. Plant Sci.

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R. 2000. Utilization of doubled haploid technique in Brassica rapa population improvement. Can. J. Plant Sci. 80: 75-82. The utilization of doubled haploid (DH) plants in population improvement of Brassica rapa was studied by randomly intercrossing 4, 8, 12 and 22 DH lines developed from the B. rapa cultivar Reward and the B. rapa breeding line DSC-3 for two generations to constitute synthetic populations. The synthetic populations and the DH plants used in their formation were evaluated for agronomic performance at two locations in the field in 1996 and for genetic variation using random amplified polymorphic DNA (RAPD) analysis. Intercrossing as few as four DH lines from the breeding line DSC-3 produced a synthetic population with improved performance over that of the contributing DH lines. The synthetic population produced from the interpollination of eight DH lines showed an agronomic performance over that of the contributing DH lines to a level similar to the Reward donor population. RAPD analysis efficiently characterized the genotypic variation present in DH lines and synthetic populations, detecting 22-72% polymorphism between DH lines, 17-53% and 27-47% polymorphism in the first and second synthetic populations, respectively. This characterization may be useful as a tool in the reestablishment of heterogeneity and recovery of agronomic performance in B. rapa synthetic populations derived from DH lines by determining the level of genetic variability among DH lines and therefore optimal population size. Ces populations, et les plantes DH utilisées dans leur formation, ont ensuite été évaluées sur leurs performances agronomiques à deux emplacements au champ en 1996, puis sur la variabilité génétique par l'analyse RAPD. En intercroisant aussi peu que 4 lignées DH issues de DSC-3, on a produit une population synthétique démontrant de meilleures performances que les lignées DH parentales. L'amélioration, par rapport aux lignées de départ, des performances agronomiques de la population synthétique issue de l'interpollinisation de 8 lignées DH était comparable à celle observée dans la population originale du cultivar Reward. L'analyse RAPD a permis de caractériser la variation génotypique au sein des lignées DH et des populations synthétiques, détectant 22 à 72 % de polymorphisme parmi les lignées DH et 17 à 53 et 27 à 47 %, respectivement, dans les populations synthétiques de première et de seconde génération. Cette caractérisation peut être utilisée comme moyen de rétablir l'hétérogénéité et de relever les performances agronomiques des populations synthétiques de B. rapa issues de lignées DH, en déterminant le niveau de variabilité géné-tique parmi ces lignées et, par voie de conséquence la taille optimale de la population. Mots clés:Brassica rapa, population synthétique, dihaploïdes, RAPD, performances agronomiques Until 1996, the canola acreage in western Canada was divided approximately equally between the two species Brassica napus and B. rapa, with the latter occupying over 80% of the northern canola gr...

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Section: Mots Clésmentioning

confidence: 99%

“…PCR was performed using a PTC-100™ (MJ Research, Inc.), as reported by Mailer et al (1994). RAPD products were separated by electrophoresis on ethidium bromide stained 1.4% agarose gels in 1× TAE buffer.…”

Section: Rapd Analysismentioning

confidence: 99%

Utilization of doubled haploid technique in Brassica rapa population improvement

Friesen¹,

Scarth²

2000

Can. J. Plant Sci.

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“…Molecular markers have successfully been applied in registration activities of cultivars (Mailer et al, 1994) and also identification of varieties as a part of seed and grain trade (Bligh et al, 1999). A powerful technique for DNA fingerprinting is the Polymerase Chain Reaction (PCR) amplification of tandem repeat sequences.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Genetic Diversity of Aman Rice (Oryza sativa L.) Landraces in Bangladesh

Siddique¹,

Khalequzzaman²,

Fatema³

et al. 2017

Bangladesh Rice J.

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Assessment of genetic diversity and molecular characterization among rice landraces of Bangladesh is important for their identification. Genetic diversity of 96 Aman (rainfed, partially irrigated) rice landraces of Bangladesh were evaluated using eight SSR markers to characterize the landraces and also to establish the sovereignty of Bangladeshi rice gene pool. A total of 159 alleles were detected. The number of alleles per locus ranged from 13 (RM60, RM237) to 34 (RM163), with an average of 19.88. The polymorphism information content (PIC) which ranged from 0.86 (RM237) to 0.95 (RM163) with an average of 0.90 revealed much variation among the studied landraces. RM163 was the best marker for identification and diversity estimation of Aman rice landraces as revealed by PIC values. The allele frequency ranged from 8.33% (RM163) to 22.92% (RM60, RM125) with an average of 15.89. The UPGMA dendrogram based on Nei's genetic distance revealed seven distinct clusters with a similarity coefficient of 0.09. The two-dimensional graphical view of Principal Coordinate Analysis (PCoA) revealed that the landraces Pankaij, Lotha, Chinigura, Patjag, Chinikanai, Badshabogh, Panati, Jirasail, Joria, Dudhmoni, Jhingasail were found far away and distributed around the centroid of the cluster. The findings of this study are useful for landraces identification to assist plant breeders in selecting suitable genetically diverse parents for breeding programmes.

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“…Hence, the proposed plant variety protection lends added urgency to the search for solutions to the conservation of plant genetic diversity. Molecular markers have been successfully applied in registration activities including cultivar identification (Mailer et al, 1994). However, with the necessity of varietal identity, present study was an attempt to reveal genetic diversity and varietal identification of eight cotton varieties in Bangladesh by DNA fingerprinting using microsatellite markers.…”

Section: Analysis Of Microsatellite Datamentioning

confidence: 99%

DNA Fingerprinting and Genotyping of Cotton Varieties Using SSR Markers

Islam

Molla

Rohman

et al. 2012

Not Bot Hort Agrobot Cluj

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DNA fingerprinting and genetic diversity analysis helps direct selective breeding and conservation of plant species. Since simple sequence repeats (SSR) markers are co-dominant, they can predict level of genetic diversity and thereby protect plant genetic resources of a region. Keeping the aforesaid rationale in mind, we worked on molecular characterization of eight cotton varieties in Bangladesh using simple sequence repeat (SSR) or microsatellite DNA markers. All the three microsatellite DNA markers were found to be polymorphic, extracting a total of eight alleles with an average of 2.67 alleles per locus in the present study. Allele sizes were as 149-155 bp, 178-198 bp and 140-202 bp for the loci BNL1551, BNL1721 and BNL2960, respectively. Polymorphism Information Content (PIC) values were ranged from 0.469 to 0.531. UPGMA dendrogram separated 8 varieties of cotton into two clusters. One cluster contained six varieties CB-1, CB-2, CB-3, CB-7, CB-9 and CB-10 while other two varieties CB-5 and HC 1 formed another cluster. The findings of this study would provide a useful guide for selecting specific germplasm with distinct genetic background for diversifying cotton breeding program in Bangladesh.

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Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers

Cited by 108 publications

References 11 publications

Utilization of doubled haploid technique in Brassica rapa population improvement

Utilization of doubled haploid technique in Brassica rapa population improvement

Molecular Characterization and Genetic Diversity of Aman Rice (Oryza sativa L.) Landraces in Bangladesh

DNA Fingerprinting and Genotyping of Cotton Varieties Using SSR Markers

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