Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer

Xi, Fengna; Gao, Jingqing; Wang, Jine; Wang, Zhenxin

doi:10.1016/j.jelechem.2010.10.025

Cited by 40 publications

(27 citation statements)

References 23 publications

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“…The CramoLL-bacterial LPS binding process may be related to the carbohydrates, glucose and mannose, imparting a LPS sensing mechanism on the CramoLL interface, a process similar to that observed for lectin ConA [35]. Experimental results demonstrate that the S. marcescens LPS interacts with the CramoLL system through the recognition of CramoLL with the glycosyl and mannosyl residues on the LPS structure.…”

Section: Properties Of Cramoll Lectin For Lps Recognitionmentioning

confidence: 64%

Impedimetric biosensor based on self-assembled hybrid cystein-gold nanoparticles and CramoLL lectin for bacterial lipopolysaccharide recognition

Oliveira

Andrade

Correia

et al. 2011

Journal of Colloid and Interface Science

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We report the development of a new selective and specific electrochemical biosensor for bacterial lipolysaccharide (LPS). An electrode interface was constructed using a l-cysteine-gold nanoparticle (AuNpCys) composite to be immobilized by electrostatic interaction in the network of a poly(vinyl chloride-vinyl acetate maleic acid) (PVM) layer on a gold bare electrode. The impedimetric biosensor is fabricated by self-assembled CramoLL lectin on the PVM-AuNpCys-modified gold electrode through electrostatic interaction. CramoLL is used as the recognition interface. AFM images showed that LPS was specifically recognized on the PVM-AuNpCys-CramoLL system surface. The measurements of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) showed that the electrochemical response of a redox probe system (K(4)[Fe(CN)(6)](4-)/K(3)[Fe(CN)(6)](3-)) were blocked, due to the procedures of modified electrode with PVM-AuNpCys-CramoLL. In the majority of the experiments the lectin retained its activity as observed through its interaction with LPS from Escherichia coli, Serratia marcescens, Salmonella enterica and Klebsiella pneumoniae. The results are expressed in terms of the charge transfer resistance and current peak anodic using the EIS and CV techniques for the development of a biosensor for contamination by endotoxins. A new type of sensor for selective discrimination of LPS types with a high sensitivity has been obtained.

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Section: Properties Of Cramoll Lectin For Lps Recognitionmentioning

confidence: 64%

Impedimetric biosensor based on self-assembled hybrid cystein-gold nanoparticles and CramoLL lectin for bacterial lipopolysaccharide recognition

Oliveira

Andrade

Correia

et al. 2011

Journal of Colloid and Interface Science

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“…Similarly, this group reported a biosensor based on Fe 3 O 4 NPs and CramoLL lectin as an electrochemical dengue serotypes biosensor [106]. In addition, Xi et al reported an impedimetric biosensor fabricated by simply and reproducibly self-assembling a lectin monolayer on the polyelectrolyte modified electrode through electrostatic interactions [107].…”

Section: Lectin-based Biosensors For Detecting Carbohydratesmentioning

confidence: 96%

“…The significant increase in the sensitivity and specificity of bacterial detection was owing to two factors: the oriented immobilization of Con A enables high affinity binding with LPS O-antigen and the dual carbohydrate-lectincarbohydrate recognition increase the specificity of the detection. Table 2 summarizes several examples of the lectin-based biosensors for clinical diagnostic of cancer [107] as well as bacterial and viral infection [114]. Lectin arrays for profiling cell surface carbohydrate expression using Con A, wheat germ agglutinin (WGA), Ulex europaeus agglutinin I (UEA), Ricinus communis agglutinin (RCA), Griffonia simplificolia lectin II, and Sambucus nigra agglutinin (SNA) deposited on gold chips [115] were developed and used to analyze cell surface carbohydrate expression via fluorescence.…”

Section: Lectin-based Biosensors For Detecting Carbohydratesmentioning

confidence: 99%

Carbohydrate–protein interactions and their biosensing applications

Zeng

Andrade²,

Oliveira³

et al. 2011

Anal Bioanal Chem

105

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Carbohydrate recognition is clearly present throughout nature, playing a major role in the initial attachment of one biological entity to another. The important question is whether these prevalent interactions could provide a real suitable alternative to the use of antibodies or nucleic acid for detection and identification. Currently, examples of carbohydrates being employed in biological detection systems are limited. The challenges of using carbohydrate recognition for detection mainly come from the weak affinity of carbohydrate-protein interactions, the lack of versatile carbohydrate scaffolds with well-defined structures, and the less developed high-information-content, real-time, and label-free assay technology. In this review, we focus on discussing the characteristics of carbohydrate-protein interactions in nature and the methods for carbohydrate immobilization based on surface coupling chemistry in terms of their general applicability for developing carbohydrate- and lectin-based label-free sensors. Furthermore, examples of innovative design of multivalent carbohydrate-protein interactions for sensor applications are given. We limit our review to show the feasibility of carbohydrate and lectin as recognition elements for label-free sensor development in several representative cases to formulate a flexible platform for their use as recognition elements for real-world biosensor applications.

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“…They used multiple biocatalytic signal amplification protocol (C 60 , Au-SiO2, and avidin-biotin system,) thus leads to 2 orders of magnitude improvement insensitivity for bacteria detection, the but the technology was more complicated and the number of process steps that were needed was higher. 15 were not in use, they were stored at 4 °C. After storing for 28 days, the response changed less than 3.9% compared with the initial steady state value.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Currently, amperometric immunosensor has been widely studied for pathogen detection with convenience and simple preparation [12][13][14][15]. To construct an useful electrochemical immunosensor, working electrode should have large surface areas to load capture molecules, good biocompatibility to preservate biological activity of bimolecular and excellent conductivity to efficiently transport electron [16].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical immunosensor for Enterobacter sakazakii detection based on electrochemically reduced graphene oxide–gold nanoparticle/ionic liquid modified electrode

Dou

Zhao

2015

Journal of Electroanalytical Chemistry

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Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer

Cited by 40 publications

References 23 publications

Impedimetric biosensor based on self-assembled hybrid cystein-gold nanoparticles and CramoLL lectin for bacterial lipopolysaccharide recognition

Impedimetric biosensor based on self-assembled hybrid cystein-gold nanoparticles and CramoLL lectin for bacterial lipopolysaccharide recognition

Carbohydrate–protein interactions and their biosensing applications

Electrochemical immunosensor for Enterobacter sakazakii detection based on electrochemically reduced graphene oxide–gold nanoparticle/ionic liquid modified electrode

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