Discrimination and highly selective adsorption of phosphoproteins and glycoproteins with arginine-functionalized polyhedral oligomeric silsesquioxane frameworks

Abstract

The combination of salt-templated thermal polymerization with arginine functionalization produces a porous POSS framework, which facilitates discrimination of phosphoproteins and glycoproteins.

Bifunctional Magnetic Supramolecular-Organic Framework: A Nanoprobe for Simultaneous Enrichment of Glycosylated and Phosphorylated Peptides

“…The simultaneous recognition of glycosylated and phosphorylated proteins plays a significant role in several cellular activities but receives much less research interests. Only a few materials have been applied in this field, such as chitosan nanomaterial, magnetic titania, , titanium nanoprobe, magnetic graphene, polyhedral oligomeric silsesquioxane, metal–organic frameworks (MOFs), and their derivatives. − Among these materials, MOFs with combined properties of HILIC and IMAC/MOAC have attracted much attention due to their unique properties including intrinsic porosity, high surface area, and abundant binding sites. However, it is rather difficult to develop reliable and reproducible methods to achieve robust MOFs with tailored structures.…”

Titanium dioxide‐functionalized dendritic mesoporous silica nanoparticles for highly selective isolation of phosphoproteins

“…As shown in the SDS‐PAGE results (Fig. 5), the protein bands identified in the original diluted defatted milk (Lane 2) are mainly ascribed to phosphoproteins (α‐casein [α‐ca] and β‐ca) and nonphosphoproteins (BSA, β‐lactoglobulin (β‐Lg) and α‐lactalbumin [α‐La]) [10, 46]. After treatment with DMSNs‐TiO 2 , a distinct decrease in the bands of α‐ca and β‐ca are observed, while the other coexisting nonphosphoproteins bands exhibit no obvious changes (Lane 3).…”

“…Hydration ions form between water molecules and inorganic ions, which remove water molecules around phosphoproteins, leading to loose state of protein structures [45] and expose partially buried phosphate groups and, thus, a slight increase in adsorption efficiency is observed. Considering salt is generally present in CeO 2 microspheres 82.4 [20] Fe 3 O 4 @fTiO 2 141.4 [22] PP-1-Arg 31.5 [46] Dye-affinity hollow fibers 80.7 [47] Fe 3 O 4 @SiO 2 @GDN 59.6 [48] DMSNs-TiO 2 157.2 This work the actual sample, 10 mmol/L glycine buffer at pH 2 without NaCl is used to prepare protein solutions. To obtain the adsorption isotherm of β-ca by DMSNs-TiO 2 , 0.1 mg of DMSNs-TiO 2 is used for the adsorption of β-ca within a concentration range of 27-225 mg/L at room temperature.…”

“…5), the protein bands identified in the original diluted defatted milk (Lane 2) are mainly ascribed to phosphoproteins (α-casein [α-ca] and β-ca) and nonphosphoproteins (BSA, β-lactoglobulin (β-Lg) and α-F I G U R E 5 SDS-PAGE assay results of defatted milk. Lane 1: protein marker (kDa); lane 2: defatted milk after diluted for 25-fold; lane 3: the supernatant of 25-fold diluted defatted milk after treatment with DMSNs-TiO 2 ; lane 4: the eluent; lane 5: standard β-ca of 150 mg/L lactalbumin [α-La]) [10,46]. After treatment with DMSNs-TiO 2 , a distinct decrease in the bands of α-ca and β-ca are observed, while the other coexisting nonphosphoproteins bands exhibit no obvious changes (Lane 3).…”

Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment

“…To mimic the multiple hydrogen bonds in nature, magnetic nanospheres were grafted with polymer brushes containing abundant grandly groups [23]. The novel material possesses the specific capture capacity of phosphoproteins rely on the more stable and more extensive functional affinity sites [23,24]. Although the progress has been made in the enrichment materials of phosphoproteins, the gap between artificial polymer and native ones needs to be filled.…”