Discrimination of Acacia gums by MALDI-TOF MS: applications to micro-samples from works of art

Granzotto, Clara; Sutherland, Ken; Arslanoglu, Julie; Ferguson, G. A.

doi:10.1016/j.microc.2018.08.058

Cited by 21 publications

(22 citation statements)

References 32 publications

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“…[25] assessed the influence of pigments and protein aging on protein identification in casein‐based paints by using ELISA. The same authors [26] were able to discriminate Acacia gums from the two most widely used and commercially significant species, A. Senegal and A. seyal via MALDI‐TOF MS. This is important because these gums are widely used in art objects, from ancient Egypt to 20th century painting (e.g., by Georges Braque).…”

Section: Discussionmentioning

confidence: 99%

“1984”: What Orwell could not predict. Proteomic analysis of his scripts

Saravayskaya

Zilberstein

et al. 2020

Electrophoresis

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George Orwell, fighter for the Republican Army during the Spanish Civil War, was shot through the throat by a sniper on 20th May 1937 and nearly killed. After receiving only a summary external treatment, on the 29th, he was cured in a Barcelona hospital where he was infected by the Koch bacillus. After fleeing from Spain on 23rd June 1937, he repaired to his cottage in Wallington, Hertfordshire, wherefrom he wrote a letter to Sergey Dynamov, Editor of Soviet journal “Foreign Literature.” This typewritten letter was analyzed by application of five EVA strips (ethylene vinyl acetate studded with strong cation and anion and with C8 and C18 resins; four on the corners and one over his signature), searching for biological traces. Upon elution of the captured biologicals, trypsin digestion and Orbitrap Fusion trihybrid mass spectrometer analyses, three of the five strips yielded clear traces of six unique proteins (via proteotypic peptides) of the tuberculosis bacterium. Additionally, MALDI TOF analysis of saliva of a tuberculosis patient and the EVA strip eluates gave a spectrum of 14 peptide bands (Mr 2700 to 6700 Da range) coincident between the two samples, thus, fully confirming Orwell's pathology. These results are attributed to saliva traces on Orwell's fingertips and to the fact that the letter was written on 2nd July 1937, when Orwell's pathology was at its peak.

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Section: Discussionmentioning

confidence: 99%

“1984”: What Orwell could not predict. Proteomic analysis of his scripts

Saravayskaya

Zilberstein

et al. 2020

Electrophoresis

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“…Ren et al 47 assessed the influence of pigments and protein aging on protein identification in casein-based paints by using enzyme-linked immunosorbent assay. The same authors 48 were able to discriminate Acacia gums from the two most widely used and commercially significant species, A. senegal and A. seyal via MALDI-TOF MS. This is important because these gums are widely used in art objects, from ancient Egypt to 20th century paintings (e.g., by Georges Braque).…”

Section: ■ Pitfalls and Limitationsmentioning

confidence: 94%

EVA Technology and Proteomics: A Two-Pronged Attack on Cultural Heritage

Righetti

Zilberstein²,

Zilberstein³

2020

J. Proteome Res.

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A novel way for exploring the world’s cultural heritage in the absence of damage or contamination (such as removing pigments in paintings or chipping away pieces of bones) of the items under investigation is here reported, called the EVA technique. It is based on films of ethylene vinyl acetate (EVA) impregnated with strong anion and cation exchangers, admixed with hydrophobic resins, C8 and C18. When in contact with any surface these films can harvest nanomoles of macromolecules (proteins and DNA) as well as metabolites, which can then be identified by standard instrumentation. Some important applications are reported, such as the findings of the renal pathology and assumption of morphine in the original manuscript of Master I Margarita by Bulgakov, the presence of TBC bacterium in Chekhov’s shirt and in a letter by Orwell, the Y. pestis and anthrax bacteria in the death registries of Milan’s lazaretto in the 1630 plague bout, as well as ample traces of five metals in Kepler’s manuscripts, suggesting his potential practice of alchemy. Also, in the pages of the Memoirs of Casanova, although the gonorrhea bacterium could not be found, spots of HgS could be measured, suggesting its use for curing the disease. A family of EVA films is described, enlarging its use to dedicated applications, such as the capture of drugs of abuse in the pages of famous writers and even in the paintings of fauvists. It is hoped that the present methodology could open the doors of museums, state archives, and private collections for detecting biological traces left by artists, literates, and men of culture in their masterpieces.

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“…To unravel these constraints and larger contexts, adhesive finds first must be characterized. Methods like ancient DNA analysis and enzymatic digestion of plant gum sugars coupled to MALDI-TOF MS and MS/MS analysis may further hone the characterization of adhesive materials and their makers (Granzotto et al 2019;Jensen et al 2019). The chemical make-up (GC-MS) and crystalline structures (spectroscopy) of adhesives are imperative for the identification of ingredients and their sources.…”

Section: What Adhesives Revealmentioning

confidence: 99%

Archaeological Adhesives

Langejans¹,

Aleo²,

Fajardo³

et al. 2022

Oxford Research Encyclopedia of Anthropology

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An adhesive is any substance that bonds different materials together. This broad definition includes materials used in everything from hafted stone tools to monumental architecture. In addition, the combination of bonding, plasticity, and insolubility meant that some adhesives were exploited for waterproofing and sealing of materials, as self-adhering inlays and putties, and as paints, varnishes, and inks. Adhesives have a history of at least 200,000 years. Throughout (pre)history and around the world, people used materials, including bitumen/asphalt, carbohydrate polymers such as starches and gums, natural rubbers, mortars, proteins (from casein, soy, blood, and animal connective tissue), insect and plant resins, and tars made from various barks and woods. Adhesives thus are very diverse and have widely varying properties: they can be tacky, pliable, elastic, brittle, water-resistant, fluid, viscous, clear, dark, and much more. They are a plastic avant la lettre. These properties can and were tweaked by mixing ingredients or by further processing. In the study of archaeological adhesives, their characterization is essential and this is best done with chemical and spectroscopic methods. When larger coherent samples as opposed to single finds are analyzed, adhesive studies can provide data on past technologies, socioeconomic organizations, and environments and raw material availability. Through sourcing and mapping of ingredients and adhesive end products, travel and transfer of materials and knowledge can be illuminated. Additionally, experimental reproductions provide data on technological aspects that otherwise are lost in the archaeological record. An archaeology of adhesives can reveal the transport networks, subsistence, mobility strategies, division of labor, and technological know-how that held societies together.

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Discrimination of Acacia gums by MALDI-TOF MS: applications to micro-samples from works of art

Cited by 21 publications

References 32 publications

“1984”: What Orwell could not predict. Proteomic analysis of his scripts

“1984”: What Orwell could not predict. Proteomic analysis of his scripts

EVA Technology and Proteomics: A Two-Pronged Attack on Cultural Heritage

Archaeological Adhesives

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