Discrimination of Alicyclobacillus Strains by Lipase and Esterase Fingerprints

Cai, Rui; Yuan, Yahong; Wang, Zhouli; Wang, Jun; Yue, Tianli

doi:10.1007/s12161-015-0290-8

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…reactivity (19,20,23,75), fragment-based inhibitor design analogous to serine protease fluorogenic SAS libraries (37,39,40), and in vivo imaging of cell type-specific hydrolase activity (24,33).…”

Section: Molecular Fluorogenic Sar Librarymentioning

confidence: 99%

“…The wide range of substrates and reactivities present among esterases has necessitated the development of diverse substrate analogs and assays for characterizing in vitro and in vivo esterase activity (15)(16)(17)(18)(19)(20)(21)(22). Esterase substrate libraries have been used to rapidly fingerprint and classify various bacterial, fungal, and disease states (19,20,(23)(24)(25)(26)(27)(28). High rates of background hydrolysis, however, limit the cellular and high-throughput screening utility of many commonly employed substrates (16,22,25,28).…”

mentioning

confidence: 99%

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Fluorogenic structure activity library pinpoints molecular variations in substrate specificity of structurally homologous esterases

White

Koelper

Russell

et al. 2018

Journal of Biological Chemistry

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Cellular esterases catalyze many essential biological functions by performing hydrolysis reactions on diverse substrates. The promiscuity of esterases complicates assignment of their substrate preferences and biological functions. To identify universal factors controlling esterase substrate recognition, we designed a 32-member structure-activity relationship (SAR) library of fluorogenic ester substrates and used this library to systematically interrogate esterase preference for chain length, branching patterns, and polarity to differentiate common classes of esterase substrates. Two structurally homologous bacterial esterases were screened against this library, refining their previously broad overlapping substrate specificity. esterase ybfF displayed a preference for γ-position thioethers and ethers, whereas Rv0045c from displayed a preference for branched substrates with and without thioethers. We determined that this substrate differentiation was partially controlled by individual substrate selectivity residues Tyr-119 in ybfF and His-187 in Rv0045c; reciprocal substitution of these residues shifted each esterase's substrate preference. This work demonstrates that the selectivity of esterases is tuned based on transition state stabilization, identifies thioethers as an underutilized functional group for esterase substrates, and provides a rapid method for differentiating structural isozymes. This SAR library could have multifaceted future applications, including imaging, biocatalyst screening, molecular fingerprinting, and inhibitor design.

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Section: Molecular Fluorogenic Sar Librarymentioning

confidence: 99%

mentioning

confidence: 99%

Fluorogenic structure activity library pinpoints molecular variations in substrate specificity of structurally homologous esterases

White

Koelper

Russell

et al. 2018

Journal of Biological Chemistry

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Fruit Juice Spoilage by Alicyclobacillus: Detection and Control Methods—A Comprehensive Review

Sourri¹,

Tassou²,

Nychas

et al. 2022

Foods

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Fruit juices have an important place in humans’ healthy diet. They are considered to be shelf stable products due to their low pH that prevents the growth of most bacteria. However thermo-acidophilic endospore forming bacteria of the genus Alicyclobacillus have the potential to cause spoilage of commercially pasteurized fruit juices. The flat sour type spoilage, with absence of gas production but presence of chemical taint compounds (mostly guaiacol) and the ability of Alicyclobacillus spores to survive after pasteurization and germinate under favorable conditions make them a major concern for the fruit juice industry worldwide. Their special characteristics and presence in the fruit juice industry has resulted in the development of many isolation and identification methods based on cell detection (plating methods, ELISA, flow cytometry), nucleic acid analysis (PCR, RAPD-PCR, ERIC-PCR, DGGE-PCR, RT-PCR, RFLP-PCR, IMS-PCR, qPCR, and 16S rRNA sequencing) and measurement of their metabolites (HPLC, GC, GC-MS, GC-O, GC-SPME, Electronic nose, and FTIR). Early detection is a big challenge that can reduce economic loss in the industry while the development of control methods targeting the inactivation of Alicyclobacillus is of paramount importance as well. This review includes a discussion of the various chemical (oxidants, natural compounds of microbial, animal and plant origin), physical (thermal pasteurization), and non-thermal (High Hydrostatic Pressure, High Pressure Homogenization, ultrasound, microwaves, UV-C light, irradiation, ohmic heating and Pulse Electric Field) treatments to control Alicyclobacillus growth in order to ensure the quality and the extended shelf life of fruit juices.

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Alicyclobacillus spp.: New Insights on Ecology and Preserving Food Quality through New Approaches

et al. 2015

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Alicyclobacillus spp. includes spore-forming and thermo-acidophilic microorganisms, usually recovered from soil, acidic drinks, orchards and equipment from juice producers. The description of the genus is generally based on the presence of ω-fatty acids in the membrane, although some newly described species do not possess them. The genus includes different species and sub-species, but A. acidoterrestris is generally regarded as the most important spoiler for acidic drinks and juices. The main goal of this review is a focus on the ecology of the genus, mainly on the species A. acidoterrestris, with a special emphasis on the different phenotypic properties and genetic traits, along with the correlation among them and with the primary source of isolation. Finally, the last section of the review reports on some alternative approaches to heat treatments (natural compounds and other chemical treatments) to control and/or reduce the contamination of food by Alicyclobacillus.

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Discrimination of Alicyclobacillus Strains by Lipase and Esterase Fingerprints

Cited by 3 publications

References 18 publications

Fluorogenic structure activity library pinpoints molecular variations in substrate specificity of structurally homologous esterases

Fluorogenic structure activity library pinpoints molecular variations in substrate specificity of structurally homologous esterases

Fruit Juice Spoilage by Alicyclobacillus: Detection and Control Methods—A Comprehensive Review

Alicyclobacillus spp.: New Insights on Ecology and Preserving Food Quality through New Approaches

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