2004
DOI: 10.1016/j.meegid.2004.02.001
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Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP

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Cited by 475 publications
(315 citation statements)
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“…in frozen preserved samples. A fragment of the GDH gene of Giardia (432 bp) was amplified by seminested PCR using the primers GDHeF, GDHiR and GDHiF as previously described (Read et al 2004). Cryptosporidium species and genotypes were identified using a nested PCRbased protocol.…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…in frozen preserved samples. A fragment of the GDH gene of Giardia (432 bp) was amplified by seminested PCR using the primers GDHeF, GDHiR and GDHiF as previously described (Read et al 2004). Cryptosporidium species and genotypes were identified using a nested PCRbased protocol.…”
mentioning
confidence: 99%
“…A fragment of the 60 kDa glycoprotein (GP60) gene was amplified by nested-PCR (Peng et al 2003) using primers 5'-ATAGTCTC-CGCTGTATTC-3' and 5'-GCAGAGGAACCAGCATC-3' (primary PCR) and 5'-TCCGCTGTATTCTCAGCC-3' and 5'-GAGATATATCTTGGTGCG-3' (secondary PCR). The G. duodenalis genotypes were determined by restriction fragment length polymorphism (RFLP) analysis as previously described (Read et al 2004). PCR-RFLP analysis of the secondary PCR products for the SSU-rRNA gene was performed using SspI, VspI, DdeI and MboII to differentiate Cryptosporidium species and genotypes (Xiao et al 2001;Feng et al 2007).…”
mentioning
confidence: 99%
“…in hemolymph, DFA-positive CSL feces, and DFA-positive mussel homogenate was based on two loci used for molecular characterization: the glutamate dehydrogenase (GDH) gene (31) and the beta-giardin gene (32). A 432-bp fragment was amplified using a modified GDH PCR protocol (31).…”
Section: Methodsmentioning
confidence: 99%
“…in hemolymph, DFA-positive CSL feces, and DFA-positive mussel homogenate was based on two loci used for molecular characterization: the glutamate dehydrogenase (GDH) gene (31) and the beta-giardin gene (32). A 432-bp fragment was amplified using a modified GDH PCR protocol (31). The PCR mixtures contained 5 l (1ϫ) 10ϫ PCR buffer with 1.5 mM MgCl 2 , 1 l (0.2 mM) dNTPs, 0.2 l (0.4 g/ml reaction) 10% bovine serum albumin (BSA), 1.25 l (25 pmol/reaction) of each primer at a concentration of 20 pmol/ l, 0.25 l (1.25 U) HotStarTaq Plus polymerase and 3 l and 1 l DNA for round 1 and round 2, respectively, and PCR water in a 50-l total volume.…”
Section: Methodsmentioning
confidence: 99%
“…Recently, some investigators have proposed species-specific names for the different genotypes of G. duodenalis [ Table-1] [40,41]. Genotypes A and B of G. duodenalis have been the only genotypes involved in human infection [2,8,10,42].…”
Section: Molecular Epidemiology Of Giardiamentioning
confidence: 99%