Discrimination of Bacterial Community Structures among Healthy, Gingivitis, and Periodontitis Statuses through Integrated Metatranscriptomic and Network Analyses

Nemoto, Takashi; Shiba, Takahiko; Komatsu, Keiji; Watanabe, Takayasu; Shimogishi, Masahiro; Shibasaki, Masaki; Koyanagi, Tatsuro; Nagai, Takahiko; Katagiri, Seiji; Takeuchi, Yasuo; Iwata, Takanori

doi:10.1128/msystems.00886-21

Cited by 28 publications

(28 citation statements)

References 58 publications

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“…Taxon pairs with SparCC values ≥0.9 are considered as having a co-occurrence relationship with a positive correlation. The parameters for this analysis other than the SparCC value were as previously described (Shiba et al, 2016(Shiba et al, , 2021Komatsu et al, 2020;Nemoto et al, 2021). The networks were visualized using the Cytoscape software v.2.8.…”

Section: Discussionmentioning

confidence: 99%

High‐frequency pulsed diode laser irradiation inhibits bone resorption in mice with ligature‐induced periodontitis

Ohsugi

Hatasa

Katagiri

et al. 2022

J Clinic Periodontology

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Aim The purpose of this study was to elucidate the suppressive effect of high‐frequency pulsed diode laser irradiation on bone resorption and its biological effects on gene expression and microbiome composition on the gingival tissue in ligature‐induced periodontitis in mice. Materials and Methods Ligating ligature around the teeth and/or laser irradiation was performed on the gingival tissue in mice as follows: Co (no ligature and no laser irradiation), Li (ligation without laser irradiation), La (no ligature but with laser irradiation), and LiLa (ligation with laser irradiation). Bone resorption was evaluated using micro‐computed tomography. RNA‐seq analysis was performed on gingival tissues of all four groups at 3 days after ligation. The differences in microbial composition between Li and LiLa were evaluated based on the number of 16S rRNA gene sequences. Results Bone resorption caused by ligation was significantly suppressed by laser irradiation. RNA‐seq in Co and La gingival tissue revealed many differentially expressed genes, suggesting diode laser irradiation altered gene expression. Gene set enrichment analysis revealed mTORC1 signalling and E2F target gene sets were enriched in gingival tissues both in La and LiLa compared with that in Co and Li, respectively. The amount of extracted DNA from ligatures was reduced by laser irradiation, and bacterial network structure was altered between the Li and LiLa. Conclusions High‐frequency pulsed diode laser irradiation showed biological effects and suppressed bone resorption in ligature‐induced periodontitis.

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Section: Discussionmentioning

confidence: 99%

High‐frequency pulsed diode laser irradiation inhibits bone resorption in mice with ligature‐induced periodontitis

Ohsugi

Hatasa

Katagiri

et al. 2022

J Clinic Periodontology

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“…Subgingival plaque samples collected from periodontitis patients and periodontitis-free individuals differ from each other [ 18 ]. P. gingivalis, T. forsythia , and T. denticola , the so-called red complex, have shown the strongest association with periodontal disease [ 19 ].…”

Section: Periodontitis: Lessons Learned From Established and Emerging...mentioning

confidence: 99%

Aggregatibacter actinomycetemcomitans and Filifactor alocis: Two exotoxin-producing oral pathogens

Ozuna

Snider

Belibasakis

et al. 2022

Front. Oral. Health

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Periodontitis is a dysbiotic disease caused by the interplay between the microbial ecosystem present in the disease with the dysregulated host immune response. The disease-associated microbial community is formed by the presence of established oral pathogens like Aggregatibacter actinomycetemcomitans as well as by newly dominant species like Filifactor alocis. These two oral pathogens prevail and grow within the periodontal pocket which highlights their ability to evade the host immune response. This review focuses on the virulence factors and potential pathogenicity of both oral pathogens in periodontitis, accentuating the recent description of F. alocis virulence factors, including the presence of an exotoxin, and comparing them with the defined factors associated with A. actinomycetemcomitans. In the disease setting, possible synergistic and/or mutualistic interactions among both oral pathogens might contribute to disease progression.

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“…Later, Nowicki et al (2018) analyzed the transition of dental conditions from healthy to having gingivitis and identified genes associated with early disease. Nemoto et al (2021) described a health-toperiodontitis microbiome shift and identified core taxa associated with periodontal disease progression, and Huang et al (2021) characterized temporal dynamics of the microbiome in gingivitis-to-periodontitis transition. Yu et al (2019) reported that the composition of the submucosal and subgingival microbiota at periodontitis and peri-implantitis sites was similar in subjects in the same cohort.…”

Section: Introductionmentioning

confidence: 99%

Correlations among core species corresponding to the clinical staging of periodontitis

ZHANG¹,

Zhen²,

LI³

et al. 2023

Biocell

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The correlation between microbiota plays a vital role in the progression of periodontal disease. This study investigated the in situ interaction networks between periodontal pathogens in periodontal and peri-implant disease.We used quantitative real-time polymerase chain reaction and Pearson's correlation coefficients to quantify the copy numbers and correlations of four oral core species-Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, and Streptococcus gordonii-from 80 subgingival sites (healthy and with periodontitis or gingivitis) in patients with periodontitis, and 68 subgingival sites (healthy and with periodontitis, gingivitis, peri-implantitis, or peri-implant mucositis) in patients with implants. The highest bacterial counts were observed for Porphyromonas gingivalis and Prevotella intermedia at all the sites. Within the same cohorts, the bacterial loads were greater at diseased sites than at healthy sites. Bacterial counts did not differ among clinical sites in the same group (P > 0.05) but differed between periodontitis and peri-implant mucositis sites in the two groups. Porphyromonas gingivalis, F. nucleatum, and Prevotella intermedia had strong correlations at gingivitis and healthy sites and moderate correlations at periodontitis sites in patients with periodontitis. In patients with implants, Prevotella intermedia, F. nucleatum, and S. gordonii had strong correlations only at peri-implantitis sites. Also, based on metagenomic analysis, F. nucleatum and Prevotella intermedia were significantly correlated at the subgingival plaque in peri-implantitis and periodontitis samples. Our results suggest that variations in microbe-microbe interactions in subgingival plaque reflect changes in the progression of periodontal disease, providing a new perspective for understanding the mechanisms of periodontitis and peri-implantitis.

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Discrimination of Bacterial Community Structures among Healthy, Gingivitis, and Periodontitis Statuses through Integrated Metatranscriptomic and Network Analyses

Abstract: The characteristics of the periodontal microbiome influence clinical periodontal status. Gingivitis involves reversible gingival inflammation without alveolar bone resorption.

Cited by 28 publications

References 58 publications

High‐frequency pulsed diode laser irradiation inhibits bone resorption in mice with ligature‐induced periodontitis

High‐frequency pulsed diode laser irradiation inhibits bone resorption in mice with ligature‐induced periodontitis

Aggregatibacter actinomycetemcomitans and Filifactor alocis: Two exotoxin-producing oral pathogens

Correlations among core species corresponding to the clinical staging of periodontitis

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