Discrimination of Banana Genotypes for Fusarium Wilt Resistance in the Greenhouse

Ribeiro, L. R.; Amorim, Édson Perito; Cordeiro, Z. J. M.; Silva, S. de Oliveira e; Dita, Miguel

doi:10.17660/actahortic.2011.897.52

Cited by 21 publications

(18 citation statements)

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“…1). In contrast to other reported bioassays (Smith et al, 2008;Mohamed et al, 2000;Ribeiro et al, 2011), the incubation period of TR4 was short (7 dai), even in older plants. This suggests an augmented virulence of Foc TR4 on 'Grand Naine' compared to compatible interactions involving Foc race 1.…”

Section: Resultscontrasting

confidence: 68%

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A Greenhouse Bioassay for the Fusarium Oxysporum F. Sp. Cubense X 'Grand Naine' (Musa, Aaa, Cavendish Subgroup) Interaction

Rodriguez

Waalwijk²,

Paiva³

et al. 2011

Acta Hortic.

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Several disease resistance screening protocols for Fusarium wilt of banana (causal agent Fusarium oxysporum f. sp. cubense -Foc) under greenhouse conditions have been reported. Here, we report a standardised rapid and reliable greenhouse bioassay for this pathosystem. This is indispensable for banana phenotyping, particularly since the occurrence of tropical race 4 (TR4), which is a significant threat for the global Cavendish-based banana export industry. Using a double-pot system, hardened 3-months-old tissue-culture plants of 'Grand Naine' (AAA, Cavendish subgroup) were individually inoculated with three TR4 isolates and one race 1 isolate with known pathogenicity on 'Silk' (AAB) bananas. All TR4 isolates caused similar symptoms and no differences regarding incubation period or severity were observed. In addition, all TR4 isolates were successfully recovered from the symptomatic rhizomes on Komada medium.

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Section: Resultscontrasting

confidence: 68%

“…These results confirm the compatible and incompatible interactions of 'Grand Naine' with Foc TR4 and race 1, respectively. The latter strain was compatible on 'Silk' plants (Ribeiro et al, 2011).…”

Section: Resultsmentioning

confidence: 91%

A Greenhouse Bioassay for the Fusarium Oxysporum F. Sp. Cubense X 'Grand Naine' (Musa, Aaa, Cavendish Subgroup) Interaction

Rodriguez

Waalwijk²,

Paiva³

et al. 2011

Acta Hortic.

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“…Considering the experimental design and the reaction of the susceptible ‘Brazilian’, this might be largely related to lower inoculum concentration in the field rather than specific edaphic conditions. Furthermore, this is an indication that, unlike M. basjoo and M. itinerans , which were not affected by Foc‐TR4 under any conditions, the resistance in these genotypes could be quantitative and eventually overcome by higher inoculum pressure as previously reported for other genotypes (Pegg & Langdon, ; Smith & Hamill, ; Ribeiro et al ., ). Unfortunately, the quantity of the inoculum of Foc‐TR4 in the field could not be estimated.…”

Section: Discussionmentioning

confidence: 97%

“…However, as reported by Ribeiro et al . (), field studies can also result in disease escape due to irregular inoculum distribution or specific local edaphic conditions. In the current study, the field trial was conducted in an area heavily infested with Foc‐TR4, and PCR‐based detection analysis confirmed the pathogen's presence in all the planting sites of the experiment.…”

Section: Discussionmentioning

confidence: 99%

“…For a crop such as banana, where breeding represents a long and time-consuming process, the phenotyping process to identify and select resistance sources needs to be as reliable as possible. Several protocols to infect banana with Foc in vitro (Wu et al, 2010) or under greenhouse conditions (Sun & Su, 1984;Smith et al, 2008;Dita et al, 2011;Ribeiro et al, 2011) have been reported. The current study used a twostep process comprising a combination of greenhouse and field studies that provided comprehensive and reliable information regarding disease reaction on the evaluated genotypes (Table 2; Figs S2 & S3).…”

Section: Discussionmentioning

confidence: 99%

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Resistance sources to Fusarium oxysporum f. sp. cubense tropical race 4 in banana wild relatives

Dita

et al. 2015

Plant Pathology

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Target trait evaluation in crop wild relatives is an important prerequisite for efficiently using the potential useful genes located in this valuable germplasm. Over recent decades, Fusarium oxysporum f. sp. cubense tropical race 4 (Foc‐TR4) has seriously threatened worldwide banana plantations. Breeding new resistant cultivars from wild banana species is expected to provide invaluable additional resources. However, knowledge on resistance to Foc‐TR4 in wild Musa species is very limited. In this study, eight genotypes of wild banana relatives (Musa acuminata subsp. burmannica, M. balbisiana, M. basjoo, M. itinerans, M. nagensium, M. ruiliensis, M. velutina and M. yunnanensis) were characterized for resistance to Foc‐TR4 in both greenhouse and field conditions. Most wild bananas showed higher resistance levels to Foc‐TR4 than the reference cultivars ‘Brazilian’ (AAA, susceptible) and ‘Goldfinger’ (AAAB, moderate resistance). Among the wild species, M. balbisiana showed the highest levels of disease intensity followed by M. acuminata subsp. burmannica. Some individuals of M. yunnanensis, M. nagensium, M. ruiliensis and M. velutina showed low levels of rhizome discolouration in greenhouse conditions, but were resistant in the field. No symptoms were observed on M. basjoo and M. itinerans, suggesting higher levels of resistance to Foc‐TR4. The results revealed different sources of resistance to Foc‐TR4 in banana wild relatives, which constitute a valuable genetic resource for banana breeding programmes aiming to produce cultivars resistant to fusarium wilt.

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Pre-Screening of Banana Genotypes for Fusarium Wilt Resistance by Using an In Vitro Bioassay

2022

Efficient Screening Techniques to Identify Mutants With TR4 Resistance in Banana

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In the process of breeding and selection of banana for resistance to Fusarium wilt, it is important to conduct an efficient resistance screening test by artificial inoculation with Fusarium oxysporum f. sp. cubense (Foc) Tropical Race 4. So far, there are two types of early bioassays for screening Musa genotypes against Foc: a greenhouse and an in vitro bioassay. The most commonly used greenhouse bioassay is a pot-based system followed by a hydroponic system. Here we describe an in vitro bioassay characterized by in vitro inoculation of rooted banana plantlets grown on medium consisting of half-strength MS macronutrients and MS micronutrients. The disease response and evaluation results obtained through this in vitro bioassay correlates with that from a greenhouse screen and/or field evaluation. Given the importance of in vitro cell and tissue culture techniques for banana (mutation) breeding, promising resistant clones could be screened directly. This in vitro bioassay is a totally contained system compared with greenhouse methods and does not require an acclimatization step, thereby improving banana breeding efficiency. The in vitro pre-screening protocol and bioassay for Fusarium wilt resistance presented here is fast, space-effective, and accurate.

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Discrimination of Banana Genotypes for Fusarium Wilt Resistance in the Greenhouse

Cited by 21 publications

References 0 publications

A Greenhouse Bioassay for the Fusarium Oxysporum F. Sp. Cubense X 'Grand Naine' (Musa, Aaa, Cavendish Subgroup) Interaction

A Greenhouse Bioassay for the Fusarium Oxysporum F. Sp. Cubense X 'Grand Naine' (Musa, Aaa, Cavendish Subgroup) Interaction

Resistance sources to Fusarium oxysporum f. sp. cubense tropical race 4 in banana wild relatives

Pre-Screening of Banana Genotypes for Fusarium Wilt Resistance by Using an In Vitro Bioassay

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