Discrimination of C3H4+ isomeric ions by charge inversion mass spectrometry using an alkali metal target

Hayakawa, Shigeo; Endoh, H.; Arakawa, Kazuo; Morishita, Norio; Sugiura, Toshio

doi:10.1016/0168-1176(95)04309-8

Cited by 23 publications

(11 citation statements)

References 17 publications

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“…Charge‐inversion mass spectrometry was performed using an MS/MS instrument in which mass‐separated positive ions were made to collide with one of the alkali‐metal targets of Na, K, and Cs, and the resulting negative ions formed upon two successive single electron transfers were mass‐analyzed 5, 13, 14. The MS/MS instrument utilizes a Hitachi M80B double‐focusing mass spectrometer as MS‐I to mass‐separate precursor ions and a toroidal electrostatic analyzer (ESA) as MS‐II to mass‐analyze secondary ions.…”

Section: Methodsmentioning

confidence: 99%

Differences between collisionally activated and electron‐transfer dissociations found for CH₂X₂(X = Cl, Br, and I) by using alkali‐metal targets

2008

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High-energy collisionally activated dissociation (HE-CAD) and high-energy electron- transfer dissociation (HE-ETD) on collisions with alkali-metal targets (Cs, K, and Na) were investigated for CH(2)X(2) (+) (X = Cl, Br, and I) ions by tandem mass spectrometry (MS/MS). In the HE-CAD spectra observed, peaks associated with CH(2)X(+) ions formed by a loss of a halogen atom are always predominant regardless of precursor ions and target metals. The observation of the predominant CH(2)X(+) ions is explained by the lowest energy levels of the fragments of CH(2)X(+) + X among the possible fragment energy levels and internal-energy distribution in HE-CAD. In the charge-inversion spectra, relative peak intensities of the negative ions formed by HE-ETD strongly depend on the precursor ions and the target metals. While the CHCl(2) (-) ion was predominant in the spectra of CH(2)Cl(2) (+) regardless of target species, the most intense peaks in those of CH(2)Br(2) (+) and CH(2)I(2) (+) were ascribed to either Br(-) or CH(2)Br(-) and either I(-) or I(2) (-), respectively, depending on the target metals. The dependence of the relative intensities of the fragment ions by HE-ETD on the precursor ions and target species are discussed on the basis of the energy levels of the neutral fragments and the narrow internal-energy distribution resulting from the near-resonant neutralization. It was demonstrated that HE-ETD using the alkali-metal targets provided rich information on the dissociation of the neutral species.

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Section: Methodsmentioning

confidence: 99%

Differences between collisionally activated and electron‐transfer dissociations found for CH₂X₂(X = Cl, Br, and I) by using alkali‐metal targets

2008

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“…Most charge inversion reactions have been performed in the keV energy range mainly because at these energies electron stripping and electron transfer reactions have effective cross sections. Charge inversion mass spectrometry using alkali metal gaseous targets has provided information about the dissociation pathways of energy‐selected neutral species 24, 26–32. Furthermore, differences in the chemical nature of positive and negative ions have allowed charge inversion mass spectrometry to be successfully applied to the problem of isomeric differentiation in the gas phase.…”

Section: Introductionmentioning

confidence: 99%

Charge inversion mass spectrometry: dissociation of resonantly neutralized molecules

Hayakawa

2004

J. Mass Spectrom.

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Charge inversion mass spectrometry is an MS/MS method in which the electric charge of the precursor ions is opposite to that of the secondary product ions. Charge inversion mass spectrometry is classified into four types depending on the electric charge and time scale of collisions. Charge inversion mass spectrometry using collisions with gaseous targets in the keV energy collision range has provided insights into the structures and reactions of ions and neutral molecules. The characteristics of charge inversion experiments are presented in terms of the reaction endothermicities and the cross sections and their dependence on the target species. In the case of rare-gas or simple molecular targets, double-electron transfer in one collision is effective to form positive ions from negative ions, while, in the case of alkali metal targets, successive single-electron transfers in two collisions is effective to form negative ions from positive ions. On the basis of the observed target-density dependence of the product ion intensity and thermochemical considerations for internal energy distribution using thermometer molecules, the charge inversion processes using alkali metal targets have been confirmed to occur by electron transfers in successive collisions and the dissociation processes are found to occur in energy-selected neutral species formed from near-resonant neutralization with alkali metal targets. While collisionally activated dissociation (CAD) is due to dissociation of activated ions with broad internal energy distributions, the charge inversion process using alkali metal targets is due to dissociation of energy-selected neutral species with narrow internal energy distributions. The charge inversion/alkali metal spectra provide clear differentiation of the isomeric cations of C(2)H(2), C(3)H(4) and dichlorobenzenes. The CAD spectra of these isomeric cations are similar.

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“…Charge inversion mass spectrometry using alkali metal targets [23][24][25][26][27][28][29] for singly protonated peptides and amino acids provided the information of the radical traps in the dissociation mechanism of the charge reduced peptides [28,30,31]. N-C␣ bond cleavages induced by electron-transfer processes of multiply charged peptides upon collision with alkali metals have been reported by Hvelplund and coworkers [32][33][34][35][36][37].…”

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confidence: 99%

“…The charge reduced peptide cation radicals formed by ECD and ETD are known to dissociate at various positions via the low-energy transitionstate for bond cleavage [16 -20]. Differences between ECD and ETD are also reported [15,21,22] .Charge inversion mass spectrometry using alkali metal targets [23][24][25][26][27][28][29] for singly protonated peptides and amino acids provided the information of the radical traps in the dissociation mechanism of the charge …”

mentioning

confidence: 99%

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High-energy electron transfer dissociation (HE-ETD) using alkali metal targets for sequence analysis of post-translational peptides

Hayakawa

Matsumoto

Hashimoto

et al. 2010

J. Am. Soc. Mass Spectrom.

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Post-translational modifications (PTMs) of proteins are important in the activation, localization, and regulation of protein function in vivo. The usefulness of electron capture dissociation (ECD) and electron-transfer dissociation (ETD) in tandem mass spectrometry (MS/MS) using low-energy (LE) trap type mass spectrometer is associated with no loss of a labile PTM group regarding peptide and protein sequencing. The experimental results of high-energy (HE) collision induced dissociation (CID) using the Xe and Cs targets and LE-ETD were compared for doubly-phosphorylated peptides TGFLT(p)EY(p)VATR (1). Although HE-CID using the Xe target did not provide information on the amino acid sequence, HE-CID using the Cs target provided all the z-type ions without loss of the phosphate groups as a result of HE-ETD process, while LE-ETD using fluoranthene anion gave only z-type ions from z 5 to z 11 . The difference in the results of HE-CID between the Xe and Cs targets demonstrated that HE-ETD process with the Cs target took place much more dominantly than collisional activation. The difference between HE-ETD using Cs targets and LE-ETD using the anion demonstrated that mass discrimination was much weaker in the high-energy process. HE-ETD was also applied to three other phosphopeptides YGGMHRQEX(p)VDC (2: X ϭ S, 3: X ϭ T, 4: X ϭ Y). The HE-CID spectra of the doubly-protonated phosphopeptides () of 2, 3, and 4 using the Cs target showed a very similar feature that the c-type ions from c 7 to c 11 and the z-type ions from z 7 to z 11 were formed via N-C␣ bond cleavage without a loss of the phosphate group. (J Am Soc Mass Spectrom 2010, 21, 1482-1489) © 2010 American Society for Mass Spectrometry P ost-translational modifications (PTMs) of proteins are important in vivo, especially, reversible protein phosphorylation, principally localized on serine, threonine, or tyrosine residues, is one of the most important and well-studied. Phosphorylations play crucial roles in the regulation of cellular processes such as cell cycle, cell growth, apoptosis, and signal transduction [1,2]. Although the mass analysis of phosphorylated proteins and peptides has become possible since the development of electrospray ionization (ESI) technique [3], the determination of phosphorylated positions has not yet been achieved by the most popular dissociation method, namely, a low-energy collisionally activated dissociation (LE-CAD) performed by using the tandem mass spectrometry (MS/ MS) [4]. Electron capture dissociation (ECD) using a Fourier transform ion cyclotron resonant mass spectrometer (FT-ICR-MS) [5][6][7][8] and electron-transfer dissociation (ETD) using a linear trap mass spectrometer [9,10] can provide information on c-and z-type ions [11,12] produced via N-C␣ backbone cleavage with no loss of labile PTM groups, such as phosphate and sulfate groups [13,14]. The N-C␣ backbone cleavage is different from other fragmentations brought about by collisional activation or infrared photoexcitation of closedshell cations [15]. The charge reduced p...

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Discrimination of C3H4+ isomeric ions by charge inversion mass spectrometry using an alkali metal target

Cited by 23 publications

References 17 publications

Differences between collisionally activated and electron‐transfer dissociations found for CH₂X₂(X = Cl, Br, and I) by using alkali‐metal targets

Differences between collisionally activated and electron‐transfer dissociations found for CH₂X₂(X = Cl, Br, and I) by using alkali‐metal targets

Charge inversion mass spectrometry: dissociation of resonantly neutralized molecules

High-energy electron transfer dissociation (HE-ETD) using alkali metal targets for sequence analysis of post-translational peptides

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Discrimination of C3H4+ isomeric ions by charge inversion mass spectrometry using an alkali metal target

Cited by 23 publications

References 17 publications

Differences between collisionally activated and electron‐transfer dissociations found for CH2X2(X = Cl, Br, and I) by using alkali‐metal targets

Differences between collisionally activated and electron‐transfer dissociations found for CH2X2(X = Cl, Br, and I) by using alkali‐metal targets

Charge inversion mass spectrometry: dissociation of resonantly neutralized molecules

High-energy electron transfer dissociation (HE-ETD) using alkali metal targets for sequence analysis of post-translational peptides

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Product

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Differences between collisionally activated and electron‐transfer dissociations found for CH₂X₂(X = Cl, Br, and I) by using alkali‐metal targets

Differences between collisionally activated and electron‐transfer dissociations found for CH₂X₂(X = Cl, Br, and I) by using alkali‐metal targets