Discrimination of damaged/dead cells by propidium iodide uptake in immunofluorescently labeled populations analyzed by phase-sensitive flow cytometry

Steinkamp, John A.; Lehnert, Bruce E.; Lehnert, Nancy M.

doi:10.1016/s0022-1759(99)00053-8

Cited by 34 publications

(28 citation statements)

References 31 publications

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“…Assay of trapped K562 cell viability was performed using PI staining assay [39,40]. Briefly, after rising with PBS thrice, 1.0 mmol/L PI was introduced into the cell trapping chambers.…”

Section: On-chip Ctc Sorting and Viability Assaymentioning

confidence: 99%

“…The K562 cells cultured in the microdevice were treated with various concentrations (0.25, 0.5, and 1.0 g/L) of cytarabine for 6 h. Cell viability was optically observed and evaluated using PI staining assay following the methods reported previously [39,40]. After the treatment with cytarabine, the dead cells were observed to be wizened, anomalistic on the cell silhouette, gray in the micrograph, and stained as red dots in the fluorescent image (Supporting Information Fig.…”

Section: Assay Of Ctc Responses To An Anticancer Drugmentioning

confidence: 99%

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Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Liu

Wang

et al. 2010

Electrophoresis

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Metastasis caused by the entry of circulating tumor cells (CTCs) into the bloodstream or lymphatic vessels is a major factor contributing to death in cancer patients. Separation of CTCs and studies on CTC-drug interactions are very important for prognostic and therapeutic implications of metastatic cancer. In this study, an integrated microfluidic device for CTC separation through the combination of lectin and microstructure is presented. This microfluidic device and lectin concanavalin A were utilized for the separation of K562 cells in whole blood samples. The results showed that the separation efficiency can reach 84%, which is much higher than that of an experiment without concanavalin A treatment. To further demonstrate the feasibility of this microfluidic device application in sequential studies after target cells were separated, the interactions of K562 cells and an anticancer drug, cytarabine, were also examined. After 6 h on-chip treatment with cytarabine, the viabilities of K562 cells were 85.29, 77.05, and 40% for drug concentration levels of 0.25, 0.5, and 1.0 g/L, respectively. This system can facilitate the rapid and efficient in vitro investigation of CTC separation and CTC-related studies.

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“…Assay of trapped K562 cell viability was performed using PI staining assay [39,40]. Briefly, after rising with PBS thrice, 1.0 mmol/L PI was introduced into the cell trapping chambers.…”

Section: On-chip Ctc Sorting and Viability Assaymentioning

confidence: 99%

Section: Assay Of Ctc Responses To An Anticancer Drugmentioning

confidence: 99%

Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Liu

Wang

et al. 2010

Electrophoresis

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“…Este último valor, según Rohan (1960) Estos valores no coinciden con Braudeau (1970), quien obtuvo 2.63% de cenizas en cotiledones de cacao seco no fermentados mientras que en el estudio el promedio fue de 3.49%. Por otra parte, Steinkamp et al (1999) y Guerrón (2009) afirman que el cacao CCN-51 posee características predominantes de Trinitario con promedios de cenizas del 4.18% y en el cacao tipo Nacional de 3.62%; promedios que tienden a coincidir con los obtenidos en el presente estudio (Cuadro 4).…”

Section: Resultados Y Discusiónunclassified

Implementación De La PCR (Reacción en Cadena De La Polimerasa) Para El Diagnóstico De La Brucelosis De Bovinos en El Ecuador

Falquez¹,

Motte²,

Martínez³

et al. 2008

cyt

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Quevedo -El Empalme. Quevedo, Los Ríos, Ecuador cvallejo@uteq.edu.ec; oka.evel@hotmail.com; wmorales@uteq.edu.ec; rramos@uteq.edu.ec resumen L a investigación se realizó en la Finca Experimental "La Represa" propiedad de la Universidad Técnica Estatal de Quevedo y en el Laboratorio de Calidad de Cacao de la EET-Pichilingue-INIAP con una duración de cuatro meses (febrero-junio/2013). El objetivo fue caracterizar los atributos físico-químicos de almendras y sensoriales de pastas de quince clones de cacao; doce de tipo Nacional y tres testigos (CCN-51, EET-103 e IMC-67). Se utilizó un diseño completamente al azar con tres repeticiones. Las variables estudiadas fueron: índices de semilla y mazorca, número de almendras, peso de 100 semillas, porcentaje y pH de testa. Para la valoración química se registró: grasa, energía, y ceniza. Los atributos sensoriales (sabores, acidez, amargor, astringencia, dulce, cacao, floral, frutal, nuez y otros sabores se evaluaron usando una escala ordinal de 0 a 5; además, de los defectos crudo/verde y moho. Se realizó el análisis de Componentes Principales. El mayor índice de mazorca correspondió al DIRCYT-C129 (34.36 g); el DIRCYT-C103 tuvo mayor índice de semilla (1.65 g); en el peso de 100 semillas y contenido de grasa no hubo diferencias significativas (p>0.05); aunque CCN-51 fue ligeramente superior (41.44%). En atributos físicos fueron superiores los clones EET-103, CCN-51 y IMC-67. Todos los clones presentaron características químicas similares. El DIRCYT-C225 presentó el mejor perfil sensorial "sabor Arriba o floral", con alto potencial para la industria chocolatera.Palabras clave: almendra, teobromina, cacao, organoléptica y, calidad AbstrAct T his research was carried out at La Represa Experimental Farm, property of the State Technical University of Quevedo and at the Cocoa Quality Lab, belonging to EET-Pichilingue-INIAP for a period of four months (February-January 2013). The objective was to characterize the physical -chemical attributes of beans and sensory attributes of paste /pasta in fifteen clones of cocoa, being twelve of type Nacional and three witnesses (batons) (CCN-51, EET-103 e IMC-67). A completely randomized design with three repetitions was used. The studied variables were: seed and cob index, number of beans, 100 seed weight, head pH and percentage. For chemical evaluation fat, energy, and ash were registered. Sensory attributes (flavors, acidity, bitterness, astringency, and sweetness; cocoa, floral, fruits, nuts, and other flavors were evaluated using a 0 to 5 ordinal scale, besides raw/green and mold defects. Principal Components Analysis was made. The highest cob index was shown by DIRCYT-C129 (34.36 g); DIRCYT-C103 showed higher seed index (1.65 g.), concerning the 100 seed weight and fat content, no significant differences (p>0.05) were found, although CCN-51 was a little higher (41.44%). Concerning physical attributes, clones EET-103, CCN-51 y IMC-67 were better. All the clones showed similar chemical characteristics. DIRCYT-C225 presented the bes...

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“…Recently we employed differential subtraction combined with lifetime-based sensing to resolve nearly completely overlapping spectra from Hoechst 33342 (DNA content) and monobromobimane (glutathione) labeling (32). Phaseresolved fluorescence measurements also have been used to discriminate damaged/dead cells by popidium iodide uptake in murine thymus cells labeled with the tandem conjugate phycoerthrin/Texas Red having completely overlapping emission spectra with propidium iodide (33).…”

Section: Discussionmentioning

confidence: 99%

Enhanced immunofluorescence measurement resolution of surface antigens on highly autofluorescent, glutaraldehyde-fixed cells analyzed by phase-sensitive flow cytometry

et al. 1999

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Discrimination of damaged/dead cells by propidium iodide uptake in immunofluorescently labeled populations analyzed by phase-sensitive flow cytometry

Cited by 34 publications

References 31 publications

Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Implementación De La PCR (Reacción en Cadena De La Polimerasa) Para El Diagnóstico De La Brucelosis De Bovinos en El Ecuador

Enhanced immunofluorescence measurement resolution of surface antigens on highly autofluorescent, glutaraldehyde-fixed cells analyzed by phase-sensitive flow cytometry

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