Discrimination of Euglena gracilis strains Z and bacillaris by MALDI-TOF MS

Lukáčová, Alexandra; Beck, Terézia; Trnková, Katarína; Trniková, Miriam; Krajčovič, Juraj; Vesteg, Matej

doi:10.1111/jam.15600

Cited by 2 publications

(1 citation statement)

References 61 publications

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“…Matrix-assisted laser desorption-ionization (MALDI) is a soft ionization technique appropriate for the analysis of biomolecules such as nucleic acids, proteins, peptides and lipids [22]. It has become an attractive method for the rapid identification of bacteria [23][24][25], fungi [26][27][28], yeasts [29] and protists [30], even for subspecies-level typing [31]. Claydon et al first used MALDI-TOF MS to obtain whole-cell spectral fingerprints of Escherichia coli, Staphylococcus saprophyticus, Staphylococcus aureus, Staphylococcus epidermidis, Citrobacter freundii, Klebsiella aerogenes and Mycobacterium smegmatis in 1996 [32].…”

Section: Introductionmentioning

confidence: 99%

Differentiation and Identification of Endophytic Bacteria from Populus Based on Mass Fingerprints and Gene Sequences

Wang,

Wu,

Han

et al. 2023

IJMS

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Plant endophytic bacteria play important roles in plants’ growth and resistance to stress. It is important to characterize endophytic bacteria to be able to understand their benefits. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has become a powerful technique for bacterial identification due to its high throughput and simple procedures. In this study, the endophytic bacteria separated from Populus (the leaves, roots and stems of Populus tomentosa Carrière; stems of Populus nigra Linn. var. nigra; and stems of Populus canadensis Moench) were identified and classified based on MALDI-TOF MS data and 16S rRNA gene sequencing. The sampling and preparation of bacteria were optimized to obtain meaningful protein mass fingerprints. The composite correlation index (CCI) values of the inter-genera and inter-species protein mass fingerprints demonstrated sufficient differences between the strains. In the CCI value matrix for ten species in the same genus, all the CCI values were less than 0.5. Among the species, 95.6% of all the CCI values were less than 0.5. After data processing, the classification capacity of the protein mass fingerprints was verified using inter-specific and inter-generic PCoA. To compare different methods’ potential for differentiation and phylogenetic analysis, a dendrogram of the MS profiles and a phylogenetic tree based on the 16S rRNA gene sequences were constructed using 61 endophytic bacteria found in Populus. The clustering and grouping results show that the phylogenetic analysis based on MALDI-TOF MS is similar to that based on 16S rRNA gene sequencing. This study provides a valuable reference for differentiating and identifying endophytic bacteria according to their protein mass fingerprints.

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