Discrimination of ‘fiber-type’ and ‘drug-type’ Cannabis sativa L. by fluorescent duplex PCR

Sutipatanasomboon, Arpaporn; Panvisavas, Nathinee

doi:10.1016/j.fsigss.2011.10.008

Cited by 6 publications

(5 citation statements)

References 4 publications

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“…DNA-based profiling techniques used for cannabis identification are reviewed by others [16,106] and the DNA sequence of some cultivars is already publicly available [11,12]. Based on the polymorphisms of the enzyme responsible for Δ 9 -THCA synthesis [14], fluorescent duplex-PCR test [82] and single nucleotide polymorphism assay [83] were developed and successfully applied to discriminate between drug-type and fiber-type cannabis. Recently, a candidate gene involved in phytocannabinoid biosynthesis has been identified that clearly distinguishes these two chemotypes [14] (see later in Section 2.3).…”

Section: Identification Of Cannabis and Its Productsmentioning

confidence: 99%

“…Due to their different phytocannabinoid composition, the distinct species and/or strains (chemotypes) have different medical and psychotropic potential. Therefore, the genetic background of the different chemotypes [69,77,80,81] as well as their chemotaxonomy especially in terms of molecular/metabolic fingerprinting [6,7,10,42,82] have been extensively studied. Metabolic fingerprinting may be also used in forensic identification of the different chemotypes or during quality control and breeding/selection of the different drug-type cannabis lines [42,70].…”

Section: Introductionmentioning

confidence: 99%

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Cannabis: A Treasure Trove or Pandora's Box?

Solymosi

Köfalvi

2017

MRMC

122

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We summarize our current knowledge on the recreational use of cannabis with respect to the modes of consumption, short-term effects, chronic health consequences and cannabis use disorder. Next, we overview the molecular targets of a dozen major and minor bioactive cannabinoids in the body. This helps us introduce the endocannabinoid system in an unprecedented detail: its up-todate molecular biology, pharmacology, physiology and medical significance, and beyond. In conclusion, we offer an unbiased survey about cannabis to help better weigh its medical value versus the associated risks.

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Section: Identification Of Cannabis and Its Productsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cannabis: A Treasure Trove or Pandora's Box?

Solymosi

Köfalvi

2017

MRMC

122

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“…Following the definition of Sutipatanasomboon et al, with regard to the THC/CBD ratio, the cannabis plant can be divided into three categories, a drug type (ratio greater than 1) ( Figure 5), a fiber type (ratio less than 1) ( Figure 6) and an intermediate type (ratio close to 1) ( Figure 7) [17].…”

Section: Figure 4 Comparison Of Cbd Content Between Hplc-uv and Gc-fidmentioning

confidence: 99%

Differentiation between drug-type, fibre-type and intermediate-type in cannabis samples: HPLC-UV versus GC-FID

Kadkhodaei

Biei

Vidiella

et al. 2021

J Pharm Biopharm Res

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Cannabis sativa is known to be the most abused illegal drug worldwide. To date it is not only used as a medicine but has been established as a lifestyle product. The most relevant phytocannabinoids represent the ingredients delta-9-tetrahydrocannabinol (9-THC) and cannabidiol (CBD), whereby only 9-THC shows a psychoactive effect. Since 2017, the so-called CBD-hemp containing CBD as main ingredient is distributed in many countries as a legal alternative. In these products, 9-THC must not exceed a certain percentage. It is hardly possible to differentiate between THC-hemp and CBD-hemp presenting a major challenge for authorities. Therefore, there is the need to develop fast and efficient analysis methods to distinguish between fibre-type, drug-type and intermediate-type cannabis products. The aim of this study was to compare two simple and inexpensive HPLC-UV and GC-FID methods for their ability to quantify phytocannabinoids in dried cannabis plant material. For this purpose, a set of 37 fresh and dried cannabis samples randomly chosen from seizures of Austrian police was subject to complementary quantification of 9-THC and CBD. After having taken into account decomposition of certain phytocannabinoids, the result of this quantitative study showed good correlation between HPLC-UV and GC-FID regardless of quantifying cannabis leaves or buds.

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“…Although THC is the first thing that comes to mind when talking about cannabis, there are also nonpsychoactive cannabinoids with several therapeutic properties such as CBD, which has recently become more important due to THC limitations and new discoveries about CBD medicinal properties in parallel. Indeed, the plant’s adverse psychotropic and addictive effects are attributed to tetrahydrocannabinol [ 21 , 50 , 66 ]. The use of cannabis has always been very controversial because it is classified as an illegal drug due to its psychoactive THC.…”

Section: Phytocannabinoidsmentioning

confidence: 99%

It Is Our Turn to Get Cannabis High: Put Cannabinoids in Food and Health Baskets

et al. 2020

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Cannabis is an annual plant with a long history of use as food, feed, fiber, oil, medicine, and narcotics. Despite realizing its true value, it has not yet found its true place. Cannabis has had a long history with many ups and downs, and now it is our turn to promote it. Cannabis contains approximately 600 identified and many yet unidentified potentially useful compounds. Cannabinoids, phenolic compounds, terpenoids, and alkaloids are some of the secondary metabolites present in cannabis. However, among a plethora of unique chemical compounds found in this plant, the most important ones are phytocannabinoids (PCs). Over hundreds of 21-22-carbon compounds exclusively produce in cannabis glandular hairs through either polyketide and or deoxyxylulose phosphate/methylerythritol phosphate (DOXP/MEP) pathways. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are those that first come to mind while talking about cannabis. Nevertheless, despite the low concentration, cannabinol (CBN), cannabigerol (CBG), cannabichromene (CBC), tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), cannabinodiol (CBND), and cannabinidiol (CBDL) may have potentially some medical effects. PCs and endocannabinoids (ECs) mediate their effects mainly through CB1 and CB2 receptors. Despite all concerns regarding cannabis, nobody can ignore the use of cannabinoids as promising tonic, analgesic, antipyretic, antiemetic, anti-inflammatory, anti-epileptic, anticancer agents, which are effective for pain relief, depression, anxiety, sleep disorders, nausea and vomiting, multiple sclerosis, cardiovascular disorders, and appetite stimulation. The scientific community and public society have now increasingly accepted cannabis specifically hemp as much more than a recreational drug. There are growing demands for cannabinoids, mainly CBD, with many diverse therapeutic and nutritional properties in veterinary or human medicine. The main objective of this review article is to historically summarize findings concerning cannabinoids, mainly THC and CBD, towards putting these valuable compounds into food, feed and health baskets and current and future trends in the consumption of products derived from cannabis.

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Discrimination of ‘fiber-type’ and ‘drug-type’ Cannabis sativa L. by fluorescent duplex PCR

Cited by 6 publications

References 4 publications

Cannabis: A Treasure Trove or Pandora's Box?

Cannabis: A Treasure Trove or Pandora's Box?

Differentiation between drug-type, fibre-type and intermediate-type in cannabis samples: HPLC-UV versus GC-FID

It Is Our Turn to Get Cannabis High: Put Cannabinoids in Food and Health Baskets

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