Discrimination of Gonipterini weevil genera using near infrared spectroscopy

Johnson, Joel B.

doi:10.1177/09670335221117300

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…Возможности использования ИК-спектрометрии для таксономической идентификации установлены в различных областях [Rodríguez-Fernández et al, 2010;Vance et al, 2016], в том числе для бабочек [Tao et al, 2018], мух дрозофил , прямокрылых [Da Silva, Gutjahr, De Morais, 2018], комаров , долгоносиков [Johnson, 2022] и термитов [Jouquet et al, 2018]. Поэтому целью данного исследования являлось изучение химического состава тел дождевых червей разных родов и видов методом инфракрасной спектрометрии для установления возможности его использования в таксономической идентификации дождевых червей.…”

Section: Introductionunclassified

Investigation of differences in the IR spectra of earthworms as a possible way of their taxonomic identification

Kniazev,

Babiy,

Golovanova

et al. 2023

Вест. ПГУ. Биол.

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IR spectrometry is successfully used for the taxonomic identification of various groups of invertebrates: butterflies, flies, grasshoppers, mosquitoes, weevils, and termites. Therefore, the purpose of this study was to assess the fundamental possibility of taxonomic identification of earthworms based on the study of the functional-group composition of their bodies by the method of IR-Fourier spectroscopy. Seven species of earthworms from five genera were the objects of the study: Aporrectodea caliginosa, Eisenia fetida, Eisenia nordenskioldi, Lumbricus rubellus, Rhiphaeodrilus diplotetrathecus, Octolasion lacteum, Eisenia ventripapillata. IR absorption spectra were registered on an FT-801 IR-Fourier spectrometer in the range of 500–4000 cm–1. The position, intensity, and area of the absorption bands were determined on all spectra. The maximum differences between individual species in intensity and position of protein and nucleic acid absorption bands were established. Using the principal component method, it was shown that the classification was based on the characteristics of the absorption bands of stretching and bending vibrations of methyl and methylene groups, as well as amide groups in the structure of worms. At the same time, the division of earthworms into subgroups with similar spectral characteristics could be explained by several reasons: their origin, kinship, and the influence of the environment. The data obtained during the study confirm the possibility of using IR spectrometry for the identification of earthworms.

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Section: Introductionunclassified

Investigation of differences in the IR spectra of earthworms as a possible way of their taxonomic identification

Kniazev,

Babiy,

Golovanova

et al. 2023

Вест. ПГУ. Биол.

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Images in red: A methodological and integrative approach for the usage of Near-infrared Hyperspectral Imaging (NIR-HSI) on collection specimens of Orthoptera (Insecta)

Tavares,

da Silva,

Gutjahr

et al. 2024

Preprint

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Anthropogenic actions have caused severe environmental changes, leading to a rapid and massive loss of biodiversity, called the Holocene Extinction or "Sixth Extinction." In this context, integrative methods, capable of quickly and reliably determining and differentiating species, have become constantly demanded in taxonomic studies. Near-infrared spectroscopical technologies have proved to be promising for application in the integrative taxonomy of insects, and it is a true-non-destructive method, causing no damage to the sample and being completely chemical-preparation-free. Near-infrared spectral profiles are known as the fingerprints of the chemical composition of a given sample, and a new layer of information may be accessed. Hyperspectral imaging technologies in the near-infrared range are among the most popular, although their usage is still incipient in insect studies. As in other animal taxa, katydids' taxonomy has traditionally been based on morphological comparison, resulting in many misclassifications over the years. However, integrative methods are more and more required in taxonomic studies. Different methods and technologies have been used from an integrative perspective to minimize misidentifications, especially for non-taxonomist or untrained researchers. Here, we approach the applicability of near-infrared spectroscopy coupled with hyperspectral imaging technology to Ensifera specimens housed in collections, discussing the advantages, disadvantages, and challenges to future applications. As a result, we propose using only homologous body parts in comparing and modeling species using this kind of data due to the heterogeneity of the insects' exoskeleton. Additionally, we made a case study by discriminating four species of the katydid genus Conocephalus Thunberg, one of the most speciose genera within Orthoptera that is known to have polymorphic species, with variations expressed in the wing development and postabdomen appendages. We generated a Partial Least Squares-Discriminant Analysis (PLS-DA) classification model for the species with an overall classification accuracy for the assigned pixels of 90% and specific accuracy for pixel discrimination ranging from 96% to 98%. This is one of the few research studies employing hyperspectral imaging in insects' taxonomy, and it is the very first to use this technology in Orthoptera; therefore, this is a preliminary approach to usage as an integrative method.

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Discrimination of Gonipterini weevil genera using near infrared spectroscopy

Cited by 2 publications

References 37 publications

Investigation of differences in the IR spectra of earthworms as a possible way of their taxonomic identification

Investigation of differences in the IR spectra of earthworms as a possible way of their taxonomic identification

Images in red: A methodological and integrative approach for the usage of Near-infrared Hyperspectral Imaging (NIR-HSI) on collection specimens of Orthoptera (Insecta)

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