Discrimination of leaves of Panax ginseng and P. quinquefolius by ultra high performance liquid chromatography quadrupole/time-of-flight mass spectrometry based metabolomics approach

Mao, Qian; Bai, Min; Xu, Jin–Di; Kong, Ming; Zhu, Lin-Yin; Zhu, He; Wang, Qiang; Li, Songlin

doi:10.1016/j.jpba.2014.04.032

Cited by 74 publications

(37 citation statements)

References 28 publications

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“…These features make TOFMS very attractive for the analysis of complex herbal mixtures [122,176,181]. For example, Mao et al [182] used an UHPLC-qTOFMS-based approach to discriminate the leaves of American ginseng and Asian ginseng that cannot be easily discriminated based on their mere resemblance. As can be seen in Fig.…”

Section: Chromatographic and Hyphenated Methodsmentioning

confidence: 99%

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

Pferschy‐Wenzig

Bauer

2015

Epilepsy & Behavior

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Section: Chromatographic and Hyphenated Methodsmentioning

confidence: 99%

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

Pferschy‐Wenzig

Bauer

2015

Epilepsy & Behavior

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“…PC2 (negative scores) also showed the separation of inoculated PL-treated and non-inoculated groups (Fig 3). The separation of negative and positive groups in PC1 and PC2 is clearly linked to the differences of each metabolomic profile [39]. For this reason, OPLS-DA chemometric analysis was applied to the UPLC-QTOF-MS E data to compare samples according to metabolites that influenced on disease control against F. pallidoroseum in melons treated with PL.…”

Section: Resultsmentioning

confidence: 99%

Effect of pulsed light on postharvest disease control-related metabolomic variation in melon (Cucumis melo) artificially inoculated with Fusarium pallidoroseum

Filho

Silva

Lopes

et al. 2019

Preprint

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AbstractPulsed light, as a postharvest technology, is an alternative to traditional fungicides, and can be used on a wide variety of fruit and vegetables for sanitization or pathogen control. In addition to these applications, other effects also are detected in vegetal cells, including changes in metabolism and production of secondary metabolites, which directly affect disease control response mechanisms. This study aimed to evaluate the possible applications of pulsed ultraviolet light in controlling postharvest rot, mainly caused by Fusarium pallidoroseum in yellow melon ‘Goldex’, in natura, and its implications in the disease control as a function of metabolomic expression to effect fungicidal or fungistatic. The dose of pulsed light (PL) that inhibited F. pallidoroseum growth in melons (Cucumis melo var. Spanish) was 9 KJ m-2. Ultra performance liquid chromatography (UPLC) coupled to a quadrupole time-of-flight (QTOF) mass analyzer identified 12 compounds based on the MS/MS fragmentation patterns. Chemometric analysis by Principal Components Analysis (PCA) and Orthogonal Partial Least Squared Discriminant Analysis (OPLS-DA and S-plot) were used to evaluate the changes in fruit metabolism. PL technology provided protection against postharvest disease in melons, directly inhibiting the growth of F. pallidoroseum through upregulation of specific fruit biomarkers such as pipecolic acid (11), saponarin (7), and orientin (3), which acted as major markers for the defense system against pathogens. PL can thus be proposed as a postharvest technology to avoid chemical fungicides and may be applied to reduce the decay of melon quality during its export and storage.

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“…Recent IonKey/MS Ion Mobility technique developed by Waters Corporation provide the feasibility of differentiating the isomers [35]. Compounds Rk 1 , Rg 5 , Rh 2 , Gypenoside XVII, Compound Mc-1, Compound O, Gypenoside IX, Majonoside R 1 , notoginsenoside N 1 and notoginsenoside A were detected in white ginseng for the first time, and they were found in red ginseng [26] or the leaves of ginseng [27]. …”

Section: Resultsmentioning

confidence: 99%

Component analysis and target cell-based neuroactivity screening of Panax ginseng by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry

Jie

Chen

Liang

et al. 2016

Journal of Chromatography B

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Ginseng is one of the most widely used natural medicines in the world. Recent studies have suggested Panax ginseng has a wide range of beneficial effects on aging, central nervous system disorders, and neurodegenerative diseases. However, knowledge about the specific bioactive components of ginseng is still limited. This work aimed to screen for the bioactive components in Panax ginseng that act against neurodegenerative diseases, using the target cell-based bioactivity screening method. Firstly, component analysis of Panax ginseng extracts was performed by UPLC-QTOF-MS, and a total of 54 compounds in white ginseng were characterized and identified according to the retention behaviors, accurate MW, MS characteristics, parent nucleus, aglycones, side chains, and literature data. Then target cell-based bioactivity screening method was developed to predict the candidate compounds in ginseng with SH-SY5Y cells. Four ginsenosides, Rg2, Rh1, Ro, and Rd, were observed to be active. The target cell-based bioactivity screening method coupled with UPLC-QTOF-MS technique has suitable sensitivity and it can be used as a screening tool for low content bioactive constituents in natural products.

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Discrimination of leaves of Panax ginseng and P. quinquefolius by ultra high performance liquid chromatography quadrupole/time-of-flight mass spectrometry based metabolomics approach

Cited by 74 publications

References 28 publications

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

The relevance of pharmacognosy in pharmacological research on herbal medicinal products

Effect of pulsed light on postharvest disease control-related metabolomic variation in melon (Cucumis melo) artificially inoculated with Fusarium pallidoroseum

Component analysis and target cell-based neuroactivity screening of Panax ginseng by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry

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