Discrimination of menstrual and peripheral blood traces using attenuated total reflection Fourier transform-infrared (ATR FT-IR) spectroscopy and chemometrics for forensic purposes

Mistek-Morabito, Ewelina; Lednev, Igor K.

doi:10.1007/s00216-021-03206-w

Cited by 27 publications

(14 citation statements)

References 48 publications

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“…Of the methods discussed, the most promising were ATR-FTIR and Raman spectroscopies due to the specificity (approximately 100% accuracy) and nondestructive nature of the tests. Most recently, Mistek-Morabito et al paired ATR-FTIR spectroscopy with chemometrics to distinguish between menstrual and peripheral blood . A PLS-DA model was developed using 19 menstrual and 24 peripheral blood samples, for a total of 43 samples.…”

Section: Biologymentioning

confidence: 99%

“…Most recently, Mistek-Morabito et al paired ATR-FTIR spectroscopy with chemometrics to distinguish between menstrual and peripheral blood. 29 A PLS-DA model was developed using 19 menstrual and 24 peripheral blood samples, for a total of 43 samples. External validation was done to ensure the robust nature of the model, and it was performed with 100% accuracy.…”

Section: ■ Introductionmentioning

confidence: 99%

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Innovative Vibrational Spectroscopy Research for Forensic Application

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Section: Biologymentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Innovative Vibrational Spectroscopy Research for Forensic Application

et al. 2023

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“…The biochemical methods used in this type of analyses, such as immunochromatography and Real-Time PCR, are sophisticated and can only be carried out in a forensic laboratory [71,72]. To overcome these challenges, some researchers explored the potential use of ATR-FTIR in differentiating between stains of various types of blood [55,58,73,74]. The earliest study that examined the difference between ATR-FTIR spectra of stains of menstrual and venous bloods was carried out in 2017.…”

Section: Discrimination Between Different Types Of Human Bloodmentioning

confidence: 99%

Attenuated total reflection-Fourier transform infrared spectroscopy: a universal analytical technique with promising applications in forensic analyses

Alkhuder

2022

Int J Legal Med

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Contemporary criminal investigations are based on the statements made by the victim and the eyewitnesses. They also rely on the physical evidences found in the crime scene. These evidences, and more particularly biological ones, have a great judicial value in the courtroom. They are usually used to revoke the suspect's allegations and confirm or refute the statements made by the victim and the witnesses. Stains of body fluids are biological evidences highly sought by forensic investigators. In many criminal cases, the success of the investigation relies on the correct identification and classification of these stains. Therefore, the adoption of reliable and accurate forensic analytical methods seems to be of vital importance to attain this objective. Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) is a modern and universal analytical technique capable of fingerprint recognition of the analyte using minimal amount of the test sample. The current systematic review aims to through light on the fundamentals of this technique and to illustrate its wide range of applications in forensic investigations. ATR-FTIR is a nondestructive technique which has demonstrated an exceptional efficiency in detecting, identifying and discriminating between stains of various types of body fluids usually encountered in crime scenes. The ATR-FTIR spectral data generated from bloodstains can be used to deduce a wealth of information related to the donor species, age, gender, and race. These data can also be exploited to discriminate between stains of different types of bloods including menstrual and peripheral bloods. In addition, ATR-FTIR has a great utility in the postmortem investigations. More particularly, in estimating the postmortem interval and diagnosing death caused by extreme weather conditions. It is also useful in diagnosing some ambiguous death causes such as fatal anaphylactic shock and diabetic ketoacidosis.

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“…Attenuated total reflectance (ATF) FT-IR spectroscopy [81,82] has been used to characterise a number of body fluids, accommodating the age of the stain and excluding unexpected non-target components. Similar studies have also been undertaken on semen [83,84], with methods developed to identify and avoid interference from a number of environmental factors; on dried urine [85] where the gender of the donor was able to be deciphered; and to distinguish between menstrual and peripheral blood [86]. The alternative modification, external reflection FT-IR spectroscopy, has also been proposed [59] to specifically identify body fluids associated with sexual assault.…”

Section: Spectroscopic Techniquesmentioning

confidence: 99%

On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime

Sijen

Harbison

2021

Genes

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Body fluid and body tissue identification are important in forensic science as they can provide key evidence in a criminal investigation and may assist the court in reaching conclusions. Establishing a link between identifying the fluid or tissue and the DNA profile adds further weight to this evidence. Many forensic laboratories retain techniques for the identification of biological fluids that have been widely used for some time. More recently, many different biomarkers and technologies have been proposed for identification of body fluids and tissues of forensic relevance some of which are now used in forensic casework. Here, we summarize the role of body fluid/ tissue identification in the evaluation of forensic evidence, describe how such evidence is detected at the crime scene and in the laboratory, elaborate different technologies available to do this, and reflect real life experiences. We explain how, by including this information, crucial links can be made to aid in the investigation and solution of crime.

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Discrimination of menstrual and peripheral blood traces using attenuated total reflection Fourier transform-infrared (ATR FT-IR) spectroscopy and chemometrics for forensic purposes

Cited by 27 publications

References 48 publications

Innovative Vibrational Spectroscopy Research for Forensic Application

Innovative Vibrational Spectroscopy Research for Forensic Application

Attenuated total reflection-Fourier transform infrared spectroscopy: a universal analytical technique with promising applications in forensic analyses

On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime

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