Discrimination of microbial diversity by fatty acid profiles of phospholipids and lipopolysaccharides in differently cultivated soils

Zelles, L.; Rackwitz, R.; Bai, Q.Y.; Beck, T.; Beese, F.

doi:10.1007/978-94-011-0479-1_9

Cited by 46 publications

(35 citation statements)

References 17 publications

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“…Treatments: ReF: rice paddies with fertilization; ReNF: rice paddies with no fertilization; VeF: vegetables with fertilization; VeNF: vegetables without fertilization. anaerobic bacteria [32], decreased significantly. Meanwhile, the abundance of a fungal biomarker, 18:1u9c, significantly increased, suggesting the converted vegetable fields favored fungal growth.…”

Section: Effect Of Land-use Conversion On Microbial Community Structurementioning

confidence: 91%

Responses of microbial community structure to land-use conversion and fertilization in southern China

Yuan

Dai

et al. 2015

European Journal of Soil Biology

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a b s t r a c tA short-term experiment was carried out in southern China to investigate the effects of land-use conversion from rice paddies to vegetable fields and fertilization on soil microbial community structure by analyzing soil phospholipid fatty acid (PLFA) profiles. A split-plot design with four replicates was adopted, in which land use (paddy and vegetable field) was the first-level treatment and fertilization (conventional fertilization and no fertilization) was nested as the second level. Our results showed that both land-use conversion and fertilization had significant effects on microbial community structure. After 2 years of land-use conversion, the total amount of PLFAs were 3.54 and 2.97 nmol g À1 for fertilized (VeF) and unfertilized (VeNF) vegetable fields, respectively, and 3.19 and 2.32 nmol g À1 for fertilized (R eF) and unfertilized (ReNF) rice paddies, respectively. Soil fungal PLFAs were 1.04 and 0.87 nmol g À1 for VeF and VeNF, respectively, which were significantly increased by 13.9 and 11.4 times compared with those of ReF and ReNF, respectively. The ratio of fungal to bacterial PLFAs significantly increased in vegetable fields compared with rice paddies. No significant differences were found in the total, bacterial, and actinomycetic PLFAs between vegetable fields and rice paddies. The application of fertilizer significantly increased the amount of total PLFAs and bacterial PLFAs. With land-use conversion and fertilization, soil physicochemical properties also changed, and microbial community structure showed a significant relationship with soil water content, NH 4 þ -N, and pH, which explained the land-use conversion and fertilization effects on soil microbial community composition.

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Section: Effect Of Land-use Conversion On Microbial Community Structurementioning

confidence: 91%

Responses of microbial community structure to land-use conversion and fertilization in southern China

Yuan

Dai

et al. 2015

European Journal of Soil Biology

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“…Phospholipid fatty acid extraction was performed according to Zelles et al (1995). Replicates from incubated SI soils with as well as respectively without methane (SI CH 4 , SI) were pooled and an equivalent of 10 g of dry soil were extracted with a mixture of 125 ml methanol, 63 ml chloroform and 50 ml phosphate buffer (0.05 M, pH 7).…”

Section: Phospholipid Fatty Acid Analysismentioning

confidence: 99%

Anaerobic oxidation of methane in grassland soils used for cattle husbandry

Bannert

Bogen²,

Esperschütz³

et al. 2012

Biogeosciences

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Abstract. While the importance of anaerobic methane oxidation has been reported for marine ecosystems, the role of this process in soils is still questionable. Grasslands used as pastures for cattle overwintering show an increase in anaerobic soil micro-sites caused by animal treading and excrement deposition. Therefore, anaerobic potential methane oxidation activity of severely impacted soil from a cattle winter pasture was investigated in an incubation experiment under anaerobic conditions using 13 C-labelled methane. We were able to detect a high microbial activity utilizing CH 4 as nutrient source shown by the respiration of 13 CO 2 . Measurements of possible terminal electron acceptors for anaerobic oxidation of methane were carried out. Soil sulfate concentrations were too low to explain the oxidation of the amount of methane added, but enough nitrate and iron(III) were detected. However, only nitrate was consumed during the experiment. 13 C-PLFA analyses clearly showed the utilization of CH 4 as nutrient source mainly by organisms harbouring 16 : 1ω7 PLFAs. These lipids were also found as most 13 Cenriched fatty acids by Raghoebarsing et al. (2006) after addition of 13 CH 4 to an enrichment culture coupling denitrification of nitrate to anaerobic oxidation of methane. This might be an indication for anaerobic oxidation of methane by relatives of "Candidatus Methylomirabilis oxyfera" in the investigated grassland soil under the conditions of the incubation experiment.

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“…The fatty acid nomenclature used was described by Frostegård et al (1993aFrostegård et al ( , 1993b. The branched, saturated PLFAs were chosen to represent Gram-positive bacteria (O'Leary and Wilkinson, 1988;Zelles et al, 1995). The monoenoic and cyclopropane unsaturated fatty acids and saturated fatty acids containing the OH group represented Gram-negative bacteria.…”

Section: Phospholipid Fatty Acid (Plfa) Analysismentioning

confidence: 99%

Plant growth and soil microbial community structure of legumes and grasses grown in monoculture or mixture

Chen

Marschner

2008

Journal of Environmental Sciences

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Discrimination of microbial diversity by fatty acid profiles of phospholipids and lipopolysaccharides in differently cultivated soils

Cited by 46 publications

References 17 publications

Responses of microbial community structure to land-use conversion and fertilization in southern China

Responses of microbial community structure to land-use conversion and fertilization in southern China

Anaerobic oxidation of methane in grassland soils used for cattle husbandry

Plant growth and soil microbial community structure of legumes and grasses grown in monoculture or mixture

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