Discrimination of psychrotolerant Bacillus cereus group based on MALDI-TOF MS analysis of ribosomal subunit proteins

Takahashi, Naomi; Nagai, Satomi; Fujita, Akane; Ido, Yousuke; Kato, Kenji; Saito, Ayumi; Moriya, Yuka; Tomimatsu, Yumiko; Kaneta, Naoko; Yoshinori, Tsujimoto; Tamura, Hiroto

doi:10.1016/j.fm.2020.103542

Cited by 17 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liquid chromatography–mass spectrometry (LC-MS) and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis are often used for the rapid detection of emetic Bacillus isolates in food products, by analyzing ribosomal subunit proteins [ 120 ] or targeting the distinct molecular of cereulide [ 70 , 121 , 122 , 123 , 124 ]. Cereulide is a cyclic molecule composed of a 36-membered ring and a small heat and acid stable cyclic dodecadepsipeptide of 1165 Da, which consist of alternating ester and amide bonds and the structure [- D -O-Leu- D -Ala- L -O-Val- L -Val-] 3 [ 23 ].…”

Section: Virulence Factors Of Bacillus Cereus Amentioning

confidence: 99%

“…Furthermore, another detection tool, Enzyme Immunoassay (EIA), is employed for the direct inspection on the protein level of the toxic components of B. cereus by targeting specific mAbs [117,118], which are able to purify toxins such as NheB and the NheB-C complex and neutralize the cytotoxicity [21,119]. Liquid chromatography-mass spectrometry (LC-MS) and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis are often used for the rapid detection of emetic Bacillus isolates in food products, by analyzing ribosomal subunit proteins [120] or targeting the distinct molecular of cereulide [70,[121][122][123][124]. Cereulide is a cyclic molecule composed of a 36membered ring and a small heat and acid stable cyclic dodecadepsipeptide of 1165 Da, which consist of alternating ester and amide bonds and the structure [-D-O-Leu-D-Ala-L-O-Val-L-Val-]3 [23].…”

Section: Detection Of Toxins Secreted From Bacillus Cereusmentioning

confidence: 99%

“…Currently, there is a large number of potential toxin genes related to diarrhea in these B. cereus strains, including the genes of hcbl, nhe, cytK and enterotoxin FM (entFM), as well as potential enterotoxins hlyII and enterotoxin T (BceT) [14]. As shown in Table 2, a study conducted in 10 local Liquid chromatography-mass spectrometry (LC-MS) and matrix assisted laser desorption/ ionization-time of flight (MALDI-TOF) analysis are often used for the rapid detection of emetic Bacillus isolates in food products, by analyzing ribosomal subunit proteins [120] or targeting the distinct molecular of cereulide [70,[121][122][123][124]. Cereulide is a cyclic molecule composed of a 36-membered ring and a small heat and acid stable cyclic dodecadepsipeptide of 1165 Da, which consist of alternating ester and amide bonds and the structure [-D -O-Leu-D -Ala-L -O-Val-L -Val-] 3 [23].…”

Section: Analysis Of Bacillus Toxin Detections In Dairy Products In Chinamentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Bacillus cereus in Dairy Products in China

Liu

et al. 2020

Toxins

View full text Add to dashboard Cite

Bacillus cereus is a common and ubiquitous foodborne pathogen with an increasing prevalence rate in dairy products in China. High and unmet demands for such products, particularly milk, raise the risk of B. cereus associated contamination. The presence of B. cereus and its virulence factors in dairy products may cause food poisoning and other illnesses. Thus, this review first summarizes the epidemiological characteristics and analytical assays of B. cereus from dairy products in China, providing insights into the implementation of intervention strategies. In addition, the recent achievements on the cytotoxicity and mechanisms of B. cereus are also presented to shed light on the therapeutic options for B. cereus associated infections.

show abstract

Section: Virulence Factors Of Bacillus Cereus Amentioning

confidence: 99%

Section: Detection Of Toxins Secreted From Bacillus Cereusmentioning

confidence: 99%

Section: Analysis Of Bacillus Toxin Detections In Dairy Products In Chinamentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Bacillus cereus in Dairy Products in China

Liu

et al. 2020

Toxins

View full text Add to dashboard Cite

show abstract

“…This has been described for both anaerobic bacteria and other genera, such as Bacillus spp. (Takahashi et al, 2020), Streptococcus spp. (Fan et al, 2017), Mycobacterium spp.…”

Section: Discussionmentioning

confidence: 99%

MALDI-TOF Mass Spectrometry Fingerprinting Performance Versus 16S rDNA Sequencing to Identify Bacterial Microflora From Seafood Products and Sea Water Samples

et al. 2021

View full text Add to dashboard Cite

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) associated with the Bruker BioTyperTM V7.0.0 database for the identification of 713 bacterial strains isolated from seafood products and sea water samples (ANSES B3PA collection) under culture conditions that may have been significantly different from those used to create the reference spectrum vs. the 16S rDNA sequencing. We identified 78.8% of seafood isolates with 46.7% at the species level (Bruker score above 2) and 21.2% (Bruker score between 1.7 and 2) at the genus level by the two identification methods, except for 3.8% of isolates with a difference of identification between the two methods (Bruker score between 1.7 and 2). There were 41.9% isolates (Bruker score below 1.7) with the identification at the genus level. We identified 94.4% of seafood isolates with 16S rDNA sequencing. The MALDI-TOF allowed a better strain identification to the species level contrary to the 16s rDNA sequencing, which allowed an identification mainly to the genus level. MALDI-TOF MS in association with the Bruker database and 16S rDNA sequencing are powerful tools to identify a wide variety of bacteria from seafood but require further identification by biochemical, molecular technique or other conventional tests.

show abstract

“…Psychrotolerant B. cereus group strains, such as B. weihenstephanensis and B. wiedmannii, can grow at 7 • C or below, whereas other B. cereus group strains cannot grow at less than 10 • C [3][4][5]. The psychrotolerant B. cereus strains can survive during the production, distribution, and storage of various refrigerated foods, such as dairy products [6]. As the psychrotolerant B. cereus group strains possess enterotoxin or emetic toxin genes [3,7], their existence in refrigerated food products should be monitored, and the control of these species is crucial for improving food safety.…”

Section: Introductionmentioning

confidence: 99%

Morphological Features and Cold-Response Gene Expression in Mesophilic Bacillus cereus Group and Psychrotolerant Bacillus cereus Group under Low Temperature

Park

Kim

et al. 2021

Microorganisms

View full text Add to dashboard Cite

At low temperatures, psychrotolerant B. cereus group strains exhibit a higher growth rate than mesophilic strains do. However, the different survival responses of the psychrotolerant strain (BCG34) and the mesophilic strain (BCGT) at low temperatures are unclear. We investigated the morphological and genomic features of BCGT and BCG34 to characterize their growth strategies at low temperatures. At low temperatures, morphological changes were observed only in BCGT. These morphological changes included the elongation of rod-shaped cells, whereas the cell shape in BCG34 was unchanged at the low temperature. A transcriptomic analysis revealed that both species exhibited different growth-related traits during low-temperature growth. The BCGT strain induces fatty acid biosynthesis, sulfur assimilation, and methionine and cysteine biosynthesis as a survival mechanism in cold systems. Increases in energy metabolism and fatty acid biosynthesis in the mesophilic B. cereus group strain might explain its ability to grow at low temperatures. Several pathways involved in carbohydrate mechanisms were downregulated to conserve the energy required for growth. Peptidoglycan biosynthesis was upregulated, implying that a change of gene expression in both RNA-Seq and RT-qPCR contributed to sustaining its growth and rod shape at low temperatures. These results improve our understanding of the growth response of the B. cereus group, including psychrotolerant B. cereus group strains, at low temperatures and provide information for improving bacterial inhibition strategies in the food industry.

show abstract

Discrimination of psychrotolerant Bacillus cereus group based on MALDI-TOF MS analysis of ribosomal subunit proteins

Cited by 17 publications

References 30 publications

Characterization of Bacillus cereus in Dairy Products in China

Characterization of Bacillus cereus in Dairy Products in China

MALDI-TOF Mass Spectrometry Fingerprinting Performance Versus 16S rDNA Sequencing to Identify Bacterial Microflora From Seafood Products and Sea Water Samples

Morphological Features and Cold-Response Gene Expression in Mesophilic Bacillus cereus Group and Psychrotolerant Bacillus cereus Group under Low Temperature

Contact Info

Product

Resources

About