Discrimination of suballeles present at the TNFd microsatellite locus using induced heteroduplex analysis

Spink, Charlotte F.; Keen, Leigh; Middleton, PG; Bidwell, J. L.

doi:10.1038/sj.gene.6364039

Cited by 7 publications

(13 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, as more knowledge is gained with regard to the gene maps of some of the cytokine genes, the influence on the polymorphisms in neighbouring genes and on the cytokine response may become more complex. For example, with regard to the TNF locus, a detailed analysis of this microsatellite locus has revealed additional genetic structures, SNPs and insertions/deletions that effectively define subtypes of the two candidate alleles TNFd3 and TNFd4, which have been implicated in the association studies in HSCT (Spink et al, 2004). In addition, IL-10 appears to possess a dual nature, with both pro-inflammatory and anti-inflammatory properties (Mocellin et al, 2003).…”

Section: Gene Associations With Hsct Outcome and Interpretation Of Rementioning

confidence: 99%

Genetic polymorphisms predicting the outcome of bone marrow transplants

et al. 2004

View full text Add to dashboard Cite

SummaryAnalysis of non‐histocompatibility leucocyte antigen (HLA) functional genomics, together with conventional risk factors in haematopoietic stem cell transplantation (HSCT) can lead to predicting outcome in HLA‐matched sibling transplant recipients. Polymorphisms of cytokine genes including tumour necrosis factor α, interleukin‐10, interferon γ and interleukin (IL)‐6, associate with more severe acute graft‐versus‐host disease (aGvHD). Donor genotype for IL‐1 receptor antagonist (IL‐1Ra) has been associated with reduced aGvHD severity. Other genotypes (patient IL‐1Ra, IL‐6 and donor IL‐1α) have been associated with chronic GvHD, or overall survival (Vitamin D receptor and oestrogen receptor). Polymorphisms within genes associated with host defence/inflammatory responses (mannose binding lectin genes, myeloperoxidase genes and the FCγ receptors) have been associated with infections. Polymorphisms of pharmacogenes, such as methylenetetrahydrofolate‐reductase, have been associated with aGvHD and other post‐transplant complications. The NOD2 gene polymorphism, associated with Crohn's disease, has been shown to be associated with risk of gut GvHD. The majority of the studies have been carried out in single centre HLA‐matched sibling cohorts and in relatively few matched unrelated donor transplants. This review gives an overall perspective of the current field of non‐HLA genetics with regard to HSCT outcome, clinical relevance and potential application of the results to clinical management of HSCT.

show abstract

Section: Gene Associations With Hsct Outcome and Interpretation Of Rementioning

confidence: 99%

Genetic polymorphisms predicting the outcome of bone marrow transplants

et al. 2004

View full text Add to dashboard Cite

show abstract

“…The STR in CMA1 is located 254 bp downstream of the gene (accession number BV210164) [20]. The STR in IL-4 is located in intron 3 (VE6523) of the gene [37] and the STR of the TNFd locus is located 8 kb downstream of the TNF-␣ gene in intron 3 of the LST-1 gene [44].…”

Section: Genetic Analysismentioning

confidence: 99%

Association of STR polymorphisms in CMA1 and IL-4 with asthma and atopy: The SAPALDIA Cohort

et al. 2010

View full text Add to dashboard Cite

“…Thus, genotypic diagnosis is verified by two independent ways. Previous study of microsatellites has shown that patterns of HA can achieve an accurate allelic assignation [19], whereas other investigations have revealed patterns that can be very complex [20,23], making genotyping categorization difficult. In the present study, the different heteroduplexes originating from different MsH43 alleles ensure a reliable genotypic assignment.…”

mentioning

confidence: 96%

“…The HA has been used for detecting and characterizing allelic variation and mutations, particularly those related to human inherited genetic disorders [15,16]. This approach was used for typing human lymphocyte antigen [17] and microsatellite loci [18][19][20].…”

mentioning

confidence: 99%

Heteroduplex analysis of minisatellite variability

Barros¹,

Blanco²,

Boán³

et al. 2005

Electrophoresis

View full text Add to dashboard Cite

Minisatellites are tandem repeat arrays of middle size (5-100 bp) repeat units widely distributed in eukaryotic genomes. They have been related to several important features of human genome biology, including gene regulation, chromosomal fragile sites, and imprinting. In this report, we have critically assessed and employed heteroduplex analysis (HA) for the identification of different human minisatellite MsH43 alleles. This minisatellite is organized as a repeat array of 5-6 bp units spanning 0.5 kbp. Our results demonstrate that this procedure is an easy, rapid, and reliable method to document allelic diversity for this locus. This work suggests that HA will also be a useful tool for studying the polymorphism of other minisatellites with small repeat units.

show abstract

Discrimination of suballeles present at the TNFd microsatellite locus using induced heteroduplex analysis

Cited by 7 publications

References 21 publications

Genetic polymorphisms predicting the outcome of bone marrow transplants

Genetic polymorphisms predicting the outcome of bone marrow transplants

Association of STR polymorphisms in CMA1 and IL-4 with asthma and atopy: The SAPALDIA Cohort

Heteroduplex analysis of minisatellite variability

Contact Info

Product

Resources

About