Discrimination of the variety and region of origin of extra virgin olive oils using 13C NMR and multivariate calibration with variable reduction

Shaw, Adrian D.; Camillo, Angela Di; Vlahov, Giovanna; Jones, A. R.; Bianchi, Giorgio; Rowland, Jem J.; Kell, Douglas B.

doi:10.1016/s0003-2670(97)00037-8

Cited by 95 publications

(86 citation statements)

References 56 publications

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“…[6 -8] 1 H NMR spectroscopy has been used in the analysis of olive oil for measuring diglyceride content, determinations of squalene, cyclo-arthenol and Mg-depleted chlorophyll, and analysis of sterols as well as other components responsible for the taste and aroma of olive oil such as acetic acid, trans-2-hexenal and formaldehyde. [1,2] Although GC (gas chromatography) is the most commonly used technique for the qualitative and quantitative determination of fatty acid residues in vegetable oils, 13 C NMR spectroscopy can also be used for analyzing these major components. [1 -5] The distinct advantage of using 13 C NMR spectroscopy in this context is that no chemical derivatization of the sample is required whereas for GC the fatty acid methyl esters must be prepared from the triacylglycerols before analysis is undertaken.…”

Section: Introductionmentioning

confidence: 99%

“…[1,2] Although GC (gas chromatography) is the most commonly used technique for the qualitative and quantitative determination of fatty acid residues in vegetable oils, 13 C NMR spectroscopy can also be used for analyzing these major components. [1 -5] The distinct advantage of using 13 C NMR spectroscopy in this context is that no chemical derivatization of the sample is required whereas for GC the fatty acid methyl esters must be prepared from the triacylglycerols before analysis is undertaken. 13 C NMR spectroscopy has been successfully used to determine quantitatively the major fatty acid residues in olive oils [5,7] and moreover can give direct information about the positional distribution of the fatty acids on the glycerol backbone.…”

Section: Introductionmentioning

confidence: 99%

“…[1 -5] The distinct advantage of using 13 C NMR spectroscopy in this context is that no chemical derivatization of the sample is required whereas for GC the fatty acid methyl esters must be prepared from the triacylglycerols before analysis is undertaken. 13 C NMR spectroscopy has been successfully used to determine quantitatively the major fatty acid residues in olive oils [5,7] and moreover can give direct information about the positional distribution of the fatty acids on the glycerol backbone. Much success has been found in detecting adulteration of olive oil by other oils using 13 C NMR spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

“…13 C NMR spectroscopy has been successfully used to determine quantitatively the major fatty acid residues in olive oils [5,7] and moreover can give direct information about the positional distribution of the fatty acids on the glycerol backbone. Much success has been found in detecting adulteration of olive oil by other oils using 13 C NMR spectroscopy. In conjunction with chemometric methods 13 C NMR spectroscopy can also be used to distinguish between geographic and cultivar-based differences of olive oils.…”

Section: Introductionmentioning

confidence: 99%

“…Much success has been found in detecting adulteration of olive oil by other oils using 13 C NMR spectroscopy. In conjunction with chemometric methods 13 C NMR spectroscopy can also be used to distinguish between geographic and cultivar-based differences of olive oils. [1 -3,9] We have recently become interested in the analysis of other high-value vegetable oils, including apricot kernel, avocado pear, grapeseed, macadamia nut, mango kernel and marula oils.…”

Section: Introductionmentioning

confidence: 99%

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A novel approach to the rapid assignment of ¹³C NMR spectra of major components of vegetable oils such as avocado, mango kernel and macadamia nut oils

Retief

McKenzie²,

Koch

2009

Magnetic Reson in Chemistry

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Assignment of 13 C nuclear magnetic resonance (NMR) spectra of major fatty acid components of South African produced vegetable oils was attempted using a method in which the vegetable oil was spiked with a standard triacylglycerol. This proved to be inadequate and therefore a new rapid and potentially generic graphical linear correlation method is proposed for assignment of the 13 C NMR spectra of major fatty acid components of apricot kernel, avocado pear, grapeseed, macadamia nut, mango kernel and marula vegetable oils. In this graphical correlation method, chemical shifts of fatty acids present in a known standard triacylglycerol is plotted against the corresponding chemical shifts of fatty acids present in the vegetable oils. This new approach (under carefully defined conditions and concentrations) was found especially useful for spectrally crowded regions where significant peak overlap occurs and was validated with the well-known 13 C NMR spectrum of olive oil which has been extensively reported in the literature. In this way, a full assignment of the 13 C{1H} NMR spectra of the vegetable oils, as well as tripalmitolein was readily achieved and the resonances belonging to the palmitoleic acid component of the triacylglycerols in the case of macadamia nut and avocado pear oil resonances were also assigned for the first time in the 13 C NMR spectra of these oils.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A novel approach to the rapid assignment of ¹³C NMR spectra of major components of vegetable oils such as avocado, mango kernel and macadamia nut oils

Retief

McKenzie²,

Koch

2009

Magnetic Reson in Chemistry

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Variable selection and multivariate methods for the identification of microorganisms by flow cytometry

et al. 1999

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Background: When exploited fully, flow cytometry can be used to provide multiparametric data for each cell in the sample of interest. While this makes flow cytometry a powerful technique for discriminating between different cell types, the data can be difficult to interpret. Traditionally, dual‐parameter plots are used to visualize flow cytometric data, and for a data set consisting of seven parameters, one should examine 21 of these plots. A more efficient method is to reduce the dimensionality of the data (e.g., using unsupervised methods such as principal components analysis) so that fewer graphs need to be examined, or to use supervised multivariate data analysis methods to give a prediction of the identity of the analyzed particles. Materials and Methods: We collected multiparametric data sets for microbiological samples stained with six cocktails of fluorescent stains. Multivariate data analysis methods were explored as a means of microbial detection and identification. Results: We show that while all cocktails and all methods gave good accuracy of predictions (>94%), careful selection of both the stains and the analysis method could improve this figure (to >99% accuracy), even in a data set that was not used in the formation of the supervised multivariate calibration model. Conclusions: Flow cytometry provides a rapid method of obtaining multiparametric data for distinguishing between microorganisms. Multivariate data analysis methods have an important role to play in extracting the information from the data obtained. Artificial neural networks proved to be the most suitable method of data analysis. Cytometry 35:162–168, 1999. © 1999 Wiley‐Liss, Inc.

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Adulteration of Olive Oil

Mossoba

Srigley

Karunathilaka

et al. 2020

Bailey's Industrial Oil and Fat Products

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The US Food and Drug Administration (US FDA) is mandated with protection of the US public against intentional adulteration of foods for economically motivated gain and deceptive label declarations such as those found with adulterated extra virgin olive oil (EVOO). In April 2016, a US Congressional Committee expressed concerns related to reports of the high prevalence of imported olive oil sold in the United States that is adulterated or mislabeled. Such fraudulence reportedly includes the mixing of EVOO with seed oil, which could adversely impact the health of consumers who are allergic to seed oil. The most serious issue facing regulatory agencies has been the relative ease of adulterating EVOO while meeting the physical and chemical criteria of established trade standards, such as those of the International Olive Council (IOC) and the US Department of Agriculture (USDA). This issue underscores the urgent need for more rigorous and accurate analytical methodologies, including untargeted rapid screening tools, for detecting such fraudulence. This article presents an overview of current official methods, analytical procedures that target specific analytes, and rapid screening tools that rely on chemometric analysis.

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Discrimination of the variety and region of origin of extra virgin olive oils using 13C NMR and multivariate calibration with variable reduction

Cited by 95 publications

References 56 publications

A novel approach to the rapid assignment of ¹³C NMR spectra of major components of vegetable oils such as avocado, mango kernel and macadamia nut oils

A novel approach to the rapid assignment of ¹³C NMR spectra of major components of vegetable oils such as avocado, mango kernel and macadamia nut oils

Variable selection and multivariate methods for the identification of microorganisms by flow cytometry

Adulteration of Olive Oil

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Discrimination of the variety and region of origin of extra virgin olive oils using 13C NMR and multivariate calibration with variable reduction

Cited by 95 publications

References 56 publications

A novel approach to the rapid assignment of 13C NMR spectra of major components of vegetable oils such as avocado, mango kernel and macadamia nut oils

A novel approach to the rapid assignment of 13C NMR spectra of major components of vegetable oils such as avocado, mango kernel and macadamia nut oils

Variable selection and multivariate methods for the identification of microorganisms by flow cytometry

Adulteration of Olive Oil

Contact Info

Product

Resources

About

A novel approach to the rapid assignment of ¹³C NMR spectra of major components of vegetable oils such as avocado, mango kernel and macadamia nut oils

A novel approach to the rapid assignment of ¹³C NMR spectra of major components of vegetable oils such as avocado, mango kernel and macadamia nut oils