Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

Avci, Ertuğ; Kaya, Nur Selin; Ucankus, Gizem; Çulha, Mustafa

doi:10.1007/s00216-015-8950-5

Cited by 27 publications

(37 citation statements)

References 49 publications

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“…In the third approach used to obtain SERS spectra of bacteria, bacteria and colloid are mixed together [ 38 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 ]. Some researchers would obtain SERS spectra of the bacteria-colloid suspension.…”

Section: Resultsmentioning

confidence: 99%

“…Spectral features observed in SERS spectra are dependent upon the growth phase of the bacteria [ 55 , 71 ]. One study placed harvested bacteria on Ag/AAO substrates [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

“…One study placed harvested bacteria on Ag/AAO substrates [ 55 ]. Another study mixed bacteria with hydroxylamine-generated Ag NPs that were then placed on the surface of CaF 2 slides [ 71 ]. Because both studies used uncapped Ag NPs, SERS spectra were dominated by cell metabolic secretions, discussed in Section 2.2.2 and Section 2.2.3 .…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Review on SERS of Bacteria

Mosier‐Boss¹

2017

Biosensors

121

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Surface enhanced Raman spectroscopy (SERS) has been widely used for chemical detection. Moreover, the inherent richness of the spectral data has made SERS attractive for use in detecting biological materials, including bacteria. This review discusses methods that have been used to obtain SERS spectra of bacteria. The kinds of SERS substrates employed to obtain SERS spectra are discussed as well as how bacteria interact with silver and gold nanoparticles. The roll of capping agents on Ag/Au NPs in obtaining SERS spectra is examined as well as the interpretation of the spectral data.

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Section: Resultsmentioning

confidence: 99%

“…Spectral features observed in SERS spectra are dependent upon the growth phase of the bacteria [ 55 , 71 ]. One study placed harvested bacteria on Ag/AAO substrates [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

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Review on SERS of Bacteria

Mosier‐Boss¹

2017

Biosensors

121

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“…Pitris and Ziegler group reported a few proceedings of SERS for classifying bacterial samples as positive or negative for UTI and their antibiotic sensitivities [ 18 , 19 ]. As reported by Avci et al and Mircescu et al , UTI causative bacterial species at their different growth phases could be discriminated [ 20 , 21 ]. The SERS detection of bacteria from complex samples usually needed an enrichment such as filtration and centrifugation or using the help of magnetic nanoparticles (NPs) [ 22 ], which takes most time of analytical procedure.…”

Section: Introductionmentioning

confidence: 99%

Portable bacteria-capturing chip for direct surface-enhanced Raman scattering identification of urinary tract infection pathogens

et al. 2018

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Acute urinary tract infections (UTIs) are one of the most common nosocomial bacterial infections, which affect almost 50% of the population at least once in their lifetime. UTIs may lead to lethal consequences if they are left undiagnosed and not properly treated. Early, rapid and accurate uropathogens detection methods play a pivotal role in clinical process. In this work, a portable bacteria-grasping surface-enhanced Raman scattering (SERS) chip for identification of three species of uropathogens (Escherichia coli CFT 073, Pseudomonas aeruginosa PAO1 and Proteus mirabilis PRM1) directly from culture matrix was reported. The chip was firstly modified with a positively charged NH3+ group, which enables itself grasp the negatively charged bacterial cells through the electrostatic adsorption principle. After the bacterial cells were captured by the chip, concentrated Ag nanoparticles (NPs) were used to obtain their Raman fingerprint spectra with recognizable characteristic peaks and good reproducibility. With the help of chemometric method such as discriminant analysis (DA), the SERS-based chip allows a rapid, successful identification of three species of UTI bacteria with a minimal bacterial concentration (105 cells ml−1) required for clinical diagnostics. In addition, this chip could spot the bacterial SERS fingerprints information directly from LB culture medium and artificial urine without sample pre-treatment. The portable bacteria-grasping SERS-based chip provides a possibility for fast and easy detection of uropathogens, and viability of future development in healthcare applications.

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“…UTI‐causing bacteria were discriminated by differences in their growth profiles . Here, the researchers tracked the temporal fluctuations in the SERS spectra of UTI‐causing bacteria.…”

Section: Sers‐based Detection Of Antibiotic‐resistant Bacteriamentioning

confidence: 99%

Surface‐Enhanced Raman Scattering for Rapid Detection and Characterization of Antibiotic‐Resistant Bacteria

Galvan

2018

Adv Healthcare Materials

105

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As the prevalence of antibiotic-resistant bacteria continues to rise, biosensing technologies are needed to enable rapid diagnosis of bacterial infections. Furthermore, understanding the unique biochemistry of resistance mechanisms can facilitate the development of next generation therapeutics. Surface-enhanced Raman scattering (SERS) offers a potential solution to real-time diagnostic technologies, as well as a route to fundamental, mechanistic studies. In the current review, SERS-based approaches to the detection and characterization of antibiotic-resistant bacteria are covered. The commonly used nanomaterials (nanoparticles and nanostructured surfaces) and surface modifications (antibodies, aptamers, reporters, etc.) for SERS bacterial detection and differentiation are discussed first, and followed by a review of SERS-based detection of antibiotic-resistant bacteria from environmental/food processing and clinical sources. Antibiotic susceptibility testing and minimum inhibitory concentration testing with SERS are then summarized. Finally, recent developments of SERS-based chemical imaging/mapping of bacteria are reviewed.

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Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

Cited by 27 publications

References 49 publications

Review on SERS of Bacteria

Review on SERS of Bacteria

Portable bacteria-capturing chip for direct surface-enhanced Raman scattering identification of urinary tract infection pathogens

Surface‐Enhanced Raman Scattering for Rapid Detection and Characterization of Antibiotic‐Resistant Bacteria

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