The SIMENDO is an affordable virtual reality simulator designed to train basic psychomotor skills for endoscopic surgery. This study aimed first to establish construct validity by determining which parameters can discriminate groups with different experience levels, and second to establish the extent to which training is useful by determining when inexperienced groups reach expert level. Methods: The study participants were divided into four groups according to their experience with endoscopic procedures: experienced group (group A, >50 procedures performed, n = 15), intermediate group (group B, 1-50 procedures performed, n = 18), endoscope navigation group (group C, endoscope navigation experience, n = 14), and novice group (group D, no endoscopic experience, n = 14). Each participant performed three repetitions of six consecutive exercises. The parameters studied were task time, path length of the instruments, and number of errors (collisions). Some participants continued training up to 10 repetitions to get insight in the learning curve. Results: Group A (expert) outperformed all the other groups (B, C, and D) in terms of total median task time (p < 0.05), groups C and D in terms of path length, and group D in terms of collision frequency in the first two repetitions. Group B (intermediate) outperformed group D (novice) in total time and endoscope path length for all repetitions, and group C (camera navigation) outperformed group D (novice) in the first repetition. Less experienced groups D and C did not reach expert level for the task time within 10 repetitions, and group B reached it after the eighth repetition (p < 0.05). Conclusion: The study was able to establish construct validity for the training program with the simulator under study. The learning curve showed that training with this simulator is useful for subjects with or without limited endoscopic experience. Furthermore, previous endoscopic camera navigation already improves motor skills to more than the basic level.Key words: Construct validity -LaparoscopyMotor skills -Simulator -Training -Virtual reality Endoscopic virtual reality (VR) trainers have become an attractive and valuable tool for training surgeons in a nonpatient environment. The aim of a simulator is to shorten the learning curve of the trainee for the real job.The SIMENDO (DelltaTech, Delft, The Netherlands) is a recently developed affordable simulator (5-8 thousand euro for software and hardware) aimed at training hand-eye coordination motor skills needed to perform endoscopic surgery. This simulator is designed to provide an easy-to-use plug-and-play system for surgical trainees. It features abstract tasks and simultaneously measures the performance of subjects using various parameters for objective assessment. However, for practical and effective use in the surgical curriculum, tasks and metrics incorporated in the simulator need to be tested for objectivity and reliability. Consequently, each new training device must be assessed and validated.In the literature, the validation ...