Disease resistance and response against Vibrio anguillarum intestinal infection in European seabass ( Dicentrarchus labrax ) fed low fish meal and fish oil diets

Torrecillas, Silvia; Caballero, M. J.; Mompel, D.; Montero, Daniel; Zamorano, M. J.; Robaina, L.; Rivero-Ramírez, F.; Karalazos, Vasileios; Kaushik, Sadasivam; Izquierdo, M.S.

doi:10.1016/j.fsi.2017.06.022

Cited by 43 publications

(19 citation statements)

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“…Indeed, juvenile fish of gilthead sea bream are able to grow fast and efficiently from early life stages until market size with practical diets containing <10% marine feed ingredients (Benedito‐Palos et al., ). However, carnivorous fish fed with little or no fish meal/fish oil diets have been shown to have a wide range of metabolic effects that, in addition to affecting growth performance, can also impact on health (Martin & Król, ; Torrecillas et al., ) or the nutritious value of seafood products as the most important source of omega‐3 in the human diet (Ballester‐Lozano, Benedito‐Palos, Mingarro, Navarro, & Pérez‐Sánchez, ; Ballester‐Lozano et al., ; Benedito‐Palos et al., ). The precise mechanisms for this disruption of fish health and welfare are not fully understood, although recent studies with extreme diet formulations pointed out in gilthead sea bream that dietary supplementation of plant‐based diets with sodium butyrate helps to prevent in long‐term feeding trials the inflammation of the intestinal epithelium, preserves the integrity of the intestinal barrier (Estensoro et al., ) and improves bacteria and parasite disease outcomes (Piazzon et al., , ).…”

Section: Plant‐based Diets Experimental Set‐up and Data Collectionmentioning

confidence: 99%

Comprehensive overview of feed‐to‐fillet transfer of new and traditional contaminants in Atlantic salmon and gilthead sea bream fed plant‐based diets

et al. 2018

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European fish farming drives the development of sustainable marine fish feed where marine ingredients are replaced by alternatives such as plant meals and vegetable oils. A new scenario emerges where noncurrent and/or new contaminants enter into the fish food chain as potential food safety and/or welfare concerns. Novel analytical methodologies must be developed to assess the large variety of contaminants from different families in feed ingredients, feeds based on these ingredients and fish farmed on the novel feeds. Of the novel contaminants identified in feed ingredients and feeds, the pesticides, such as chlorpyrifos‐methyl, pirimiphos‐methyl, boscalid and azoxystrobin, and the mycotoxins, such as deoxynivalenol and fumonisins (B1 + B2 + B3), were detected. Polyaromatic hydrocarbons, especially those with ≤4 ring, were found in all samples. Information of the transfer of contaminants from feed to public foodstuffs is essential for an appropriate human risk assessment of feed contaminants as well as for the harmonization of legislation of contaminants throughout the aquaculture production chain. However, the pesticides and mycotoxins found in feed were not detected in fish fillet, and hence, no transfer factor could be established. As a result, no apparent risk seems to be associated with these contaminants in fish fillets at the current background exposure levels by feeds.

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Section: Plant‐based Diets Experimental Set‐up and Data Collectionmentioning

confidence: 99%

Comprehensive overview of feed‐to‐fillet transfer of new and traditional contaminants in Atlantic salmon and gilthead sea bream fed plant‐based diets

et al. 2018

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“…It has been extensively proven that the inclusion of alternative plant proteins can lead to nutritional imbalances [ 23 , 31 ] and immune dysfunctions [ 24 , 32 ], particularly in carnivorous fish, increasing their susceptibility to pathogenic invasion, disease, and finally, death. The purpose of this work was to confirm, in ex vivo conditions, the effect caused by total FM substitution for a long and short period on immune intestinal status.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, lower gene expression values reported in the FM group might be related to a higher protection in the host from bacterial adhesion and growth. Nevertheless, results should be analyzed with caution, since a wide individual variation of inflammatory and immune genes expression has been reported in other species [ 61 ] and the level of expression before the ex vivo trial conditions the inflammatory and immune capacity registered after bacterial exposure [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Peñaranda

Bäuerl

Tomás‐Vidal

et al. 2020

IJMS

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The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in the study of the interactions between pathogenic bacteria, rearing conditions, or dietary components and fish gut immune response. The objective of this study was to determine the influence of the total substitution of fish meal by plants on the immune intestinal status of seabream using an ex vivo bacterial challenge. For this aim, two growth stages of fish were assayed (12 g): phase I (90 days), up to 68 g, and phase II (305 days), up to 250 g. Additionally, in phase II, the effects of long term and short term exposure (15 days) to a plant protein (PP) diet were determined. PP diet altered the mucosal immune homeostasis, the younger fish being more sensitive, and the intestine from fish fed short-term plant diets showed a higher immune response than with long-term feeding. Vibrio alginolyticus (V. alginolyticus) triggered the highest immune and inflammatory response, while COX-2 expression was significantly induced by Photobacterium damselae subsp. Piscicida (P. damselae subsp. Piscicida), showing a positive high correlation between the pro-inflammatory genes encoding interleukin 1β (IL1-β), interleukin 6 (IL-6) and cyclooxygenase 2(COX-2).

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“…It has been extensively proven that the inclusion of alternative plant proteins can lead to nutritional imbalances [23,40] and immune dysfunctions [24,41], particularly in carnivorous sh, increasing their susceptibility to pathogenic invasion, disease and nally death. Findings of present study suggest that the sh gut response to the total dietary substitution of sh meal by plant protein meals can differ at short term and long-term feeding and the sh size.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, lower gene expression values reported in FM group might be related to a higher protection in host from bacterial adhesion and growth. Nevertheless, results should be analysed with caution since a wide individual variation of in ammatory and immune genes expression has been reported in other species [70] and level of expression before the ex vivo trial conditions the in ammatory and immune capacity registered after bacterial exposure [41].…”

Section: Discussionmentioning

confidence: 99%

Intestinal Explant Cultures from Gilthead Seabream (Sparus Aurata, L.) Allowed Determining the Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Peñaranda

Bäuerl

Tomás‐Vidal

et al. 2020

Preprint

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BackgroundAlthough high levels of fish meal replacement by alternative protein sources have been achieved without relevant alterations in terms of growth performance, negative effects on immune status were detected. The diet and fish immunity interactions at gut level have been widely discussed, although in vivo approaches have reported several limitations. In this sense, intestine explant culture system can be a valuable complementary tool to study the interactions between pathogenic bacteria and fish gut response, and the possible influence of environmental, breeding, rearing conditions or dietary components on the responsiveness of the innate immune system in fish.The object of this study was to test the impact of total substitution of fish meal by plant protein on the intestinal health of seabream (12g) in two growth stages: phase I (90 days), up to 68 g, and phase II (305 days), up to 250g. In phase II, the effects of the long term and short exposure (15 days) to plant protein diet were determined. In order to determine the effect of plant protein feeding on the innate immune response to bacterial pathogens, and an ex vivo procedure of intestine explants culture was implemented.ResultsFish showed less tolerance to dietary plant protein in phase I than in phase II, while the ex vivo assays indicated that the intestine from fish fed at short-term plant diets showed a higher immune response than at long term feeding.In relation to the immune response to bacterial challenge, a significant expression in pro-inflammatory cytokines IL-1β and IL-6 after 6 hours of exposure to V. algynoliticus, while COX-2 expression was significantly induced by P. damselae subsp. pisicida, showing positive high correlation between them.ConclusionsA differential health status was observed depending of growth stage, being stricter to the plant protein inclusion the younger fish. The new experimental system based on fish intestinal explants culture has been successfully implemented, becoming an effective methodology for ex vivo studies. Under ex vivo conditions, the bacterial challenge induced inflammatory and immune intestinal response, responding stronger those intestine of fish fed during a short-term with a total substitution of fish meal.

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Disease resistance and response against Vibrio anguillarum intestinal infection in European seabass ( Dicentrarchus labrax ) fed low fish meal and fish oil diets

Cited by 43 publications

References 50 publications

Comprehensive overview of feed‐to‐fillet transfer of new and traditional contaminants in Atlantic salmon and gilthead sea bream fed plant‐based diets

Comprehensive overview of feed‐to‐fillet transfer of new and traditional contaminants in Atlantic salmon and gilthead sea bream fed plant‐based diets

Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Intestinal Explant Cultures from Gilthead Seabream (Sparus Aurata, L.) Allowed Determining the Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

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