Disease Resistance in Potato Haploids

Jansky, S. H.; Mauritz, E.D.; Clark, J. J.; Rouse, D. I.

doi:10.17660/actahortic.2003.619.14

Cited by 4 publications

(2 citation statements)

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“…Hence, taking into consideration the DSI of 'PAJ' and its diploid progenitor 'PMM', in 'PAJ', as a result of the elimination of two sets of genomes (13 X 2 = 26) of 'PMM', it is assumed that certain loci responsible for resistance/susceptibility to the pathogen or whole genomes are up-or down-regulated, which in turn might have resulted in remarkable increase in resistance response for the pathogen, as evident from its signi cantly low DSI compared to 'PMM'. Jansky et al (2003) have reported higher levels of disease resistance in haploid potato clones than their tetraploid parents.…”

Section: Resultsmentioning

confidence: 99%

Significance of ploidy on par with genotypic specificity in host resistance for Phytophthora in Black pepper (Piper nigrum L.)

Jacob,

Davis,

Jabbar

2024

Preprint

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We estimated Disease Severity Indices (DSI) of 22 genotypes of Black pepper by subjecting them to screening against Phytophthora capsici for assessing the influence of ploidy as well as genotype in host resistance. The DSI of the genotypes showed significant variation and established the existence of genotypic specificity in host resistance in Black pepper. ‘PAJ’, a haploid genotype of the crop, exhibited the highest resistance to the pathogen, as evident by its lowest DSI (33.33 %), and rated it as ‘moderately resistant’. Its diploid progenitor ‘PMM’ and all the other diploid genotypes were rated as ‘susceptible’, since their DSI were > 40 %. ‘PAJ’ is genetically a product of haplodisation of ‘PMM’, and it showed significantly higher resistance than ‘PMM’ for the pathogen. Hence, it is inferred that ploidy has remarkable influence in Black pepper – Phytophthorapathosystem. This study is insightful on the possible genomic interventions for augmenting the inherent degree of resistance exhibited by the genotypes of the crop for the pathogen. Therefore, outcome of this study is a breakthrough, which can transform the present-day haphazard, less efficient approach in resistance breeding in Black pepper for Phytophthora foot rot to one which is systematic and efficient.

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Section: Resultsmentioning

confidence: 99%

Significance of ploidy on par with genotypic specificity in host resistance for Phytophthora in Black pepper (Piper nigrum L.)

Jacob,

Davis,

Jabbar

2024

Preprint

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“…Dihaploids are valuable for revealing both the positive and negative genetic variation hidden in their tetraploid parents (Peloquin and Hougas 1960;Kotch 1987). For example, dihaploids with levels of disease resistance higher than their tetraploid parents have been identified (Cipar and Lawrence 1972;Jansky et al 2003;Ercolano et al 2004).…”

Section: Introductionmentioning

confidence: 99%

A High Throughput Method for Generating Dihaploids from Tetraploid Potato

et al. 2021

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There is a worldwide effort to increase the efficiency of potato (Solanum tuberosum L.) cultivar development by using inbred diploid breeding lines. This activity is impeded by the cost and effort required to produce dihaploids from cultivated tetraploid potatoes. We developed a high throughput dihaploid production method based on the 60-year-old method of Peloquin and Hougas. Red Norland inflorescences from commercial fields were transferred to greenhouses. As buds developed, pollen from the dihaploid inducer IVP 101 was applied systematically to thousands of stigmas per trial. Berries were harvested 21 days after pollination. Seeds of putative dihaploids lacking a seed spot marker were retained and ploidy was confirmed using flow cytometry. We recovered 23 dihaploids from 21,651 pollinations. This is a promising method for systematically carrying out thousands of pollinations since the cost of field-grown flowers is dramatically less than that of greenhouse-grown flowers.

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