Diseases of Blood

Priya, C. K. Vishnu; Devi, Mutum Sangeeta; Niveditha, B.; Poireiton, Athokpam; Anupriya, Ch.; Ranjan, Ravi; Poorni, .; Lakshminrusimhan, DKS

doi:10.9734/bpi/mono/978-93-5547-990-7

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…21 Using a distinctly different approach, CCS measurements have also been realized through analysis of the decay rate of the time domain signal from FT mass analyzers. 24,25 Using either the width of frequency domain peaks [26][27][28][29][30] or direct analysis of the time domain transient signal, 31 the CCS of small molecules, 26,32,33 peptides, 28,30 and intact proteins 30,31 have been measured on Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers. CCS measurements of small molecules and peptides have also been performed in charge detection mass spectrometers 34 and electrostatic linear ion traps.…”

Section: Introductionmentioning

confidence: 99%

Expanding Orbitrap collision cross section measurements to native protein applications through kinetic energy and signal decay analysis

James

Sanders

Fort

et al. 2022

Preprint

View full text Add to dashboard Cite

The measurement of collision cross sections (CCS) offers supplemental information about sizes and conformations of ions beyond mass analysis alone. We have previously shown that CCSs can be determined directly from the time-domain transient decay of ions in an Orbitrap mass analyzer as ions oscillate around the central electrode and collide with neutral gas, thus removing them from the ion packet. Herein, we develop the soft sphere collision model, thus deviating from prior FT-MS CCS hard sphere model, to determine CCSs as a function of center-of-mass collision energy in the Orbitrap analyzer. With this model, we aim to increase the upper mass limit of CCS measurement for native-like proteins, characterized by low charge states and presumed to be in more compact conformations. We also combine CCS measurements with collision inducing unfolding and MS/MS experiments to monitor protein unfolding and disassembly of protein complexes and measure CCSs of ejected monomers from protein complexes.

show abstract