This paper describes the design and development of a Graphical User Interface through the virtual instrumentation software NI LabVIEW using the VISA function, to graphically visualize and storage the data of the climatological variables of temperature and relative humidity. The graphical interface offers the option to export the date, time and data of the two variables to text documents with extension “.txt”, which acquires the information of the electronic board wireless monitoring and control, which uses a main device PIC16F877A microcontroller. AMT1001 Precision Analog Sensor was used to sense temperature and relative humidity. The PIC16F877A was programmed using a C programming language in the CCS Compiler compiler, to the data acquisition, and send it via RS232 communication to the computer, using the PL2303 module USB to TTL converter. To check the GUI operation, the electronic wireless monitoring and control card was connected to the computer equipment by wire, however, the monitoring of the climate variables can be done wirelessly by XBEE technology. Future work aims to monitor the climate of a horticultural greenhouse with XBBE technology, so that the data is sent wirelessly to a computer that has the GUI, and is also connected to Ethernet or WIFI, which will have the LabVIEW graphical interface explained in this article, and the data will be displayed / analyzed through the internet.