Disentangling survival of Escherichia coli O157:H7 in soils: From a subpopulation perspective

Han, Ziming; Huang, Guannan; Liao, Jiafen; Li, Jiahang; Lyu, Guangze; Ma, Jincai

doi:10.1016/j.scitotenv.2020.141649

Cited by 13 publications

(8 citation statements)

References 52 publications

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“…The decay characteristics at the storage temperatures were analyzed with the GInaFiT Version 1.7 (Geeraerd and Van Impe Inactivation Model Fitting Tool) freeware add-in for Microsoft® Office 365® Excel ( Geeraerd et al, 2005 ), as done earlier ( Tiwari et al, 2019 ; Han et al, 2020 ). GInaFiT is a commonly used tool for modeling the decay of microbes or their genetic markers.…”

Section: Methodsmentioning

confidence: 99%

The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland

Hokajärvi

Rytkönen

Tiwari

et al. 2021

Science of The Total Environment

141

104

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Section: Methodsmentioning

confidence: 99%

The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland

Hokajärvi

Rytkönen

Tiwari

et al. 2021

Science of The Total Environment

141

104

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“…The optimal sequence length of the target fragment was 280-450 bp. Fungal primer sequences were designed and reported in previous studies [ 14 , 15 ]. The universal primer sequences used were ITS5-1737F: 5′-GGAAGTAAA AGTCGTAACAAGG-3′ and ITS2-2043R: 5′-GCTGCGTTCTTCATCGATGC-3′.…”

Section: Methodsmentioning

confidence: 99%

Dysbiosis of Gastric Mucosal Fungal Microbiota in the Gastric Cancer Microenvironment

Zhang

Feng

Qiu

et al. 2022

Journal of Immunology Research

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Background. Microbes have been shown to contribute to gastric cancer (GC), gastric bacteria and viruses are associated with gastric carcinogenesis. However, the relationship between gastric fungi and GC is still unclear. Our aim was to evaluate the gastric fungal microbiota in the GC microenvironment. Methods. Gastric fungal microbiome profiling was performed with internal transcribed spacer (ITS) rDNA sequencing in primary tumor and corresponding paired normal mucosal tissues from 61 GC patients. Differences in microbial composition, taxa diversity, and predicted function were further analyzed. Results. Dysbiosis of gastric mucosal fungal microbiome was observed between the tumor and normal groups in GC. The tumor group had a higher abundance of certain taxa than the normal group. In the taxa classification, the abundances of Pezizomycetes, Sordariales, Chaetomiaceae, and Rozellomycota were lower in the tumor group than in the normal group. At the genus level, Solicoccozyma ( P = 0.033 ) was found in higher abundance and was differentially enriched in the tumor group with Lefse analysis. Additionally, Solicoccozyma accounted for 0.3% of gastric fungi in the GC microenvironment. Twenty-seven of the 61 GC patients showed positive Solicoccozyma expression in tumors. Solicoccozyma-positive expression in tumors was associated with the Bormann classification and nerve invasion. Solicoccozyma was considered a gastric fungal marker to classify stage I and stage II-IV GC patients with an area under the receiver-operating curve (AUC) of 0.7061, as well as to classify the nerve invasive and nonnerve invasive tumors from GC patients with an AUC of 0.6978. Functional prediction indicated that the positive expression of Solicoccozyma in tumors was associated with the amino acid- and carbohydrate-related metabolic pathways in GC. Conclusions. This study revealed a novel perspective on the role of Solicoccozyma in tumors and a theoretical basis for therapeutic targets against GC.

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“…Ecosystems yielding a higher level of biodiversity are also in general less vulnerable to disturbances than ecosystems that have a lower diversity [58]. Abundant interactions within a microbial community were shown to result in suppression of colonization by an invader E. coli O157:H7 [57,59]. Our study found a greater initial shift in the bacterial community composition in straw compared to straw + manure, in which inactivation of the added pathogens appeared to be more effective.…”

Section: Plos Onementioning

confidence: 56%

Medium-term storage of calf beddings affects bacterial community and effectiveness to inactivate zoonotic bacteria

Rapp,

Ross,

Cave

et al. 2023

PLoS ONE

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Land-spreading of animal faecal wastes -such as animal beddings- can introduce zoonotic enteropathogens into the food system environment. The study evaluated the effectiveness of animal beddings naturally contaminated by calf manure to reduce E. coli O157:H7 or Salmonella enterica. The two pathogens were introduced separately as a four strains-cocktail and at high (>6.5 Log10 g-1) concentration into bedding materials, and their inactivation over a 10 weeks-period was monitored by using a Most Probable Number (MPN) enumeration method. Inactivation of E. coli O157:H7 was more effective in the bedding inoculated immediately after collection from calf pens than in the beddings inoculated after a 2 months-pre-storage period: E. coli O157:H7 levels were reduced by 6.6 Log10 g-1 in unstored bedding (0.5 Log10 g-1 recovered; 95%CI: 0.0–1.2), and by 4.9 Log10 g-1 in pre-stored bedding (2.2 Log10 g-1 recovered; 95%CI: 1.5–2.8) with a significant (p<0.05) difference between unstored and pre-stored. S. enterica was inactivated less effectively as counts were reduced by one order of magnitude, with no significant difference in inactivation between unstored and pre-stored beddings. Low levels of naturally occurring E. coli O157 and Salmonella spp. were detected in the non-inoculated beddings, as well as in the straw prior to use in the animal facility. To better understand the possible biological processes involved, the bacterial community present in the beddings was characterised by short-read 16S rRNA sequencing. Pre-storage of the bedding affected the composition but not the diversity of the bacterial community. Analyses of the key bacterial phyla suggested that the presence of a diverse and stable bacterial community might facilitate inactivation of the introduced pathogens, and a possible role of bacterial orders associated with lignocellulolytic resources. Overall, the study contributed to the understanding of the fate of zoonotic bacteria introduced in animal beddings during storage and identified bedding storage practices pre-and post-use in animal facilities that could be important to prevent the risk of zoonosis dissemination to the environment or to the dairy herds.

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Disentangling survival of Escherichia coli O157:H7 in soils: From a subpopulation perspective

Cited by 13 publications

References 52 publications

The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland

The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland

Dysbiosis of Gastric Mucosal Fungal Microbiota in the Gastric Cancer Microenvironment

Medium-term storage of calf beddings affects bacterial community and effectiveness to inactivate zoonotic bacteria

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